Click here to switch to the map view.

The map label for this gene is guaA [H]

Identifier: 27363886

GI number: 27363886

Start: 411874

End: 413427

Strand: Reverse

Name: guaA [H]

Synonym: VV1_0418

Alternate gene names: 27363886

Gene position: 413427-411874 (Counterclockwise)

Preceding gene: 326423708

Following gene: 27363885

Centisome position: 12.6

GC content: 46.78

Gene sequence:

>1554_bases
ATGACTAAAAATATTCATGACCAACGTATTCTGATCCTAGATTTCGGTTCGCAGTACACTCAACTTGTTGCGCGCCGCGT
ACGTGAAATCGGCGTTTACTGTGAGCTTTGGAGCTGGGATGTTGAAGAAGCGGATATTCGTGAATTCAACCCAGACGGTA
TTATTCTTTCTGGTGGCCCAGAGAGCGTGACAGAAGATAACTCTCCTCGCGCACCTCAATATGTATTCGATTCAGGCGTG
CCAGTACTGGGTGTTTGTTACGGCATGCAGACCATGGCTGAGCAGCTAGGCGGAAAAGTATCGACTTCTGACGAGCGCGA
GTTTGGCTATGCAGCTGTAAAAGTGTCTGGTGAGTCAGCTATTTTCAAAGATCTTGAAGCTACTCAAGATGTATGGATGA
GCCACGGTGACAAAGTCGTTGAGATTCCAGCGGGCTTCACCAAAGTAGGTGAAACAGACACTTGTCCTTACGCTGCAATG
GCGAACGAAGAGAAGAAATACTACGGTGTTCAGTTCCACCCAGAAGTAACGCACACTAAAAATGGCTTACAAATGCTAGA
GAACTTTGTTCTTGGCATCTGTGGTTGTGAGCGTCTATGGACTTCAGAATCCATCATCGAAGATGCGGTTGCTCGTATTA
AAGAGCAAGTGGGTGACGATGAAGTGATCCTAGGTCTATCTGGTGGTGTGGATTCATCAGTAGTAGCCATGTTGGTTCAC
CGTGCAATCGGCGACAAGCTAACGTGTGTCTTCGTTGATAACGGTCTACTTCGTTTAAACGAAGGTCAGCAAGTGATGGA
CATGTTTGGCGACAAGTTTGGCCTAAACATCATCAAAGTTGATGCTGAAGAGCGTTTCCTAAAAGCACTTGAAGGCAAAT
CTGATCCTGAAGAGAAACGTAAGACCATCGGTCACGTATTCGTCGATGTCTTTGATGAAGAATCGAAGAAGCTGAAAAAT
GCGAAATGGCTCGCTCAAGGTACTATCTACCCAGACGTAATCGAGTCAGCAGCCTCTAAGACAGGTAAAGCGCACGTAAT
CAAATCTCACCATAACGTGGGCGGTCTGCCAGACGATATGGAAATGGGTCTTGTTGAGCCACTGCGTGAACTGTTCAAAG
ATGAAGTACGTAAGATTGGCCTAGAACTTGGCCTACCTTACGAAATGCTTTACCGCCACCCATTCCCTGGTCCGGGTCTA
GGTGTTCGTGTTCTTGGTGAAATCAAGAAAGAGTACTGTGATTTGCTACGTCGTGCGGACGCTATCTTTATTGAAGAGCT
CCATGCTGCGGATCTTTACAACAAAGTATCTCAAGCGTTTACGGTATTCCTACCAGTGCGTTCTGTAGGTGTAATGGGCG
ATGGCCGTAAGTATGACTGGGTGGTATCACTACGTGCCGTAGAGACCATCGACTTCATGACGGCGCATTGGGCACACCTA
CCGTACGATTTCCTAGGTAAGGTTTCTAACCGTATTATCAATGAAGTAAACGGCATCTCACGCGTGGTTTACGATATCTC
TGGTAAGCCACCAGCAACCATCGAATGGGAATAA

Upstream 100 bases:

>100_bases
AACTATCGCTTGGGTTAATTGACCCTGAGTTGAAATTTAAAGCTTATGGGGAGCGAGATGGGCTCCCCATCCACCAAATT
TGATATCGAGATTAACCTCA

Downstream 100 bases:

>100_bases
TCTTGCGTTAACGCATTGCATTGATCTATCGAATAAACCCCGAGTGAACTCTCGGGGTTTTTTATTACTGTTTACTCGGG
TTGTTGGTTTTGGAACAGGG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGPESVTEDNSPRAPQYVFDSGV
PVLGVCYGMQTMAEQLGGKVSTSDEREFGYAAVKVSGESAIFKDLEATQDVWMSHGDKVVEIPAGFTKVGETDTCPYAAM
ANEEKKYYGVQFHPEVTHTKNGLQMLENFVLGICGCERLWTSESIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLVH
RAIGDKLTCVFVDNGLLRLNEGQQVMDMFGDKFGLNIIKVDAEERFLKALEGKSDPEEKRKTIGHVFVDVFDEESKKLKN
AKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYEMLYRHPFPGPGL
GVRVLGEIKKEYCDLLRRADAIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
PYDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE

Sequences:

