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The map label for this gene is trmJ [H]

Identifier: 27363908

GI number: 27363908

Start: 435499

End: 436227

Strand: Reverse

Name: trmJ [H]

Synonym: VV1_0440

Alternate gene names: 27363908

Gene position: 436227-435499 (Counterclockwise)

Preceding gene: 27363910

Following gene: 326423712

Centisome position: 13.29

GC content: 48.56

Gene sequence:

>729_bases
ATGTTAGATAAAGTAAAAGTCGTCCTTGTTGGCACCTCTCACTCCGGAAATATTGGGTCTGCAGCTCGTGCCATGAAGGT
GATGGGTTTATCGCAGCTTGTGCTTGTTCAGCCTGAATGTGAGGTTGACGCTCAAGCTATCGCTTTGGCATCGGGAGCGG
CGGATATCGCATTGAATGCGTTAGTGGTGAATTCACTGGAAGAAGCGGTGGCCGATTGTGCGCTGGTTGTCGGTTCAAGT
GCCCGTTCTCGTACGCTTGAGTGGCCGATGCTTGAACCGTTTGAATGCGCGGAAAAGTTTGTCCAAGAGGGGCAAACGAG
TCATGTTGCCTTGGTATTTGGCCGTGAACGTACCGGTCTCACCAACGATGAGCTTCAACGTTGCCATTATCACGTTTGTA
TTCCAGCAAACCCAGAATACAGCTCCTTGAATCTCGCGATGGCAGTACAGACCCTAAGTTACGAAGTGCGTATGGCTTAT
TTGCGTCATCAACAAAGCCTATATCCAACGCAAGAAACCGCGGAATACCCACGCCACAGCGAACTTGAACTGTTTTATCA
GCACTTAGAGAAAGTGCTACTGAAAACCGAATTTGTGCGAGCAGATCAGCCTAACCAAGTCATGAATAAAATGCGTCGAC
TGTTCAGCCGAGCTCGTCCAGAGGCGCAAGAGTTGAACATTTTGCGTGGCATTTTGACTTCGGTAGAAAAGAGCATTAAT
GAGCAATAG

Upstream 100 bases:

>100_bases
AAGAGAGGGGGGGAGGCTTGTTCTCGTCATATCCAATATAGGGTGTGGGAAAAAACTATGATAAAATCCCGCGGTTTGAA
ATCAGCCATTGAAAGTTTGC

Downstream 100 bases:

>100_bases
TATCCCTGCTATTCATAGGGATGAATACCTGACTATTTTAGTCAAATAAATACTTGACCATTTTGGTCAGGTATGGAAAA
ATCCGTATCATTACAAACAG

Product: tRNA:Cm32/Um32 methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MLDKVKVVLVGTSHSGNIGSAARAMKVMGLSQLVLVQPECEVDAQAIALASGAADIALNALVVNSLEEAVADCALVVGSS
ARSRTLEWPMLEPFECAEKFVQEGQTSHVALVFGRERTGLTNDELQRCHYHVCIPANPEYSSLNLAMAVQTLSYEVRMAY
LRHQQSLYPTQETAEYPRHSELELFYQHLEKVLLKTEFVRADQPNQVMNKMRRLFSRARPEAQELNILRGILTSVEKSIN
EQ

Sequences:

>Translated_242_residues
MLDKVKVVLVGTSHSGNIGSAARAMKVMGLSQLVLVQPECEVDAQAIALASGAADIALNALVVNSLEEAVADCALVVGSS
ARSRTLEWPMLEPFECAEKFVQEGQTSHVALVFGRERTGLTNDELQRCHYHVCIPANPEYSSLNLAMAVQTLSYEVRMAY
LRHQQSLYPTQETAEYPRHSELELFYQHLEKVLLKTEFVRADQPNQVMNKMRRLFSRARPEAQELNILRGILTSVEKSIN
EQ
>Mature_242_residues
MLDKVKVVLVGTSHSGNIGSAARAMKVMGLSQLVLVQPECEVDAQAIALASGAADIALNALVVNSLEEAVADCALVVGSS
ARSRTLEWPMLEPFECAEKFVQEGQTSHVALVFGRERTGLTNDELQRCHYHVCIPANPEYSSLNLAMAVQTLSYEVRMAY
LRHQQSLYPTQETAEYPRHSELELFYQHLEKVLLKTEFVRADQPNQVMNKMRRLFSRARPEAQELNILRGILTSVEKSIN
EQ

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=238, Percent_Identity=63.0252100840336, Blast_Score=310, Evalue=6e-86,
Organism=Escherichia coli, GI1790865, Length=154, Percent_Identity=36.3636363636364, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27000; Mature: 27000

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKVKVVLVGTSHSGNIGSAARAMKVMGLSQLVLVQPECEVDAQAIALASGAADIALNA
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHH
LVVNSLEEAVADCALVVGSSARSRTLEWPMLEPFECAEKFVQEGQTSHVALVFGRERTGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCC
TNDELQRCHYHVCIPANPEYSSLNLAMAVQTLSYEVRMAYLRHQQSLYPTQETAEYPRHS
CHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
ELELFYQHLEKVLLKTEFVRADQPNQVMNKMRRLFSRARPEAQELNILRGILTSVEKSIN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
EQ
CC
>Mature Secondary Structure
MLDKVKVVLVGTSHSGNIGSAARAMKVMGLSQLVLVQPECEVDAQAIALASGAADIALNA
CCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHH
LVVNSLEEAVADCALVVGSSARSRTLEWPMLEPFECAEKFVQEGQTSHVALVFGRERTGL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCC
TNDELQRCHYHVCIPANPEYSSLNLAMAVQTLSYEVRMAYLRHQQSLYPTQETAEYPRHS
CHHHHHHCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
ELELFYQHLEKVLLKTEFVRADQPNQVMNKMRRLFSRARPEAQELNILRGILTSVEKSIN
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
EQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA