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The map label for this gene is yafC [C]

Identifier: 27363815

GI number: 27363815

Start: 331668

End: 332552

Strand: Reverse

Name: yafC [C]

Synonym: VV1_0343

Alternate gene names: 27363815

Gene position: 332552-331668 (Counterclockwise)

Preceding gene: 326423693

Following gene: 27363814

Centisome position: 10.13

GC content: 49.72

Gene sequence:

>885_bases
ATGGCCAATATTCATTGGGAAGGTGTGAACGAGTTTGTTGCGGTGGCCGAAAGCGGCAGCTTCACTCAGGCCGCCACGCG
CTTAGAAACATCGGTCGCAAATGTGAGCCGCCGAGTCGGACAACTGGAAGAGCGGTTAGGTGTGAAGCTGTTACTGCGCT
CAACAAGAAAAGTGTCACTGACTGAGGCTGGGCAGATCTACGTTCAGCATTGTCGCAGCATATTGGAAGGGCTTCATCAA
GCGGAGCTAGCCGTGACACAAATGCAGCAGACGCCACAAGGGAAATTAAGAATTACCGCCCCGGTCACTTACGGTGAGCA
AAAGATCGCGCCGTTGCTGAATGACTTTCTGATTCGTTACCCTAAAATTGATTTAGAGTTAGTGCTCACCAACCAAAAGC
TCGACTTGATTGAGCAGGGTGTGGATATCGCGGTTCGGCTGGGTCAGCTTGAAGATTCTAGTTCCATGGCGCGTCGACTA
AGTGATCGTCATCTGCATGTTTGTGCGACGCCAGAATATTTTGAACAGCATGGCATGCCTCACACGTTATCGGATTTGTC
GTCACACAACTGCCTGATCGGTACCCACGATCATTGGCGTTTCAAAGAGAATCATCAAGGTCGTTCCATTAAAGTGAATG
GGCGTATTCGTTGCTCTAGTGGTGTTGTGCTATTGGATGCCGCTCTGAAAGGGATTGGTTTGGTTCAGTTACCGGATTAC
TACGTTGATGAGCACTTGCACGCAGGGCGACTTATCGAAGTACTGGCGCATTTTCGTGATGATCGTGAAGGGGTTTGGGC
GCTGTATCCACACAATCGACATTTATCGCCGAAAGTTCGTTTGCTGGTGGATCATCTGGCACAACATTTATCTGCAGCCG
CATAA

Upstream 100 bases:

>100_bases
CCAAGAATTTCAGTATAGTCATTCCAAATTGTTTTATAATCCTGTTAAATTGAGAATGACTTTTACACATGAGTAATAAT
GAATGTGAAGGAGAAGTGTG

Downstream 100 bases:

>100_bases
CCGCTATAATCCCCAACCCAGACGATAGAGGTAAACATGGCAGAGACACATCAACCCTTTTCACTTCAAGATGAAGTTTT
TATGCGCAGAGCGATTGAGC

Product: transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MANIHWEGVNEFVAVAESGSFTQAATRLETSVANVSRRVGQLEERLGVKLLLRSTRKVSLTEAGQIYVQHCRSILEGLHQ
AELAVTQMQQTPQGKLRITAPVTYGEQKIAPLLNDFLIRYPKIDLELVLTNQKLDLIEQGVDIAVRLGQLEDSSSMARRL
SDRHLHVCATPEYFEQHGMPHTLSDLSSHNCLIGTHDHWRFKENHQGRSIKVNGRIRCSSGVVLLDAALKGIGLVQLPDY
YVDEHLHAGRLIEVLAHFRDDREGVWALYPHNRHLSPKVRLLVDHLAQHLSAAA

Sequences:

>Translated_294_residues
MANIHWEGVNEFVAVAESGSFTQAATRLETSVANVSRRVGQLEERLGVKLLLRSTRKVSLTEAGQIYVQHCRSILEGLHQ
AELAVTQMQQTPQGKLRITAPVTYGEQKIAPLLNDFLIRYPKIDLELVLTNQKLDLIEQGVDIAVRLGQLEDSSSMARRL
SDRHLHVCATPEYFEQHGMPHTLSDLSSHNCLIGTHDHWRFKENHQGRSIKVNGRIRCSSGVVLLDAALKGIGLVQLPDY
YVDEHLHAGRLIEVLAHFRDDREGVWALYPHNRHLSPKVRLLVDHLAQHLSAAA
>Mature_293_residues
ANIHWEGVNEFVAVAESGSFTQAATRLETSVANVSRRVGQLEERLGVKLLLRSTRKVSLTEAGQIYVQHCRSILEGLHQA
ELAVTQMQQTPQGKLRITAPVTYGEQKIAPLLNDFLIRYPKIDLELVLTNQKLDLIEQGVDIAVRLGQLEDSSSMARRLS
DRHLHVCATPEYFEQHGMPHTLSDLSSHNCLIGTHDHWRFKENHQGRSIKVNGRIRCSSGVVLLDAALKGIGLVQLPDYY
VDEHLHAGRLIEVLAHFRDDREGVWALYPHNRHLSPKVRLLVDHLAQHLSAAA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=287, Percent_Identity=36.9337979094077, Blast_Score=178, Evalue=3e-46,
Organism=Escherichia coli, GI1789440, Length=281, Percent_Identity=31.6725978647687, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1789639, Length=284, Percent_Identity=34.8591549295775, Blast_Score=160, Evalue=7e-41,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=33.7883959044369, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081978, Length=285, Percent_Identity=33.3333333333333, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=31.9148936170213, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787128, Length=281, Percent_Identity=31.6725978647687, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI157672245, Length=125, Percent_Identity=40.8, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788706, Length=251, Percent_Identity=28.6852589641434, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786448, Length=266, Percent_Identity=24.4360902255639, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789173, Length=247, Percent_Identity=27.9352226720648, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790011, Length=255, Percent_Identity=24.7058823529412, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786713, Length=119, Percent_Identity=38.655462184874, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788887, Length=255, Percent_Identity=27.0588235294118, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788297, Length=140, Percent_Identity=36.4285714285714, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=289, Percent_Identity=25.6055363321799, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787806, Length=116, Percent_Identity=34.4827586206897, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=150, Percent_Identity=32.6666666666667, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87082132, Length=295, Percent_Identity=23.728813559322, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33047; Mature: 32916

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIHWEGVNEFVAVAESGSFTQAATRLETSVANVSRRVGQLEERLGVKLLLRSTRKVSL
CCCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEH
TEAGQIYVQHCRSILEGLHQAELAVTQMQQTPQGKLRITAPVTYGEQKIAPLLNDFLIRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHC
PKIDLELVLTNQKLDLIEQGVDIAVRLGQLEDSSSMARRLSDRHLHVCATPEYFEQHGMP
CCCCEEEEEECCHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEEEECHHHHHHCCCC
HTLSDLSSHNCLIGTHDHWRFKENHQGRSIKVNGRIRCSSGVVLLDAALKGIGLVQLPDY
CCHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCEEEECCCEEEEEHHHHCCCEEECCCH
YVDEHLHAGRLIEVLAHFRDDREGVWALYPHNRHLSPKVRLLVDHLAQHLSAAA
HHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANIHWEGVNEFVAVAESGSFTQAATRLETSVANVSRRVGQLEERLGVKLLLRSTRKVSL
CCCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEH
TEAGQIYVQHCRSILEGLHQAELAVTQMQQTPQGKLRITAPVTYGEQKIAPLLNDFLIRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHC
PKIDLELVLTNQKLDLIEQGVDIAVRLGQLEDSSSMARRLSDRHLHVCATPEYFEQHGMP
CCCCEEEEEECCHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCEEEEECHHHHHHCCCC
HTLSDLSSHNCLIGTHDHWRFKENHQGRSIKVNGRIRCSSGVVLLDAALKGIGLVQLPDY
CCHHHHCCCCEEEECCCCCCCCCCCCCCEEEECCEEEECCCEEEEEHHHHCCCEEECCCH
YVDEHLHAGRLIEVLAHFRDDREGVWALYPHNRHLSPKVRLLVDHLAQHLSAAA
HHHHHCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]