>Translated_517_residues
MTKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGPESVTEDNSPRAPQYVFDSGV
PVLGVCYGMQTMAEQLGGKVSTSDEREFGYAAVKVSGESAIFKDLEATQDVWMSHGDKVVEIPAGFTKVGETDTCPYAAM
ANEEKKYYGVQFHPEVTHTKNGLQMLENFVLGICGCERLWTSESIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLVH
RAIGDKLTCVFVDNGLLRLNEGQQVMDMFGDKFGLNIIKVDAEERFLKALEGKSDPEEKRKTIGHVFVDVFDEESKKLKN
AKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYEMLYRHPFPGPGL
GVRVLGEIKKEYCDLLRRADAIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
PYDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
>Mature_516_residues
TKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGPESVTEDNSPRAPQYVFDSGVP
VLGVCYGMQTMAEQLGGKVSTSDEREFGYAAVKVSGESAIFKDLEATQDVWMSHGDKVVEIPAGFTKVGETDTCPYAAMA
NEEKKYYGVQFHPEVTHTKNGLQMLENFVLGICGCERLWTSESIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLVHR
AIGDKLTCVFVDNGLLRLNEGQQVMDMFGDKFGLNIIKVDAEERFLKALEGKSDPEEKRKTIGHVFVDVFDEESKKLKNA
KWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDMEMGLVEPLRELFKDEVRKIGLELGLPYEMLYRHPFPGPGLG
VRVLGEIKKEYCDLLRRADAIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLP
YDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP [H]

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain [H]

Homologues:

Organism=Homo sapiens, GI4504035, Length=554, Percent_Identity=36.2815884476534, Blast_Score=308, Evalue=1e-83,
Organism=Escherichia coli, GI1788854, Length=525, Percent_Identity=80, Blast_Score=881, Evalue=0.0,
Organism=Escherichia coli, GI1789760, Length=150, Percent_Identity=30.6666666666667, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71992717, Length=567, Percent_Identity=34.9206349206349, Blast_Score=297, Evalue=7e-81,
Organism=Caenorhabditis elegans, GI71992710, Length=567, Percent_Identity=34.9206349206349, Blast_Score=297, Evalue=8e-81,
Organism=Caenorhabditis elegans, GI133901714, Length=567, Percent_Identity=34.9206349206349, Blast_Score=297, Evalue=9e-81,
Organism=Saccharomyces cerevisiae, GI6323873, Length=515, Percent_Identity=49.7087378640777, Blast_Score=534, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6322638, Length=229, Percent_Identity=24.8908296943231, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281365319, Length=555, Percent_Identity=37.6576576576577, Blast_Score=305, Evalue=5e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR022310
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00117 GATase; PF00958 GMP_synt_C; PF02540 NAD_synthase [H]

EC number: =6.3.5.2 [H]

Molecular weight: Translated: 57676; Mature: 57545

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGP
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHCCCCCEEEECCCC
ESVTEDNSPRAPQYVFDSGVPVLGVCYGMQTMAEQLGGKVSTSDEREFGYAAVKVSGESA
CCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHH
IFKDLEATQDVWMSHGDKVVEIPAGFTKVGETDTCPYAAMANEEKKYYGVQFHPEVTHTK
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCEECEEECCCCCCCH
NGLQMLENFVLGICGCERLWTSESIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLVH
HHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
RAIGDKLTCVFVDNGLLRLNEGQQVMDMFGDKFGLNIIKVDAEERFLKALEGKSDPEEKR
HHHCCCEEEEEECCCEEEECCCHHHHHHCCHHHCCEEEEECHHHHHHHHHCCCCCHHHHH
KTIGHVFVDVFDEESKKLKNAKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDM
HHHHHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
EMGLVEPLRELFKDEVRKIGLELGLPYEMLYRHPFPGPGLGVRVLGEIKKEYCDLLRRAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
HHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHC
PYDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
CHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECC
>Mature Secondary Structure 
TKNIHDQRILILDFGSQYTQLVARRVREIGVYCELWSWDVEEADIREFNPDGIILSGGP
CCCCCCCEEEEEECCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHCCCCCEEEECCCC
ESVTEDNSPRAPQYVFDSGVPVLGVCYGMQTMAEQLGGKVSTSDEREFGYAAVKVSGESA
CCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHH
IFKDLEATQDVWMSHGDKVVEIPAGFTKVGETDTCPYAAMANEEKKYYGVQFHPEVTHTK
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCEECEEECCCCCCCH
NGLQMLENFVLGICGCERLWTSESIIEDAVARIKEQVGDDEVILGLSGGVDSSVVAMLVH
HHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
RAIGDKLTCVFVDNGLLRLNEGQQVMDMFGDKFGLNIIKVDAEERFLKALEGKSDPEEKR
HHHCCCEEEEEECCCEEEECCCHHHHHHCCHHHCCEEEEECHHHHHHHHHCCCCCHHHHH
KTIGHVFVDVFDEESKKLKNAKWLAQGTIYPDVIESAASKTGKAHVIKSHHNVGGLPDDM
HHHHHHHHHHHHHHHHHHHCCHHEECCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCHH
EMGLVEPLRELFKDEVRKIGLELGLPYEMLYRHPFPGPGLGVRVLGEIKKEYCDLLRRAD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
AIFIEELHAADLYNKVSQAFTVFLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHL
HHHHHHHHHHHHHHHHHHHHEEEEEHHHCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHC
PYDFLGKVSNRIINEVNGISRVVYDISGKPPATIEWE
CHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA