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The map label for this gene is carA [H]

Identifier: 27364028

GI number: 27364028

Start: 579428

End: 580567

Strand: Reverse

Name: carA [H]

Synonym: VV1_0566

Alternate gene names: 27364028

Gene position: 580567-579428 (Counterclockwise)

Preceding gene: 27364029

Following gene: 27364027

Centisome position: 17.69

GC content: 47.28

Gene sequence:

>1140_bases
TTGAGTAAATCAGCACTGTTAGTCCTGGAAGATGGGACAGTGTTCCGCGGAGTGTCCATTGGCGCAGATGGTATTTCCGT
TGGAGAAGTCGTTTTCAATACCTCGATGACGGGGTATCAAGAAATCCTCACTGATCCTTCTTATTCCCAGCAAATCGTTA
CTCTTACTTATCCTCACATTGGCAATACCGGAACCACTTCCGAAGACGAAGAATCCTCTTCCATTCATGCTCAAGGCCTT
GTGATTCGCGATCTTCCTCTTATCGCTTCTAACTTCCGTAGTGAACAATCTCTTTCTGATTACCTCAAATCGCAAAACAT
TGTCGGCATTGCTGATATTGATACGCGTAAGCTCACTCGTATTCTGCGTGAGAAAGGTGCACAAAACGGTTGTATCGTTG
CAGGTAACAACCTAGATGAAGCTTTGGCACTGGCGAAAGCAAAAGAATTCCCAGGTTTAAAAGGAATGGATCTTGCGAAA
GAGGTTACAACAAAAGAAGCGTATCAATGGAAACAAGGTTCGTGGACGCTTGAGGGTGGACTTCCAGAAGCGAAAGATGA
CAGCGAATTGCCATATCACGTTGTTGCCTACGATTTCGGCGCAAAACGCAACATCTTACGAATGTTGGTTGACCGCGGCT
GCCGCTTAACGGTCGTGCCAGCAGAAACGTCGGCAGAAGACGTTCTGGCGCTAAACCCAGATGGTGTTTTCCTGTCAAAC
GGCCCTGGTGACCCAGCACCTTGTACTTATGCGATTGAAGCAACCAAAGTGTTCCTAGAAAATGGCCTACCAGTATTTGG
TATTTGTCTTGGCCACCAAATTCTCGCGTTGGCGTCTGGTGCGAAAACGGTGAAGATGAAGTTTGGTCACCATGGTGCAA
ACCATCCTGTAAAAGATTTAGACCGCAATGTGGTGATGATTACTTCGCAAAACCACGGTTTTGCCGCTGATGAAGCAACT
CTACCAGATAACCTACGTGCTACTCACGTATCGCTCTTTGATGGCTCTTTGCAGGGAATTCACCGCACAGACAAGCCAGC
ATTCAGCTTCCAAGGTCACCCTGAAGCGAGCCCAGGTCCACACGACGCAGCTCCGCTATTTGACCATTTCATCGAACTGA
TTAAACAACACAGCGCTTAA

Upstream 100 bases:

>100_bases
CAATTTGTCTGAATTCCCCAAAATCACACTTTTCTGGTGATAGATAGGGCAGATTTGCAGTTTAATTTATTTTTTATGCA
TTTTTATTCTGGAGGTTGTC

Downstream 100 bases:

>100_bases
TTTGGAGTAGTAGACAATGCCAAAACGTACTGACATTCAAAGCATTCTTATCCTAGGTGCTGGTCCAATTGTTATCGGTC
AGGCTTGTGAGTTTGACTAC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTTSEDEESSSIHAQGL
VIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAK
EVTTKEAYQWKQGSWTLEGGLPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSN
GPGDPAPCTYAIEATKVFLENGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRNVVMITSQNHGFAADEAT
LPDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQHSA

Sequences:

>Translated_379_residues
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTTSEDEESSSIHAQGL
VIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAK
EVTTKEAYQWKQGSWTLEGGLPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSN
GPGDPAPCTYAIEATKVFLENGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRNVVMITSQNHGFAADEAT
LPDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQHSA
>Mature_378_residues
SKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHIGNTGTTSEDEESSSIHAQGLV
IRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTRILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAKE
VTTKEAYQWKQGSWTLEGGLPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSNG
PGDPAPCTYAIEATKVFLENGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDLDRNVVMITSQNHGFAADEATL
PDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIKQHSA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=386, Percent_Identity=40.1554404145078, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI169790915, Length=391, Percent_Identity=36.8286445012788, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI21361331, Length=391, Percent_Identity=36.8286445012788, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=82.4933687002653, Blast_Score=659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=385, Percent_Identity=36.8831168831169, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324878, Length=391, Percent_Identity=39.6419437340153, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=391, Percent_Identity=36.8286445012788, Blast_Score=223, Evalue=3e-59,
Organism=Drosophila melanogaster, GI45555749, Length=394, Percent_Identity=40.1015228426396, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24642586, Length=394, Percent_Identity=40.1015228426396, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40816; Mature: 40685

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCCEEEEEECCCEEEEEEECCCCCCHHHEEEECCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTTSEDEESSSIHAQGLVIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTR
CCCCCCCCCCCCCCEEECCEEEECCHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHH
ILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAKEVTTKEAYQWKQGSWTLEGG
HHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEEECC
LPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSN
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEC
GPGDPAPCTYAIEATKVFLENGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDL
CCCCCCCEEEEEHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHC
DRNVVMITSQNHGFAADEATLPDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGP
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HDAAPLFDHFIELIKQHSA
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKSALLVLEDGTVFRGVSIGADGISVGEVVFNTSMTGYQEILTDPSYSQQIVTLTYPHI
CCCEEEEEECCCEEEEEEECCCCCCHHHEEEECCCCHHHHHHCCCCCCCEEEEEECCCC
GNTGTTSEDEESSSIHAQGLVIRDLPLIASNFRSEQSLSDYLKSQNIVGIADIDTRKLTR
CCCCCCCCCCCCCCEEECCEEEECCHHHHHHCCCHHHHHHHHHCCCEEEEECCCHHHHHH
ILREKGAQNGCIVAGNNLDEALALAKAKEFPGLKGMDLAKEVTTKEAYQWKQGSWTLEGG
HHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCCEEEECC
LPEAKDDSELPYHVVAYDFGAKRNILRMLVDRGCRLTVVPAETSAEDVLALNPDGVFLSN
CCCCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCCEEEEC
GPGDPAPCTYAIEATKVFLENGLPVFGICLGHQILALASGAKTVKMKFGHHGANHPVKDL
CCCCCCCEEEEEHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHC
DRNVVMITSQNHGFAADEATLPDNLRATHVSLFDGSLQGIHRTDKPAFSFQGHPEASPGP
CCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
HDAAPLFDHFIELIKQHSA
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA