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The map label for this gene is murD

Identifier: 27364041

GI number: 27364041

Start: 594263

End: 595591

Strand: Reverse

Name: murD

Synonym: VV1_0580

Alternate gene names: 27364041

Gene position: 595591-594263 (Counterclockwise)

Preceding gene: 27364042

Following gene: 326423734

Centisome position: 18.15

GC content: 48.76

Gene sequence:

>1329_bases
ATGGATCGTTGGCAAGACATCAAACAGGTTGTCGTGGTAGGGCTCGGTATTACTGGGCTCTCCGTCGTTAGACATTTGCG
AAAAACACAGCCGCAGTTGCAAGTGAAAGTGATTGATACGCGTCCAACCCCTCCAGGTGTGGAACAATTGCCTCCAGACA
TTGCGCTGCATGTCGGCAGTTGGAATGACGCTTGGTTAGCTGAGGCCGATTTGGTCGTCACAAACCCAGGTATTGCCTTA
GCGACGCCTCAAATTCAAACGGTGTTAGCACGCGGCGTTGCTGTGGTGGGTGATATTGAGTTGTTCGCTTGGGCGGCAGA
TAAACCCGTCATCGCGATTACTGGCTCAAATGGCAAAAGCACCGTCACGGATTTAACCGGAGTCATGGCTAACGCCTGTG
GGGTGAAATGCGCAATTGGCGGTAACATTGGTGTTCCTGCACTCGATTTGTTGGAGCAAGAGGTTGAGCTTTATGTACTT
GAACTCTCGAGTTTTCAATTAGAGACGACTTCCAGCCTCCACTTGGTTGCAGCGGCATTTCTGAATCTTTCTGAAGATCA
CATGGATCGCTACCAAGGCATGGATGACTATCGCCAAGCTAAACTGCGCATCTTTCAGCACGCAAAACATGGAGTCGTGA
ATCGCGATGATCGTCAGACTTATCCTGAAACTTCCCATGGCCAGCAATCACTTGCTTTGGTGACTTTTGGCTCCGATGAC
AAAGAGTTTGGTGTCATAAGTCATCAGGGGGAATCTTGGTTGTCATATAACCAGCAACCTATTTTGGCAAGCCGAGAACT
CAAATTAGTCGGTCAGCACAACGTGGCAAACGTATTGGTTGTTTTGGCGCTGCTGACGTGCGCTGGTATTGATTACCGTA
AAGGCTTGAGCGCTTTGAAGTCTTACACCGGTTTGACACACCGATGTCAGGTTGTCGCAGATAATCGTGGCATCAAGTGG
GTCAATGATTCAAAAGCGACAAATCTTGCTAGTACACAGGCTGCCTTATCGGGGCTTAACTGTGCTGGCAAACTCTACCT
GCTGGTGGGTGGAGATGGCAAAGGGGCTGACTTTTCACCATTGAAGCCGATTTTGGCGCAGCTAAACCTCCAACTATGTT
GTTTTGGTGCTGATGGTGATCAATTTATGCCATTGCACGCATCGGCCACGCGTTTTGAGCGGATGGAAGATGTGATTGAA
CATATATCTCCACAATTACAATCTGGAGATATGGTGATGCTTTCCCCTGCATGTGCTAGTTTTGACCAATTCAGTAATTT
TATGGCGCGAGGCGATCGATTCGCCGAATTGGCTCGCCAGTATGCATAG

Upstream 100 bases:

>100_bases
TGTGCGTTTCTGGATTATTTCTATGGTGTTGGTTCTGATCGGTCTAGCGACACTGAAAGTGCGTTAATCGACGGACACTC
TTTTTACGGTGTAATGAAAT

Downstream 100 bases:

>100_bases
CGCAACCTCTAACGCGTATTTTTAACAAGGCACGATGTTTGGTGATGGTAGGTAATATCAAAAACAATTTGTTCGGCTGG
TTTCGCCACTCACCACCAGA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGSWNDAWLAEADLVVTNPGIAL
ATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKSTVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVL
ELSSFQLETTSSLHLVAAAFLNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
KEFGVISHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALKSYTGLTHRCQVVADNRGIKW
VNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSPLKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIE
HISPQLQSGDMVMLSPACASFDQFSNFMARGDRFAELARQYA

Sequences:

>Translated_442_residues
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGSWNDAWLAEADLVVTNPGIAL
ATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKSTVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVL
ELSSFQLETTSSLHLVAAAFLNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
KEFGVISHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALKSYTGLTHRCQVVADNRGIKW
VNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSPLKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIE
HISPQLQSGDMVMLSPACASFDQFSNFMARGDRFAELARQYA
>Mature_442_residues
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGSWNDAWLAEADLVVTNPGIAL
ATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKSTVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVL
ELSSFQLETTSSLHLVAAAFLNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
KEFGVISHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALKSYTGLTHRCQVVADNRGIKW
VNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSPLKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIE
HISPQLQSGDMVMLSPACASFDQFSNFMARGDRFAELARQYA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=437, Percent_Identity=52.6315789473684, Blast_Score=426, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_VIBVU (Q8DEK8)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759569.1
- ProteinModelPortal:   Q8DEK8
- SMR:   Q8DEK8
- GeneID:   1177531
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0580
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK04663
- BioCyc:   VVUL216895:VV1_0580-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 47932; Mature: 47932

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCEEEEECC
WNDAWLAEADLVVTNPGIALATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKS
CCCCEEEECCEEEECCCCEEECHHHHHHHHCCEEEEECEEEEEEECCCCEEEEECCCCCC
TVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVLELSSFQLETTSSLHLVAAAF
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHEEEEEEECCEEEECCCHHHHHHHHH
LNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
HCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
KEFGVISHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALK
CCCCEEECCCCHHHCCCCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
SYTGLTHRCQVVADNRGIKWVNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSP
HHCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCH
LKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIEHISPQLQSGDMVMLSPACAS
HHHHHHHCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHC
FDQFSNFMARGDRFAELARQYA
HHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MDRWQDIKQVVVVGLGITGLSVVRHLRKTQPQLQVKVIDTRPTPPGVEQLPPDIALHVGS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCEEEEECC
WNDAWLAEADLVVTNPGIALATPQIQTVLARGVAVVGDIELFAWAADKPVIAITGSNGKS
CCCCEEEECCEEEECCCCEEECHHHHHHHHCCEEEEECEEEEEEECCCCEEEEECCCCCC
TVTDLTGVMANACGVKCAIGGNIGVPALDLLEQEVELYVLELSSFQLETTSSLHLVAAAF
HHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHEEEEEEECCEEEECCCHHHHHHHHH
LNLSEDHMDRYQGMDDYRQAKLRIFQHAKHGVVNRDDRQTYPETSHGQQSLALVTFGSDD
HCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
KEFGVISHQGESWLSYNQQPILASRELKLVGQHNVANVLVVLALLTCAGIDYRKGLSALK
CCCCEEECCCCHHHCCCCCCCEECCCEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
SYTGLTHRCQVVADNRGIKWVNDSKATNLASTQAALSGLNCAGKLYLLVGGDGKGADFSP
HHCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCH
LKPILAQLNLQLCCFGADGDQFMPLHASATRFERMEDVIEHISPQLQSGDMVMLSPACAS
HHHHHHHCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHC
FDQFSNFMARGDRFAELARQYA
HHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA