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The map label for this gene is aroK

Identifier: 23502877

GI number: 23502877

Start: 1954951

End: 1955553

Strand: Reverse

Name: aroK

Synonym: BR2029

Alternate gene names: 23502877

Gene position: 1955553-1954951 (Counterclockwise)

Preceding gene: 23502883

Following gene: 23502876

Centisome position: 92.78

GC content: 58.21

Gene sequence:

>603_bases
ATGAGCGGTACGAACAAACAAACAAATCTTCACAGGCAGACGGAAACGATCCGCCAGCTGCTCGGCAGTAAAGTGGTTGT
TCTCGTTGGCCTGATGGGAGCCGGAAAATCCACCATCGGCCGCAAGGTGGCGAACATGCTCAACCTGCCGTTCAAGGATG
CGGATACGGAAATTGAAACTGTTTCGCGGATGACTGTTGCCGAACTGTTCGAGGCCTATGGGGAAGTGGAATTTCGCGAT
CTCGAACGTCGCGTGATCCTGCGCCTTCTCGATGACGGCCCCATGGTGCTGGCGACCGGCGGCGGCGCCTATATGAACGC
CGAAACCCGCGCGGCGATTGCCGAAGCGGGGATTTCCATCTGGATTAATGCCGATCTCGACGTCTTGATGGAGCGCGTTT
CGCGCCGCCAGAACCGTCCCCTTTTGAGAAACAGCGATCCGCGCGGCGTCATGCAGCGGCTGATGGACGAGCGCTACCCG
GTTTATGCGCTGGCCGAGCTGCATCTGATGACGCGCGATGAGAAAAAGGAAGTGATTGCCGCAGAACTGATCGAGGTTCT
GGCGGCGCATCTGGAAAAGGAACAGGCCGCTTCCGCCGGATGA

Upstream 100 bases:

>100_bases
TGATCGCGTGAAAAAGCCGCTCCGAGCGCGACAAAAACCATGAAAGTAATTGACGCCGCTTCGTTTTTCATGTGGAACCG
GGGTGGAACGGAAGAGGCTG

Downstream 100 bases:

>100_bases
CGGCCGAAACCTGAAGCGGTCCACGGGCCGTTTTCTTTATTTGTTTCGTGTGTCCGGTCTTTCGTCCACCGGATACCAGC
AGGAGCTGACCAATGAATGC

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSGTNKQTNLHRQTETIRQLLGSKVVVLVGLMGAGKSTIGRKVANMLNLPFKDADTEIETVSRMTVAELFEAYGEVEFRD
LERRVILRLLDDGPMVLATGGGAYMNAETRAAIAEAGISIWINADLDVLMERVSRRQNRPLLRNSDPRGVMQRLMDERYP
VYALAELHLMTRDEKKEVIAAELIEVLAAHLEKEQAASAG

Sequences:

>Translated_200_residues
MSGTNKQTNLHRQTETIRQLLGSKVVVLVGLMGAGKSTIGRKVANMLNLPFKDADTEIETVSRMTVAELFEAYGEVEFRD
LERRVILRLLDDGPMVLATGGGAYMNAETRAAIAEAGISIWINADLDVLMERVSRRQNRPLLRNSDPRGVMQRLMDERYP
VYALAELHLMTRDEKKEVIAAELIEVLAAHLEKEQAASAG
>Mature_199_residues
SGTNKQTNLHRQTETIRQLLGSKVVVLVGLMGAGKSTIGRKVANMLNLPFKDADTEIETVSRMTVAELFEAYGEVEFRDL
ERRVILRLLDDGPMVLATGGGAYMNAETRAAIAEAGISIWINADLDVLMERVSRRQNRPLLRNSDPRGVMQRLMDERYPV
YALAELHLMTRDEKKEVIAAELIEVLAAHLEKEQAASAG

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=167, Percent_Identity=38.9221556886228, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786587, Length=162, Percent_Identity=29.0123456790123, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_BRUA1 (B2S8S9)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935857.1
- ProteinModelPortal:   B2S8S9
- SMR:   B2S8S9
- GeneID:   6327003
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I19010
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK13946
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 22235; Mature: 22104

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 55-55 BINDING 79-79 BINDING 101-101 BINDING 139-139 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTNKQTNLHRQTETIRQLLGSKVVVLVGLMGAGKSTIGRKVANMLNLPFKDADTEIET
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
VSRMTVAELFEAYGEVEFRDLERRVILRLLDDGPMVLATGGGAYMNAETRAAIAEAGISI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEECHHHHHHHHHCCEEE
WINADLDVLMERVSRRQNRPLLRNSDPRGVMQRLMDERYPVYALAELHLMTRDEKKEVIA
EECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHH
AELIEVLAAHLEKEQAASAG
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGTNKQTNLHRQTETIRQLLGSKVVVLVGLMGAGKSTIGRKVANMLNLPFKDADTEIET
CCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
VSRMTVAELFEAYGEVEFRDLERRVILRLLDDGPMVLATGGGAYMNAETRAAIAEAGISI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEECHHHHHHHHHCCEEE
WINADLDVLMERVSRRQNRPLLRNSDPRGVMQRLMDERYPVYALAELHLMTRDEKKEVIA
EECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHH
AELIEVLAAHLEKEQAASAG
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA