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The map label for this gene is tadA [H]

Identifier: 27363814

GI number: 27363814

Start: 331086

End: 331631

Strand: Reverse

Name: tadA [H]

Synonym: VV1_0342

Alternate gene names: 27363814

Gene position: 331631-331086 (Counterclockwise)

Preceding gene: 27363815

Following gene: 27363812

Centisome position: 10.1

GC content: 49.82

Gene sequence:

>546_bases
ATGGCAGAGACACATCAACCCTTTTCACTTCAAGATGAAGTTTTTATGCGCAGAGCGATTGAGCTTGCCGCGATTGCTGA
AAGTGAAGGGGAAGTGCCTGTTGGCGCGGTACTGGTCAAAGATGGCGAAGTGATTGCTGAAGGGTGGAATCAATCGATTG
GTCAACATGATGCAACGGCCCATGCAGAAATACAGACCTTACGCCAAGCTGGGCAGAGTCTGGGTAACTATCGTCTGCTT
GACACCACGTTGTACGTCACCTTAGAGCCTTGCCCAATGTGTGCGGGCGCACTGCTGCATAGCCGTGTGAAACGCATTGT
CTTTGGTGCTCCTGATTTGAAAGCGGGGGCGGCAGGGACGGTACTCGATCTGTTTTCTAGTCAAGCGGCATATCACTACG
CAACCATCGAAAAAGGATTGTTAGAGTTGGAATGTCGTGAGCAGTTACAGGCCTTTTTTAAGCGCCGTCGAAAAGAGATC
AAGGCGTTGAAGAAGGAGCAGCAGACTCAAAGTGATCATGAAGATGTGGACGAGGAAGAAAAGTAG

Upstream 100 bases:

>100_bases
TTTATCGCCGAAAGTTCGTTTGCTGGTGGATCATCTGGCACAACATTTATCTGCAGCCGCATAACCGCTATAATCCCCAA
CCCAGACGATAGAGGTAAAC

Downstream 100 bases:

>100_bases
AGAGTAGTAGCAAACATCTGCGTTTCATGGTGCAGATGTTTACTCATCAATTAAGCGTGGTTCACAAGGTGCACAAAAAT
GCGATTGCGAGCGCTAATTT

Product: tRNA-specific adenosine-34 deaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MAETHQPFSLQDEVFMRRAIELAAIAESEGEVPVGAVLVKDGEVIAEGWNQSIGQHDATAHAEIQTLRQAGQSLGNYRLL
DTTLYVTLEPCPMCAGALLHSRVKRIVFGAPDLKAGAAGTVLDLFSSQAAYHYATIEKGLLELECREQLQAFFKRRRKEI
KALKKEQQTQSDHEDVDEEEK

Sequences:

>Translated_181_residues
MAETHQPFSLQDEVFMRRAIELAAIAESEGEVPVGAVLVKDGEVIAEGWNQSIGQHDATAHAEIQTLRQAGQSLGNYRLL
DTTLYVTLEPCPMCAGALLHSRVKRIVFGAPDLKAGAAGTVLDLFSSQAAYHYATIEKGLLELECREQLQAFFKRRRKEI
KALKKEQQTQSDHEDVDEEEK
>Mature_180_residues
AETHQPFSLQDEVFMRRAIELAAIAESEGEVPVGAVLVKDGEVIAEGWNQSIGQHDATAHAEIQTLRQAGQSLGNYRLLD
TTLYVTLEPCPMCAGALLHSRVKRIVFGAPDLKAGAAGTVLDLFSSQAAYHYATIEKGLLELECREQLQAFFKRRRKEIK
ALKKEQQTQSDHEDVDEEEK

Specific function: Deaminates adenosine-34 to inosine in tRNA-Arg2 [H]

COG id: COG0590

COG function: function code FJ; Cytosine/adenosine deaminases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytidine and deoxycytidylate deaminase family [H]

Homologues:

Organism=Homo sapiens, GI124107608, Length=171, Percent_Identity=32.7485380116959, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI145693172, Length=157, Percent_Identity=56.687898089172, Blast_Score=184, Evalue=2e-48,
Organism=Escherichia coli, GI1786616, Length=156, Percent_Identity=35.2564102564103, Blast_Score=74, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554608, Length=106, Percent_Identity=43.3962264150943, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17541138, Length=117, Percent_Identity=36.7521367521368, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555996, Length=79, Percent_Identity=40.5063291139241, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325319, Length=104, Percent_Identity=43.2692307692308, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322425, Length=156, Percent_Identity=33.974358974359, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355527, Length=146, Percent_Identity=34.2465753424658, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016192
- InterPro:   IPR002125
- InterPro:   IPR016193 [H]

Pfam domain/function: PF00383 dCMP_cyt_deam_1 [H]

EC number: NA

Molecular weight: Translated: 20108; Mature: 19977

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00903 CYT_DCMP_DEAMINASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETHQPFSLQDEVFMRRAIELAAIAESEGEVPVGAVLVKDGEVIAEGWNQSIGQHDATA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCHH
HAEIQTLRQAGQSLGNYRLLDTTLYVTLEPCPMCAGALLHSRVKRIVFGAPDLKAGAAGT
HHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
VLDLFSSQAAYHYATIEKGLLELECREQLQAFFKRRRKEIKALKKEQQTQSDHEDVDEEE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
K
C
>Mature Secondary Structure 
AETHQPFSLQDEVFMRRAIELAAIAESEGEVPVGAVLVKDGEVIAEGWNQSIGQHDATA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHCCCCCCHH
HAEIQTLRQAGQSLGNYRLLDTTLYVTLEPCPMCAGALLHSRVKRIVFGAPDLKAGAAGT
HHHHHHHHHHHHHHCCEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
VLDLFSSQAAYHYATIEKGLLELECREQLQAFFKRRRKEIKALKKEQQTQSDHEDVDEEE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]