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The map label for this gene is grpE [H]

Identifier: 27363834

GI number: 27363834

Start: 349555

End: 350106

Strand: Reverse

Name: grpE [H]

Synonym: VV1_0365

Alternate gene names: 27363834

Gene position: 350106-349555 (Counterclockwise)

Preceding gene: 27363838

Following gene: 27363833

Centisome position: 10.67

GC content: 46.56

Gene sequence:

>552_bases
ATGAGCAACGAAGAAAACAAAATCAACGAAGAAGCGCTTAAACAGCAAGACGCTGCGGAAGTAGAAGTAGAAGCTGTTGG
CACTGATGCTGATATCGAATGGAACGAAGAGGCCGATGAATCAGCGGCGAAGATCGCTGAACTGGAAGCCGCTTTATTAG
CCAGCGAAGCGCGTGTGAAAGAGCAGCAAGATTCAGTGCTACGTGCGAAAGCAGAAGTGGAAAACATGCGTCGCCGTACT
GAGCAAGAAATTGATAAAGCGCGTAAATATGCGTTGAATCGCTTTGCTGAAGAACTGCTGCCAGTGATTGATAATCTAGA
GCGTGCGATTCAAGCGGCTGACGCTGAAAGCGAAGCGGTAAAACCGCTGCTTGAAGGCGTTGAATTGACTCATAAAACGT
TCGTTGATGTGGTCAGTAAGTTTGGTTTGAAAGAAATCAATCCAGAAGGCCAACCATTTAACCCAGAATGGCATCAAGCG
ATGTCGATTCAGGAAAGCCCAGACCACGAGTCAAATACCGTGATGTTCGTGATGCAAAAGGCTATGAATTGA

Upstream 100 bases:

>100_bases
ATAATAATGGCAAGTTATCTAACTTTGCGAATTTTTGTGAAGATAAAGCCCATAAAACGTGAGGGGCTTTAAGGACAAAT
AGTTGAATTCTGGAGATATC

Downstream 100 bases:

>100_bases
ATGGTCGAGTGATTCGTCCTGCGATGGTTATGGTTGCGAAATAAGCGAACTGATAGACCGATTCAAACACACAGAGCGGC
TTTTGCCGCTCTTTTTTATG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAAKIAELEAALLASEARVKEQQDSVLRAKAEVENMRRRT
EQEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAVKPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQA
MSIQESPDHESNTVMFVMQKAMN

Sequences:

>Translated_183_residues
MSNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAAKIAELEAALLASEARVKEQQDSVLRAKAEVENMRRRT
EQEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAVKPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQA
MSIQESPDHESNTVMFVMQKAMN
>Mature_182_residues
SNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAAKIAELEAALLASEARVKEQQDSVLRAKAEVENMRRRTE
QEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAVKPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQAM
SIQESPDHESNTVMFVMQKAMN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=127, Percent_Identity=33.0708661417323, Blast_Score=68, Evalue=4e-12,
Organism=Escherichia coli, GI1788967, Length=160, Percent_Identity=45, Blast_Score=129, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17552458, Length=104, Percent_Identity=33.6538461538462, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=151, Percent_Identity=34.4370860927152, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653432, Length=144, Percent_Identity=31.25, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20607; Mature: 20476

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAAKIAELEAALLASEARVK
CCCCCHHHHHHHHHHHHHHHEEHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQQDSVLRAKAEVENMRRRTEQEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQAMSIQESPDHESNTVMFVMQK
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHH
AMN
HCC
>Mature Secondary Structure 
SNEENKINEEALKQQDAAEVEVEAVGTDADIEWNEEADESAAKIAELEAALLASEARVK
CCCCHHHHHHHHHHHHHHHEEHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
EQQDSVLRAKAEVENMRRRTEQEIDKARKYALNRFAEELLPVIDNLERAIQAADAESEAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
KPLLEGVELTHKTFVDVVSKFGLKEINPEGQPFNPEWHQAMSIQESPDHESNTVMFVMQK
HHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHH
AMN
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA