Click here to switch to the map view.

The map label for this gene is nudF [H]

Identifier: 27364070

GI number: 27364070

Start: 623689

End: 624324

Strand: Reverse

Name: nudF [H]

Synonym: VV1_0610

Alternate gene names: 27364070

Gene position: 624324-623689 (Counterclockwise)

Preceding gene: 27364072

Following gene: 27364069

Centisome position: 19.02

GC content: 45.75

Gene sequence:

>636_bases
ATGCAACAGGATGACAAACAACACGCTGTTTTTACTCCAGAAGATGTAGAGGTTCTCTCAAAAGAGCCACTCTTTCAGGG
ATTCTTCAAGATGATCAAGTACCGCTTCCGTCATAAGTTATTTGCCGGTGGGTGGAGCAATGTGATCGAACGCGAAATGT
TTGAACGTGGTCATGCTGCTGCAATGCTTCCTTACGATCCTATTCGTGATCAAGTGGTGTTAATTGAACAAATTCGTGTC
GGAGCACTGGAGCATCAGCATCCTTGGCAGTTGGAAATTGTCGCAGGCATGATCGATCGTGATGAATCGGCGGATGATGT
CGTGCGTCGAGAGGCGCAAGAAGAAGCTGGCCTAGAAGTCGGACAACTTGTTTCGGTGGTTTCGTATTATCCTTCAGCGG
GTGGGTGTTCAGAAAAGCTGGATGTTTTTATCGGTGAAGTGGATGCCTCTAAAGCGCATGGCATTCATGGTTTAGACTAT
GAAGATGAAGATATCCGTGTGCATGTGATGACACGTGAGCAAGCCTACGATTTGGTCAAACAAGGCAAAATCGAGAATGG
TGCCTCAATTATCGCCTTACAATGGTTAGAGTTAAATCACTTGCAGTTAAAGTCACAGTGGTTGGAGATTGAGTGA

Upstream 100 bases:

>100_bases
CTAAAAATTTTTAGAACAATTATATAAAAAGTTGAGCAATGTCCTTTGAGTCATGTTTAAACAGTGAGTAAACTACAAGA
TTCACTCAGTGAGGTGCGAA

Downstream 100 bases:

>100_bases
TGGGTTTAGCAGCAGCAAAACAACCTTATTATGTTGATTTGGCAGGGTTAATGAGAACCTATGAAACCAATTATGCCAAA
TTAAATGCTTTGCTGCCGTT

Product: ADP-ribose pyrophosphatase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MQQDDKQHAVFTPEDVEVLSKEPLFQGFFKMIKYRFRHKLFAGGWSNVIEREMFERGHAAAMLPYDPIRDQVVLIEQIRV
GALEHQHPWQLEIVAGMIDRDESADDVVRREAQEEAGLEVGQLVSVVSYYPSAGGCSEKLDVFIGEVDASKAHGIHGLDY
EDEDIRVHVMTREQAYDLVKQGKIENGASIIALQWLELNHLQLKSQWLEIE

Sequences:

>Translated_211_residues
MQQDDKQHAVFTPEDVEVLSKEPLFQGFFKMIKYRFRHKLFAGGWSNVIEREMFERGHAAAMLPYDPIRDQVVLIEQIRV
GALEHQHPWQLEIVAGMIDRDESADDVVRREAQEEAGLEVGQLVSVVSYYPSAGGCSEKLDVFIGEVDASKAHGIHGLDY
EDEDIRVHVMTREQAYDLVKQGKIENGASIIALQWLELNHLQLKSQWLEIE
>Mature_211_residues
MQQDDKQHAVFTPEDVEVLSKEPLFQGFFKMIKYRFRHKLFAGGWSNVIEREMFERGHAAAMLPYDPIRDQVVLIEQIRV
GALEHQHPWQLEIVAGMIDRDESADDVVRREAQEEAGLEVGQLVSVVSYYPSAGGCSEKLDVFIGEVDASKAHGIHGLDY
EDEDIRVHVMTREQAYDLVKQGKIENGASIIALQWLELNHLQLKSQWLEIE

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=208, Percent_Identity=55.7692307692308, Blast_Score=240, Evalue=5e-65,
Organism=Escherichia coli, GI1788810, Length=155, Percent_Identity=37.4193548387097, Blast_Score=94, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 24158; Mature: 24158

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQDDKQHAVFTPEDVEVLSKEPLFQGFFKMIKYRFRHKLFAGGWSNVIEREMFERGHAA
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCE
AMLPYDPIRDQVVLIEQIRVGALEHQHPWQLEIVAGMIDRDESADDVVRREAQEEAGLEV
EECCCCCCCCCEEHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCH
GQLVSVVSYYPSAGGCSEKLDVFIGEVDASKAHGIHGLDYEDEDIRVHVMTREQAYDLVK
HHHHHHHHHCCCCCCCCHHHHEEEECCCCCHHCCCCCCCCCCCCEEEEEEEHHHHHHHHH
QGKIENGASIIALQWLELNHLQLKSQWLEIE
HCCCCCCCEEEEEEEECCCHHHHHHHHCCCC
>Mature Secondary Structure
MQQDDKQHAVFTPEDVEVLSKEPLFQGFFKMIKYRFRHKLFAGGWSNVIEREMFERGHAA
CCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCE
AMLPYDPIRDQVVLIEQIRVGALEHQHPWQLEIVAGMIDRDESADDVVRREAQEEAGLEV
EECCCCCCCCCEEHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCHHHHHHHHHHHHHCCCH
GQLVSVVSYYPSAGGCSEKLDVFIGEVDASKAHGIHGLDYEDEDIRVHVMTREQAYDLVK
HHHHHHHHHCCCCCCCCHHHHEEEECCCCCHHCCCCCCCCCCCCEEEEEEEHHHHHHHHH
QGKIENGASIIALQWLELNHLQLKSQWLEIE
HCCCCCCCEEEEEEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]