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The map label for this gene is gltX [H]

Identifier: 27363718

GI number: 27363718

Start: 226537

End: 227961

Strand: Reverse

Name: gltX [H]

Synonym: VV1_0236

Alternate gene names: 27363718

Gene position: 227961-226537 (Counterclockwise)

Preceding gene: 27363719

Following gene: 27363715

Centisome position: 6.95

GC content: 47.72

Gene sequence:

>1425_bases
ATGACGGTTAAAACTCGTTTTGCTCCTAGCCCAACTGGCTACCTACACGTTGGTGGTGCTCGTACAGCACTTTACTCTTG
GTTATACGCGAAAAGCCAAGGTGGTGAGTTCGTTCTACGTATCGAAGATACTGACTTAGAGCGCAATTCTCAGGAAGCGG
TAGATGCCATTCTTGAAGGTATGCAGTGGCTAGGTTTGGAATGGAACGAAGGTCCTTATTTCCAAACTCAGCGTTTTGAC
CGTTACAACGAGATGGTTGACAAGCTGCTTGCGGAAGACAAAGCGTACAAATGTTACGCATCAAAAGAGCTGCTTGATGA
AGTTCGTGCAGAGCAAGAAGCCAACAAAGAAATGCCTCGCTATGACGCTGAGCATCCAAAAATCAAAGCGGCAAATGAAG
CGGCAAAAGATGGCGATCCATGCGTGATTCGTTTCCGTAACCCGAAAGAGGGTAGCGTGGTATTCGAAGATCAAATCCGT
GGTCGTATCGAAATCAGCAACAGCCAAATGGATGATCTCATCATCCGTCGCACTGACGGCTCACCAACCTACAACTTCTG
TGTGGTGGTTGACGACTGGGATATGGGCATTACGCACGTTATTCGTGGCGAAGACCATATCAACAACACGCCTCGTCAAA
TCAACATCTATGAAGCATTAGGCGCGCCAGTCCCTACGTTTGCGCATTGTGCCATGATTCTAGGTGATGACGGTGCGAAA
CTGTCTAAGCGTCATGGTGCGGTATCAGTGATGCAATACCGCGATATGGGTTACTTGCCAGTGGCATTGAACAACTACCT
CGTGCGCCTTGGTTGGTCTCACGGCGACCAAGAAATTTTCTCTCAAGAAGAGATGATCAACCTATTTAGCTTGAATGCTA
TCTCAAAGTCAGCGTCTGCGTTTAATACCGACAAGCTGCTTTGGTTGAATAACCACTACATCAAAACATCGGATCCTGAG
TATGTGGCAGAACACCTGCAATGGCACTTGGATCAAAAGGGCATCAAGACAGAAAATGGTCCAGCCATCACTGACGTGAT
CAAACTGGTTGGTGAGCGTTGCAATACGTTGGTTGAGTTGGCCGATCAAATTGGTTACTTCTACCAAGATTTTGACGCAT
TTGATGCAGATGCAGCGAAAAAACACTTACGTGGTGTGGCGAAGCAGCCGCTAGAAGTCGCACTGGCTAAAGTGGAAGCA
CTTACTGAGTGGACAACAGAAAACCTACACCAAGTGATTGCCGATGTGTGTACTGAGCTTGAAATCGGTATGGGCAAAAT
CGGTATGCCTCTGCGTGTTGCTGTTACTGGTGGCGGTCAGTCTCCTTCTGTGGATGCGGTGATGCAGCTCATCGGTAAAG
AGCGTGTTGTTGCGCGTATTAAGATGGCGTTGGCGTTTATTGCAGAGCGTGAAGCAAACGCATAA

Upstream 100 bases:

>100_bases
TGGTTGTAGATTCGTTCGACGATGGTAAAATGTCGCCACTTTTGTCCTTGAACTCGCAGTGCTTGAAATAGAGGCTGTAT
TTTATCCCTAAGGAATTCTG

Downstream 100 bases:

>100_bases
TTGGCCAGCGAGAATAACAAACCGCAGCTCTGTGCTGCGGTTTTTTTTGGACTTTGTTTGAGTGCGAAAAAGAGCACGCG
TTATTTGAGACCGATACTGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTVKTRFAPSPTGYLHVGGARTALYSWLYAKSQGGEFVLRIEDTDLERNSQEAVDAILEGMQWLGLEWNEGPYFQTQRFD
RYNEMVDKLLAEDKAYKCYASKELLDEVRAEQEANKEMPRYDAEHPKIKAANEAAKDGDPCVIRFRNPKEGSVVFEDQIR
GRIEISNSQMDDLIIRRTDGSPTYNFCVVVDDWDMGITHVIRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAK
LSKRHGAVSVMQYRDMGYLPVALNNYLVRLGWSHGDQEIFSQEEMINLFSLNAISKSASAFNTDKLLWLNNHYIKTSDPE
YVAEHLQWHLDQKGIKTENGPAITDVIKLVGERCNTLVELADQIGYFYQDFDAFDADAAKKHLRGVAKQPLEVALAKVEA
LTEWTTENLHQVIADVCTELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVARIKMALAFIAEREANA

Sequences:

>Translated_474_residues
MTVKTRFAPSPTGYLHVGGARTALYSWLYAKSQGGEFVLRIEDTDLERNSQEAVDAILEGMQWLGLEWNEGPYFQTQRFD
RYNEMVDKLLAEDKAYKCYASKELLDEVRAEQEANKEMPRYDAEHPKIKAANEAAKDGDPCVIRFRNPKEGSVVFEDQIR
GRIEISNSQMDDLIIRRTDGSPTYNFCVVVDDWDMGITHVIRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAK
LSKRHGAVSVMQYRDMGYLPVALNNYLVRLGWSHGDQEIFSQEEMINLFSLNAISKSASAFNTDKLLWLNNHYIKTSDPE
YVAEHLQWHLDQKGIKTENGPAITDVIKLVGERCNTLVELADQIGYFYQDFDAFDADAAKKHLRGVAKQPLEVALAKVEA
LTEWTTENLHQVIADVCTELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVARIKMALAFIAEREANA
>Mature_473_residues
TVKTRFAPSPTGYLHVGGARTALYSWLYAKSQGGEFVLRIEDTDLERNSQEAVDAILEGMQWLGLEWNEGPYFQTQRFDR
YNEMVDKLLAEDKAYKCYASKELLDEVRAEQEANKEMPRYDAEHPKIKAANEAAKDGDPCVIRFRNPKEGSVVFEDQIRG
RIEISNSQMDDLIIRRTDGSPTYNFCVVVDDWDMGITHVIRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAKL
SKRHGAVSVMQYRDMGYLPVALNNYLVRLGWSHGDQEIFSQEEMINLFSLNAISKSASAFNTDKLLWLNNHYIKTSDPEY
VAEHLQWHLDQKGIKTENGPAITDVIKLVGERCNTLVELADQIGYFYQDFDAFDADAAKKHLRGVAKQPLEVALAKVEAL
TEWTTENLHQVIADVCTELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVARIKMALAFIAEREANA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=493, Percent_Identity=30.2231237322515, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI62241042, Length=239, Percent_Identity=28.0334728033473, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=69.8513800424628, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=246, Percent_Identity=33.3333333333333, Blast_Score=135, Evalue=8e-33,
Organism=Escherichia coli, GI1786895, Length=208, Percent_Identity=25.9615384615385, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=488, Percent_Identity=30.327868852459, Blast_Score=205, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6324540, Length=497, Percent_Identity=30.9859154929577, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=217, Percent_Identity=29.9539170506912, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=213, Percent_Identity=26.2910798122066, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665321, Length=489, Percent_Identity=30.4703476482618, Blast_Score=231, Evalue=8e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 53240; Mature: 53109

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTRFAPSPTGYLHVGGARTALYSWLYAKSQGGEFVLRIEDTDLERNSQEAVDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
MQWLGLEWNEGPYFQTQRFDRYNEMVDKLLAEDKAYKCYASKELLDEVRAEQEANKEMPR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
YDAEHPKIKAANEAAKDGDPCVIRFRNPKEGSVVFEDQIRGRIEISNSQMDDLIIRRTDG
CCCCCCCEECCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCCCCEEEEECCC
SPTYNFCVVVDDWDMGITHVIRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAK
CCCEEEEEEEECCCCCCEEEECCCHHCCCCCCEEEEHHHHCCCCCHHHHEEEEECCCCCH
LSKRHGAVSVMQYRDMGYLPVALNNYLVRLGWSHGDQEIFSQEEMINLFSLNAISKSASA
HHHHCCCEEEEEECCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
FNTDKLLWLNNHYIKTSDPEYVAEHLQWHLDQKGIKTENGPAITDVIKLVGERCNTLVEL
CCCCEEEEEECCEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ADQIGYFYQDFDAFDADAAKKHLRGVAKQPLEVALAKVEALTEWTTENLHQVIADVCTEL
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVARIKMALAFIAEREANA
HCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVKTRFAPSPTGYLHVGGARTALYSWLYAKSQGGEFVLRIEDTDLERNSQEAVDAILEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
MQWLGLEWNEGPYFQTQRFDRYNEMVDKLLAEDKAYKCYASKELLDEVRAEQEANKEMPR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
YDAEHPKIKAANEAAKDGDPCVIRFRNPKEGSVVFEDQIRGRIEISNSQMDDLIIRRTDG
CCCCCCCEECCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCCCCEEEEECCC
SPTYNFCVVVDDWDMGITHVIRGEDHINNTPRQINIYEALGAPVPTFAHCAMILGDDGAK
CCCEEEEEEEECCCCCCEEEECCCHHCCCCCCEEEEHHHHCCCCCHHHHEEEEECCCCCH
LSKRHGAVSVMQYRDMGYLPVALNNYLVRLGWSHGDQEIFSQEEMINLFSLNAISKSASA
HHHHCCCEEEEEECCCCCEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
FNTDKLLWLNNHYIKTSDPEYVAEHLQWHLDQKGIKTENGPAITDVIKLVGERCNTLVEL
CCCCEEEEEECCEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ADQIGYFYQDFDAFDADAAKKHLRGVAKQPLEVALAKVEALTEWTTENLHQVIADVCTEL
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVARIKMALAFIAEREANA
HCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA