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The map label for this gene is mdeA [H]

Identifier: 23502814

GI number: 23502814

Start: 1894785

End: 1896068

Strand: Reverse

Name: mdeA [H]

Synonym: BR1965

Alternate gene names: 23502814

Gene position: 1896068-1894785 (Counterclockwise)

Preceding gene: 23502815

Following gene: 23502813

Centisome position: 89.96

GC content: 58.96

Gene sequence:

>1284_bases
ATGACTGCGCCGCGCCCATCGAAGATCCGTTTGGGCAATCATCTTCTTCATCCAGAAACGCAGATGCTGAATTATGGCTA
CGACCCGGAGCTTTCCGAGGGCGCGGTCAAGCCGCCTGTGTTTCTCACATCGACCTTCGTTTTCAAGACAGCGGAAGACG
GACGCGATTTCTTCGATTTCGTTTCGGGGCGCAAGGAGCCGCCGGCCGGTGTCGGGGCGGGGCTTGTCTATTCACGCTTC
AACCACCCGAACAGCGAGATTGTGGAGGACCGTCTGGCGGTCTATGAGGGGGCGGAAAGTGCAGCCCTTTTCTCGTCCGG
CATGTCCGCCATTGCCACCACGCTTTTCGCTTTTGTGCGCCCGGGCGATGTCATCCTGCATTCGCAGCCACTTTATGGCG
GCACGGAAACGCTTCTGGCGAAGACCTTCTTCAATTTTGGCGTGGAGGCTGTCGCTTTTGCGGATGGTGTTCACGAGGCC
ACCATCGAGAAAGCAGCCGAAGAGGCGCTGGCGAAAGGTCGTGTTTCGGTCATTTTGATCGAAACCCCGGCCAATCCGAC
CAATAGTATTGTCGATGTCGCAGCGGTTCGCCGCGTGGCGGAGAAGATTGAAGCGCGGCAGGGCAGCCGCCCGGTCATCG
CCTGCGACAATACCCTGCTCGGGCCGGTGTTCCAGAAGCCTCTTGATCACGGGGCCGATCTCTCGGTCTATTCGCTGACC
AAATATGTCGGCGGTCATTCCGATCTTATTGCAGGGGCGGTGCTGGGCGCAAAATCCGTTGTGCGCCAGGTAAAGGCCCT
GCGCGGAGCAATCGGTACACAGCTCGATCCGCATTCATGCTGGATGCTTGGCCGCTCCCTGGAAACGCTGGGCCTGCGCA
TGGAGCGGGCTGACAGCAATGCCCGCGCCATTGCTGAATTCCTGCGTAATCACCCCAAGGTGGAAAAGCTGCATTATCTG
CCTTTTGCCGATGAGCGTTCCGATATCGCAGCCTTGTTCAAGCGCCAATGTACCGGCGCTGGCTCGACCTTTTCCTTCGA
TATAAAGGGCGGGCAGGCTGCTGCTTTCCGTTTCCTGAATGCGCTGCAAATCCTGAAACTCGCGGTAAGCCTCGGTGGCA
CCGAATCGCTTGCGAGCCATCCCGCGGCGATGACGCATTCAGGTGTTCCGGTCGATGTGCGTGAGCGGATCGGCGTGCTG
GAATCGACCATCCGCCTTTCCATAGGTATCGAGCATCCCGGCGACCTGATCGCCGATCTGGCACAGGCTTTGGATGCGGC
CTGA

Upstream 100 bases:

>100_bases
AAGATTCCGTTTCACCTCTGGTAATGTCCTCCTGACCGCCATCCGGCGCGTCCGATCATTCGGACGCTCCGGTCGCGGTA
ATTGTTTTGAGGAGGCAACC

Downstream 100 bases:

>100_bases
TACCAAAATCTAATGCGCTTGACGGTTCCGTTGCAAAAATCCGGTTGTGACGGAACGCGGCTCATCTGCAAATGATGGTC
ATCCGATTGGGTCCATCGCC

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAPRPSKIRLGNHLLHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDFVSGRKEPPAGVGAGLVYSRF
NHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVRPGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEA
TIEKAAEEALAKGRVSVILIETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLT
KYVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSNARAIAEFLRNHPKVEKLHYL
PFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLNALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVL
ESTIRLSIGIEHPGDLIADLAQALDAA

Sequences:

>Translated_427_residues
MTAPRPSKIRLGNHLLHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDFVSGRKEPPAGVGAGLVYSRF
NHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVRPGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEA
TIEKAAEEALAKGRVSVILIETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLT
KYVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSNARAIAEFLRNHPKVEKLHYL
PFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLNALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVL
ESTIRLSIGIEHPGDLIADLAQALDAA
>Mature_426_residues
TAPRPSKIRLGNHLLHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDFVSGRKEPPAGVGAGLVYSRFN
HPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVRPGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEAT
IEKAAEEALAKGRVSVILIETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLTK
YVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSNARAIAEFLRNHPKVEKLHYLP
FADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLNALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVLE
STIRLSIGIEHPGDLIADLAQALDAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=418, Percent_Identity=35.4066985645933, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI299473759, Length=418, Percent_Identity=32.7751196172249, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI25453487, Length=418, Percent_Identity=32.0574162679426, Blast_Score=175, Evalue=9e-44,
Organism=Escherichia coli, GI1790375, Length=405, Percent_Identity=36.5432098765432, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1789383, Length=414, Percent_Identity=27.7777777777778, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17538055, Length=416, Percent_Identity=33.8942307692308, Blast_Score=202, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI17560056, Length=416, Percent_Identity=34.375, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=422, Percent_Identity=32.4644549763033, Blast_Score=198, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6319307, Length=402, Percent_Identity=34.0796019900498, Blast_Score=196, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=425, Percent_Identity=27.2941176470588, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=28.0092592592593, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=413, Percent_Identity=29.0556900726392, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=28.0898876404494, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922554, Length=410, Percent_Identity=34.1463414634146, Blast_Score=225, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45700; Mature: 45568

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPRPSKIRLGNHLLHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDF
CCCCCCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHH
VSGRKEPPAGVGAGLVYSRFNHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVR
HCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
PGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEATIEKAAEEALAKGRVSVILI
CCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEE
ETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLT
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHH
KYVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCH
ARAIAEFLRNHPKVEKLHYLPFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLN
HHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
ALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVLESTIRLSIGIEHPGDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCHHHHHHH
AQALDAA
HHHHCCC
>Mature Secondary Structure 
TAPRPSKIRLGNHLLHPETQMLNYGYDPELSEGAVKPPVFLTSTFVFKTAEDGRDFFDF
CCCCCCEEECCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCHHHHHH
VSGRKEPPAGVGAGLVYSRFNHPNSEIVEDRLAVYEGAESAALFSSGMSAIATTLFAFVR
HCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
PGDVILHSQPLYGGTETLLAKTFFNFGVEAVAFADGVHEATIEKAAEEALAKGRVSVILI
CCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEE
ETPANPTNSIVDVAAVRRVAEKIEARQGSRPVIACDNTLLGPVFQKPLDHGADLSVYSLT
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHH
KYVGGHSDLIAGAVLGAKSVVRQVKALRGAIGTQLDPHSCWMLGRSLETLGLRMERADSN
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHCCCH
ARAIAEFLRNHPKVEKLHYLPFADERSDIAALFKRQCTGAGSTFSFDIKGGQAAAFRFLN
HHHHHHHHHCCCCHHHEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
ALQILKLAVSLGGTESLASHPAAMTHSGVPVDVRERIGVLESTIRLSIGIEHPGDLIADL
HHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCHHHHHHH
AQALDAA
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]