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The map label for this gene is thiI

Identifier: 27363785

GI number: 27363785

Start: 299830

End: 301278

Strand: Reverse

Name: thiI

Synonym: VV1_0310

Alternate gene names: 27363785

Gene position: 301278-299830 (Counterclockwise)

Preceding gene: 326423687

Following gene: 27363784

Centisome position: 9.18

GC content: 47.07

Gene sequence:

>1449_bases
ATGAAATTTATTGTAAAGCCCCATCCAGAAATTTTTGTAAAGAGTGAGTCGGTACGTAAGCGCTTTACCAAGATTCTTGA
GAGCAACATCCGAATCATTATTCAGAATCGCACTGAATCTGTGGCTGTCTTTAACCGTCGCGATCATATCGAAGTGTCGG
CTAACAGCCATCAATATTACCAACAAGTGCTCGAAATCTTGACGACGACACCTGGCATTCAGCAAGTGTTGGAAGTGAAG
CAATCTGGCTTTAAAGATCTGCACGACATCTATGAGCAAGTGTTGGAGCTCAGCCGCGAGCGCATTGAAAACAAAACCTT
TGTGGTGCGTGCTAAGCGCCGTGGTAAGCATGACTTTACCTCAATTGAGCTGGAGCGTTACGTCGGTGGTGGCTTAAATC
AATCGGTCGAGAGCGCCTCGGTCAAACTGCACAACCCAGACATTACCATCAAGATTGAAGTGGTGGATGACAAACTGAAC
CAGATTTTGGCCCATCACAAAGGTCTTGGCGGTTTCCCTCTGGGCACGCAAGAAGATCTGCTGAGCCTGATCTCTGGTGG
TTTTGATTCGGGTGTTTCGAGCTATCTGCACATCAAGCGTGGCTCTAAAGTGCATTACTGTTTCTTCAATCTGGGTGGCC
CTGCGCATGAAATCGGCGTAAAACAAGTGGCTCACTTCTTGTGGAACAAATACGGCTCTTCAGCCAAAGTTCGCTTCATC
TCTGTTGATTTTGAGCCTGTAGTGGCGGAGATTTTAGAGAAGGTTGAAGACGGTCAAATGGGCGTTGTGCTAAAACGTAT
GTTCATGCGTGCAGCTGGCATGGTTGCCGAGAAGTTTGATATTCAAGCTTTGGTAACTGGTGAAGCCCTAGGCCAAGTAT
CAAGCCAAACTTTGACCAACTTACGCCACATTGATGTGGTGACCGATCGTTTGATTCTACGTCCTCTAATCAACTGGGAT
AAAGACGAGATCATTAAAGTTGCACGTGACATCGGCACCGAAGATTTCGCCAAAACCATGCCAGAATACTGTGGCGTGAT
TTCGAAGAAGCCAACCGTGAAAGCAGTCAAAGAGAAGCTCGAAGCGGAAGAAGCCAACTTCAATTTCGATATCTTGGAAC
AAGTGGTACGCAATGCCCGTCAAATGGACATTCGTGATATCGCGAAAGAGTCGGCGCAAGCAGCGCCAGAAGTAGAGCAA
GTTCAAGCGATTGAAGAGCACGCTGTGGTGCTGGATATTCGTAGCCCAGATGAAGAAGACGACAGTCCGCTAGAGATCGA
TGGTGTTGAGGTAAAACACATTCCGTTTTACAAGCTCAGCACGCAATTTGGTGACTTAGACCAGTCGAAAACGTATCTGC
TTTACTGTGCTCGCGGTGTGATGAGCCGTTTGCAAGCTCTCTACCTACAAGAGCAAGGCTTTAACAACGTTAAAGTGTAT
CGCCCATAG

Upstream 100 bases:

>100_bases
TTTTTATGGTTATAATTCGTGTATAATCTTGCGCCCTAAGTCCCACACCTTAGTGCAAGCTGCGGTGTGAATTGATAGCC
AACAGACTGCGAATTGAACT

Downstream 100 bases:

>100_bases
CGGTGATGTGTTAACCCTAATGTCTAAAAATCCGAATGCTCGCATTCGGATTTTTTATTGCCCACGTCCAAGGTTTTATA
AATCTGAACCTAGGTTAAAT

Product: tRNA s(4)U8 sulfurtransferase

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKFIVKPHPEIFVKSESVRKRFTKILESNIRIIIQNRTESVAVFNRRDHIEVSANSHQYYQQVLEILTTTPGIQQVLEVK
QSGFKDLHDIYEQVLELSRERIENKTFVVRAKRRGKHDFTSIELERYVGGGLNQSVESASVKLHNPDITIKIEVVDDKLN
QILAHHKGLGGFPLGTQEDLLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVAHFLWNKYGSSAKVRFI
SVDFEPVVAEILEKVEDGQMGVVLKRMFMRAAGMVAEKFDIQALVTGEALGQVSSQTLTNLRHIDVVTDRLILRPLINWD
KDEIIKVARDIGTEDFAKTMPEYCGVISKKPTVKAVKEKLEAEEANFNFDILEQVVRNARQMDIRDIAKESAQAAPEVEQ
VQAIEEHAVVLDIRSPDEEDDSPLEIDGVEVKHIPFYKLSTQFGDLDQSKTYLLYCARGVMSRLQALYLQEQGFNNVKVY
RP

Sequences:

