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The map label for this gene is ribB [H]

Identifier: 27363795

GI number: 27363795

Start: 309281

End: 310390

Strand: Reverse

Name: ribB [H]

Synonym: VV1_0321

Alternate gene names: 27363795

Gene position: 310390-309281 (Counterclockwise)

Preceding gene: 27363796

Following gene: 27363794

Centisome position: 9.46

GC content: 49.73

Gene sequence:

>1110_bases
ATGCCAATCAGTACACCCCAAGAGATCATTGAAGACATTCGTTTGGGCAAAATGGTCATCTTAATGGACGATGAAGACCG
CGAAAACGAAGGTGATTTGATTATGGCGGCGGAGCACATTACGCCAGAAGCCATCAATTTTATGGCCAAATATGGTCGCG
GTTTGATCTGTTTGACTATGACCAAAGAGCGTTGCCAGCGTCTTGGATTACCACCAATGGTGCAAGATAACAACGCGCAA
TACACTACCAACTTCACCGTTTCCATTGAAGCGGCGGAAGGCGTGACAACCGGTATTTCAGCAGCGGATCGTGCTCGTAC
CGTGCAAGCGGCAGTCGCAAAAGAAGCTAAAGCGGCAGACTTGGTGCAACCGGGGCACATTTTCCCATTAGCCGCGCAAG
ATGGTGGCGTGTTGACTCGCGCGGGGCATACTGAGGCGGGCTGCGACCTTGCTCGTTTGGCGGGTTTCGAACCAGCCTCT
GTTATCGTTGAAATTCTGAATGACGATGGCACCATGGCGCGCCGCCCTGATTTGGAAGTGTTTGCTGAGCAGCATGGTCT
CAAACTTGGCACCATTGCCGATTTGATTGAGTATCGAAACAACACGGAAACCACGATTGAGCGCGTTGCTGAGTGCAAGC
TACCCACTGAGTTTGGTGAGTTTGATCTCGTGACCTACCGCGACACCATTGATAATCAAATTCACTTTGCTTTGCGTAAA
GGTACGATGGGTGATCAAACACCACTGGTTCGTGTTCACTTACAAGACACCTTTACCGATTTACTGCGCAGCGATCGCAA
TGCTGAGCGCAGTTGGACGCTTGATAAAGCGATGAAGCGTATTGGCCAAGAAGGGGGCGTTTTGGTGATTCTAGGGAATG
AAGAGTCGCCTGAGCTGCTGATCCATCGCGTCAAAATGTTTGAAGCTCAAGACAAAGAAGAGGCGCCTAAATTGGCTAAG
AAGCAGGGCACTTCACGTCGTGTTGGTGTCGGCTCGCAAATTCTTGCAGACTTAGGTGTGCACGACATGCGTCTACTTTC
TTCCAGCAACAAGAAATACCATGCCCTTGGCGGTTTTGGTTTGAATGTTGTTGAGTATGTGTGTGAATAA

Upstream 100 bases:

>100_bases
TAGTTCACAGAATGTTAGCCCACAGGTTGACACTCGGTAACGATGAATCAGACTAGTGTCTATCAGCGTTTTAGCGCTTA
AATTGACAGGAAATATCGCT

Downstream 100 bases:

>100_bases
CACCACTTCTAAATAAACGCAGCGAAAATAACTCTGCGTTTATTTTTCTCCCCTAATTCGACTTATCTCTTCGCTGTAAG
GCAGTATTTCGTGGCGAAAT

Product: 3,4-dihydroxy-2-butanone 4-phosphate synthase / GTP cyclohydrolase II

Products: NA

Alternate protein names: DHBP synthase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MPISTPQEIIEDIRLGKMVILMDDEDRENEGDLIMAAEHITPEAINFMAKYGRGLICLTMTKERCQRLGLPPMVQDNNAQ
YTTNFTVSIEAAEGVTTGISAADRARTVQAAVAKEAKAADLVQPGHIFPLAAQDGGVLTRAGHTEAGCDLARLAGFEPAS
VIVEILNDDGTMARRPDLEVFAEQHGLKLGTIADLIEYRNNTETTIERVAECKLPTEFGEFDLVTYRDTIDNQIHFALRK
GTMGDQTPLVRVHLQDTFTDLLRSDRNAERSWTLDKAMKRIGQEGGVLVILGNEESPELLIHRVKMFEAQDKEEAPKLAK
KQGTSRRVGVGSQILADLGVHDMRLLSSSNKKYHALGGFGLNVVEYVCE

Sequences:

>Translated_369_residues
MPISTPQEIIEDIRLGKMVILMDDEDRENEGDLIMAAEHITPEAINFMAKYGRGLICLTMTKERCQRLGLPPMVQDNNAQ
YTTNFTVSIEAAEGVTTGISAADRARTVQAAVAKEAKAADLVQPGHIFPLAAQDGGVLTRAGHTEAGCDLARLAGFEPAS
VIVEILNDDGTMARRPDLEVFAEQHGLKLGTIADLIEYRNNTETTIERVAECKLPTEFGEFDLVTYRDTIDNQIHFALRK
GTMGDQTPLVRVHLQDTFTDLLRSDRNAERSWTLDKAMKRIGQEGGVLVILGNEESPELLIHRVKMFEAQDKEEAPKLAK
KQGTSRRVGVGSQILADLGVHDMRLLSSSNKKYHALGGFGLNVVEYVCE
>Mature_368_residues
PISTPQEIIEDIRLGKMVILMDDEDRENEGDLIMAAEHITPEAINFMAKYGRGLICLTMTKERCQRLGLPPMVQDNNAQY
TTNFTVSIEAAEGVTTGISAADRARTVQAAVAKEAKAADLVQPGHIFPLAAQDGGVLTRAGHTEAGCDLARLAGFEPASV
IVEILNDDGTMARRPDLEVFAEQHGLKLGTIADLIEYRNNTETTIERVAECKLPTEFGEFDLVTYRDTIDNQIHFALRKG
TMGDQTPLVRVHLQDTFTDLLRSDRNAERSWTLDKAMKRIGQEGGVLVILGNEESPELLIHRVKMFEAQDKEEAPKLAKK
QGTSRRVGVGSQILADLGVHDMRLLSSSNKKYHALGGFGLNVVEYVCE

Specific function: Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1789420, Length=205, Percent_Identity=54.6341463414634, Blast_Score=222, Evalue=2e-59,
Organism=Escherichia coli, GI1787533, Length=168, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320695, Length=203, Percent_Identity=42.3645320197044, Blast_Score=167, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12 [H]

Molecular weight: Translated: 40660; Mature: 40529

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPISTPQEIIEDIRLGKMVILMDDEDRENEGDLIMAAEHITPEAINFMAKYGRGLICLTM
CCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEE
TKERCQRLGLPPMVQDNNAQYTTNFTVSIEAAEGVTTGISAADRARTVQAAVAKEAKAAD
CHHHHHHCCCCCEEECCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LVQPGHIFPLAAQDGGVLTRAGHTEAGCDLARLAGFEPASVIVEILNDDGTMARRPDLEV
CCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHH
FAEQHGLKLGTIADLIEYRNNTETTIERVAECKLPTEFGEFDLVTYRDTIDNQIHFALRK
HHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEEC
GTMGDQTPLVRVHLQDTFTDLLRSDRNAERSWTLDKAMKRIGQEGGVLVILGNEESPELL
CCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHH
IHRVKMFEAQDKEEAPKLAKKQGTSRRVGVGSQILADLGVHDMRLLSSSNKKYHALGGFG
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCEEEECCCC
LNVVEYVCE
HHHHHHHCC
>Mature Secondary Structure 
PISTPQEIIEDIRLGKMVILMDDEDRENEGDLIMAAEHITPEAINFMAKYGRGLICLTM
CCCCHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEE
TKERCQRLGLPPMVQDNNAQYTTNFTVSIEAAEGVTTGISAADRARTVQAAVAKEAKAAD
CHHHHHHCCCCCEEECCCCEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LVQPGHIFPLAAQDGGVLTRAGHTEAGCDLARLAGFEPASVIVEILNDDGTMARRPDLEV
CCCCCCEEEEECCCCCEEEECCCCCCCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHH
FAEQHGLKLGTIADLIEYRNNTETTIERVAECKLPTEFGEFDLVTYRDTIDNQIHFALRK
HHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEEC
GTMGDQTPLVRVHLQDTFTDLLRSDRNAERSWTLDKAMKRIGQEGGVLVILGNEESPELL
CCCCCCCCEEEEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCHHHH
IHRVKMFEAQDKEEAPKLAKKQGTSRRVGVGSQILADLGVHDMRLLSSSNKKYHALGGFG
HHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCEEEECCCC
LNVVEYVCE
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8144477 [H]