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The map label for this gene is ansB [H]

Identifier: 23502811

GI number: 23502811

Start: 1891725

End: 1892714

Strand: Reverse

Name: ansB [H]

Synonym: BR1962

Alternate gene names: 23502811

Gene position: 1892714-1891725 (Counterclockwise)

Preceding gene: 23502813

Following gene: 23502810

Centisome position: 89.8

GC content: 56.26

Gene sequence:

>990_bases
ATGTCTTTAATTTCTATCGGCCCCCTCGGGGGTACGATTGCGATGGTTGAGGGGGGAAGCGGAGGCGGTGTTGTGCCGAC
CCTGACCGCGGATGCACTCATTGCCGCTGTGCCGCAGATTAGAAATTATGCGCAGATCAAGGCCCAATCTCTTTTTCAGC
TTCCCAGCCCCTCGCTAAAATTTCGGCACGGCCTTGAAGTGATAGGCTGGGCTGAAAACGAGGTGAAATCGGGAGCGGAC
GGCATAGTGCTGACGCAGGGAACCGATACGCTTGAGGAAATGGCATTCCTTCTGGATTTATTGTGGGAACATCCGCAGCC
CCTCGTACTGACCGGCGCCATGCGCTCGCCGCGCGCGCCGGGTGCTGACGGTCCGGCCAATCTGGTGGCGGCCGTATTGA
CAGCCGCGAGCAGGCTTAGCCGGGAACGCGGTGTCCTTGTTGCAATGAACGATACAATCCACGCGGCACGTTGGATAACA
AAATCTCATGCGCTCAGTGTCCAGACATTCGTATCGTCGGACAGCGGGCCGTTGGGATATGTGTATGAGGAGACGCCGGT
TTACATCAACACCCTTTCGCGGTTGCCAAAAATTGACCGTACGCAGTTCAACGCCGATGTGAAGATCGGCTTTTACGAAT
CGCTGCTGGATGGTGACGCGCAGATGCTGCGAAGCATGTTTGAGAGCGGCAGATATGATGGAATCGTCGTCGCCTGTTTT
GGAGCAGGACATGTGTCGGGCGACGAAGCCGACATTATCGAGCGCTATGCGTCCCGCATTCCGGTCGTCGTAGCCAGCCG
GACCTATGGCGGAAGGACCGCCACGAAGACTTATGGGTTCCATGGCTCCGAGATTGATCTTCAGACGAAAGGCGCTATGC
TCGCAGGCTGGTTATCCCCCCGCAAGGCGCGGTTATTGCTGTGGGCACTACTGGCAGCGGGTAAAAATCTGGAAGAAACA
AGAGCTAACTTCCTGCTCTATAACCCATAA

Upstream 100 bases:

>100_bases
TCGGGTTTCAAAAGCGGATTCACATAGACCTCCCCGTGTTGATTCCTATACAAAACTCAAATATGGATAATGCATTATGC
ATAATTTTAGGGAGTTGGAA

Downstream 100 bases:

>100_bases
CTGCCGATCGAGATTCCGCATCCATGGTAAAAGAGCCACGTTTTAGCAGTCGTCTGGAAAAGCCCCTCACGACTGCGCAA
TATGTCGCCGATTATCTGCG

Product: asparaginase family protein

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSLISIGPLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLKFRHGLEVIGWAENEVKSGAD
GIVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAPGADGPANLVAAVLTAASRLSRERGVLVAMNDTIHAARWIT
KSHALSVQTFVSSDSGPLGYVYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVACF
GAGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSPRKARLLLWALLAAGKNLEET
RANFLLYNP

Sequences:

>Translated_329_residues
MSLISIGPLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLKFRHGLEVIGWAENEVKSGAD
GIVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAPGADGPANLVAAVLTAASRLSRERGVLVAMNDTIHAARWIT
KSHALSVQTFVSSDSGPLGYVYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVACF
GAGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSPRKARLLLWALLAAGKNLEET
RANFLLYNP
>Mature_328_residues
SLISIGPLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLKFRHGLEVIGWAENEVKSGADG
IVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAPGADGPANLVAAVLTAASRLSRERGVLVAMNDTIHAARWITK
SHALSVQTFVSSDSGPLGYVYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVACFG
AGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSPRKARLLLWALLAAGKNLEETR
ANFLLYNP

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=302, Percent_Identity=34.4370860927152, Blast_Score=152, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323189, Length=337, Percent_Identity=31.4540059347181, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323187, Length=337, Percent_Identity=31.4540059347181, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323186, Length=337, Percent_Identity=31.4540059347181, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323184, Length=337, Percent_Identity=31.4540059347181, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320527, Length=327, Percent_Identity=30.8868501529052, Blast_Score=120, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35303; Mature: 35172

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLISIGPLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLK
CCEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FRHGLEVIGWAENEVKSGADGIVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAP
HHCCCEEEEECCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
GADGPANLVAAVLTAASRLSRERGVLVAMNDTIHAARWITKSHALSVQTFVSSDSGPLGY
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCEEEEEEECCCCCCEEE
VYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVACF
EEECCCEEEHHHHHCCCCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCEEEEEE
GAGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSP
ECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCEEEEEECCCEEEECCCC
RKARLLLWALLAAGKNLEETRANFLLYNP
HHHHHHHHHHHHCCCCHHHCCCCEEEECC
>Mature Secondary Structure 
SLISIGPLGGTIAMVEGGSGGGVVPTLTADALIAAVPQIRNYAQIKAQSLFQLPSPSLK
CEEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
FRHGLEVIGWAENEVKSGADGIVLTQGTDTLEEMAFLLDLLWEHPQPLVLTGAMRSPRAP
HHCCCEEEEECCHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCC
GADGPANLVAAVLTAASRLSRERGVLVAMNDTIHAARWITKSHALSVQTFVSSDSGPLGY
CCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCEEEEEEECCCCCCEEE
VYEETPVYINTLSRLPKIDRTQFNADVKIGFYESLLDGDAQMLRSMFESGRYDGIVVACF
EEECCCEEEHHHHHCCCCCCCCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCCCEEEEEE
GAGHVSGDEADIIERYASRIPVVVASRTYGGRTATKTYGFHGSEIDLQTKGAMLAGWLSP
ECCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCCCCEEEEEECCCEEEECCCC
RKARLLLWALLAAGKNLEETRANFLLYNP
HHHHHHHHHHHHCCCCHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]