>Translated_482_residues
MKFIVKPHPEIFVKSESVRKRFTKILESNIRIIIQNRTESVAVFNRRDHIEVSANSHQYYQQVLEILTTTPGIQQVLEVK
QSGFKDLHDIYEQVLELSRERIENKTFVVRAKRRGKHDFTSIELERYVGGGLNQSVESASVKLHNPDITIKIEVVDDKLN
QILAHHKGLGGFPLGTQEDLLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVAHFLWNKYGSSAKVRFI
SVDFEPVVAEILEKVEDGQMGVVLKRMFMRAAGMVAEKFDIQALVTGEALGQVSSQTLTNLRHIDVVTDRLILRPLINWD
KDEIIKVARDIGTEDFAKTMPEYCGVISKKPTVKAVKEKLEAEEANFNFDILEQVVRNARQMDIRDIAKESAQAAPEVEQ
VQAIEEHAVVLDIRSPDEEDDSPLEIDGVEVKHIPFYKLSTQFGDLDQSKTYLLYCARGVMSRLQALYLQEQGFNNVKVY
RP
>Mature_482_residues
MKFIVKPHPEIFVKSESVRKRFTKILESNIRIIIQNRTESVAVFNRRDHIEVSANSHQYYQQVLEILTTTPGIQQVLEVK
QSGFKDLHDIYEQVLELSRERIENKTFVVRAKRRGKHDFTSIELERYVGGGLNQSVESASVKLHNPDITIKIEVVDDKLN
QILAHHKGLGGFPLGTQEDLLSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVAHFLWNKYGSSAKVRFI
SVDFEPVVAEILEKVEDGQMGVVLKRMFMRAAGMVAEKFDIQALVTGEALGQVSSQTLTNLRHIDVVTDRLILRPLINWD
KDEIIKVARDIGTEDFAKTMPEYCGVISKKPTVKAVKEKLEAEEANFNFDILEQVVRNARQMDIRDIAKESAQAAPEVEQ
VQAIEEHAVVLDIRSPDEEDDSPLEIDGVEVKHIPFYKLSTQFGDLDQSKTYLLYCARGVMSRLQALYLQEQGFNNVKVY
RP

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=482, Percent_Identity=64.1078838174274, Blast_Score=639, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_VIBVU (Q8DFA8)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_759313.1
- ProteinModelPortal:   Q8DFA8
- GeneID:   1177272
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_0310
- HOGENOM:   HBG646072
- OMA:   GYPIGTQ
- ProtClustDB:   PRK01269
- BioCyc:   VVUL216895:VV1_0310-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.250.10
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF00581 Rhodanese; PF02568 ThiI; PF02926 THUMP; SSF52821 Rhodanese-like

EC number: =2.8.1.4

Molecular weight: Translated: 54662; Mature: 54662

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50206 RHODANESE_3; PS51165 THUMP

Important sites: ACT_SITE 456-456 BINDING 265-265 BINDING 287-287 BINDING 296-296

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIVKPHPEIFVKSESVRKRFTKILESNIRIIIQNRTESVAVFNRRDHIEVSANSHQYY
CCEEECCCCCEEEECHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCEEEECCCHHHH
QQVLEILTTTPGIQQVLEVKQSGFKDLHDIYEQVLELSRERIENKTFVVRAKRRGKHDFT
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCH
SIELERYVGGGLNQSVESASVKLHNPDITIKIEVVDDKLNQILAHHKGLGGFPLGTQEDL
HEEHHHHHCCCCCCCCCCCEEEEECCCEEEEEEEECHHHHHHHHHHCCCCCCCCCCHHHH
LSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVAHFLWNKYGSSAKVRFI
HHHHHCCCCCCHHHEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
SVDFEPVVAEILEKVEDGQMGVVLKRMFMRAAGMVAEKFDIQALVTGEALGQVSSQTLTN
EECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
LRHIDVVTDRLILRPLINWDKDEIIKVARDIGTEDFAKTMPEYCGVISKKPTVKAVKEKL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EAEEANFNFDILEQVVRNARQMDIRDIAKESAQAAPEVEQVQAIEEHAVVLDIRSPDEED
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCEEEEEECCCCCCC
DSPLEIDGVEVKHIPFYKLSTQFGDLDQSKTYLLYCARGVMSRLQALYLQEQGFNNVKVY
CCCEEECCEEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
RP
CC
>Mature Secondary Structure
MKFIVKPHPEIFVKSESVRKRFTKILESNIRIIIQNRTESVAVFNRRDHIEVSANSHQYY
CCEEECCCCCEEEECHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCEEEECCCHHHH
QQVLEILTTTPGIQQVLEVKQSGFKDLHDIYEQVLELSRERIENKTFVVRAKRRGKHDFT
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCH
SIELERYVGGGLNQSVESASVKLHNPDITIKIEVVDDKLNQILAHHKGLGGFPLGTQEDL
HEEHHHHHCCCCCCCCCCCEEEEECCCEEEEEEEECHHHHHHHHHHCCCCCCCCCCHHHH
LSLISGGFDSGVSSYLHIKRGSKVHYCFFNLGGPAHEIGVKQVAHFLWNKYGSSAKVRFI
HHHHHCCCCCCHHHEEEEECCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
SVDFEPVVAEILEKVEDGQMGVVLKRMFMRAAGMVAEKFDIQALVTGEALGQVSSQTLTN
EECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHH
LRHIDVVTDRLILRPLINWDKDEIIKVARDIGTEDFAKTMPEYCGVISKKPTVKAVKEKL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
EAEEANFNFDILEQVVRNARQMDIRDIAKESAQAAPEVEQVQAIEEHAVVLDIRSPDEED
HHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHCEEEEEECCCCCCC
DSPLEIDGVEVKHIPFYKLSTQFGDLDQSKTYLLYCARGVMSRLQALYLQEQGFNNVKVY
CCCEEECCEEEEECCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
RP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA