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The map label for this gene is leuC

Identifier: 23502757

GI number: 23502757

Start: 1840512

End: 1841921

Strand: Reverse

Name: leuC

Synonym: BR1906

Alternate gene names: 23502757

Gene position: 1841921-1840512 (Counterclockwise)

Preceding gene: 23502758

Following gene: 23502756

Centisome position: 87.39

GC content: 60.35

Gene sequence:

>1410_bases
ATGAGCGCGCCGCGTACACTTTATGACAAGATTTGGGATGACCATGTAGTGGATCAGCAGGAAGACGGCACCTGCCTCCT
TTATATTGATCGCCATCTTGTGCATGAAGTGACGAGCCCGCAGGCCTTTGAGGGGCTGCGCATGGCAGGCCGCCCGGTGC
GCCACCCGGAAAAGACGCTTGCCGTGGTGGACCATAACGTTCCGACGTCGCCCGACCGCATCAATGGCATCCAGAACGAG
GAAAGCCGCATCCAGGTCGAGGCACTGGCCAGGAATGCCGCCGATTTCGGCGTCGAATATTATTCCGAACGCGACAAGCG
CCAGGGCATCGTGCATATCGTCGGGCCAGAGCAGGGCTTCACCCTGCCGGGCATGACCATCGTTTGCGGTGACAGCCACA
CTTCCACCCACGGCGCTTTCGGTGCGCTTGCACATGGCATCGGCACATCGGAGGTGGAGCATGTGCTCGCCACCCAGACG
CTGATCCAGAAAAAAGCCAAGAATATGCTGGTGCGCGTTGACGGCAAATTGCCGGCGGGCGTGACCGCCAAGGATATTGT
TCTGGCGATCATTGGCGAGATCGGCACGGCAGGCGGCACCGGCTATGTCATCGAATATGCGGGCGAAGCCATTCGCTCGC
TGTCGATGGAAGGCCGCATGACGATCTGCAACATGTCGATCGAGGGCGGCGCGCGTGCCGGTCTTATCGCTCCCGACGAG
ACGACGTTTGAATATATCAAGGGCCGCCCGCGTGCTCCGCAGGGCGAAACGCTGGAACAGGCGATCAATTACTGGAAGAC
GCTGCATTCGGATGAAGGGGCGCATTTCGACAAGATCGTGACGCTCGACGCCGGCAGCCTGCCGCCCATCGTTTCCTGGG
GTTCTTCGCCGGAAGACGTCGTTTCCGTCACGGGGGTCGTGCCCAATCCGGACGATATCGCCGACGAAACCAAGCGTGCC
TCCAAGTGGCGTGCGCTCGACTATATGGGCCTGAAGCCCGGCACGAAAATCACGGATATCGCGGTTGATCGCGTCTTCAT
CGGTTCGTGCACCAATGGCCGCATCGAAGACCTTCGCGCTGCCGCCAAGGTCGTGGAAGGCAAGAAGGTTGCCCCGACCG
TCAATGCCATGATCGTTCCGGGTTCGGGTCTCGTAAAGGAACAGGCGGAAGCCGAAGGCCTGCACAAGATTTTCATTGAA
GCCGGTTTCGACTGGCGCGAGCCGGGCTGCTCCATGTGCCTTGCGATGAACGATGACCGGCTGAAGCCGGGCGAGCGTTG
CGCTTCGACCTCGAACCGCAATTTCGAGGGCCGTCAGGGCTTCAAGGGTCGTACGCATCTGGTATCGCCAGCAATGGCTG
CGGCGGCTGCAATTGCCGGTCACTTCGTCGATATTCGCGCGTGGAAGTGA

Upstream 100 bases:

>100_bases
TTACTGCGACACCCTGTTGCGGTTTCTGTTTCTTGACGAAAAGCGGTGGCAGGTGCATATGACAGCCTGGAATTAAGAAC
CGTTCAAAGGAACCCTCGCA

Downstream 100 bases:

>100_bases
GCACAACCCCGCATAAAGTTTGAAAAGACCGCCGGTTGATCCCGGCGGTCTTTTCGTTTTCCGTCACGACAGATCGGTCG
CACATATATCTTAACGAGGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNE
ESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDE
TTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRA
SKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIE
AGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK

Sequences:

>Translated_469_residues
MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNE
ESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDE
TTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRA
SKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIE
AGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
>Mature_468_residues
SAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRPVRHPEKTLAVVDHNVPTSPDRINGIQNEE
SRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQKKAKNMLVRVDGKLPAGVTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDET
TFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDVVSVTGVVPNPDDIADETKRAS
KWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRAAAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEA
GFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=382, Percent_Identity=28.0104712041885, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI8659555, Length=385, Percent_Identity=27.012987012987, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI41352693, Length=384, Percent_Identity=26.3020833333333, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=67.741935483871, Blast_Score=642, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=361, Percent_Identity=28.808864265928, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081781, Length=348, Percent_Identity=25, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=382, Percent_Identity=30.1047120418848, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI32564738, Length=382, Percent_Identity=30.1047120418848, Blast_Score=137, Evalue=9e-33,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=30.6666666666667, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=407, Percent_Identity=28.5012285012285, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=472, Percent_Identity=65.0423728813559, Blast_Score=619, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6320440, Length=426, Percent_Identity=25.5868544600939, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=473, Percent_Identity=27.906976744186, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6322261, Length=487, Percent_Identity=26.4887063655031, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281365315, Length=481, Percent_Identity=25.3638253638254, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI17864292, Length=481, Percent_Identity=25.3638253638254, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=26.7015706806283, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI28571643, Length=362, Percent_Identity=27.0718232044199, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24645686, Length=381, Percent_Identity=30.1837270341207, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137564, Length=379, Percent_Identity=28.2321899736148, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_BRUA1 (B2S860)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935743.1
- ProteinModelPortal:   B2S860
- GeneID:   6326887
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I17870
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50712; Mature: 50581

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRPVRHPEKTL
CCCCHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCEE
AVVDHNVPTSPDRINGIQNEESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGF
EEEECCCCCCHHHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPAG
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
VTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDE
CCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
TTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCHHHH
VSVTGVVPNPDDIADETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRA
EEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCEEEHEEEEEEEEECCCCCHHHHHHH
AAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEAGFDWREPGCSMCLAMNDDR
HHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
CCCHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure 
SAPRTLYDKIWDDHVVDQQEDGTCLLYIDRHLVHEVTSPQAFEGLRMAGRPVRHPEKTL
CCCHHHHHHHCCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCEE
AVVDHNVPTSPDRINGIQNEESRIQVEALARNAADFGVEYYSERDKRQGIVHIVGPEQGF
EEEECCCCCCHHHHCCCCCCCHHEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGKLPAG
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCC
VTAKDIVLAIIGEIGTAGGTGYVIEYAGEAIRSLSMEGRMTICNMSIEGGARAGLIAPDE
CCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCC
TTFEYIKGRPRAPQGETLEQAINYWKTLHSDEGAHFDKIVTLDAGSLPPIVSWGSSPEDV
HHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCHHHH
VSVTGVVPNPDDIADETKRASKWRALDYMGLKPGTKITDIAVDRVFIGSCTNGRIEDLRA
EEEECCCCCCHHHHHHHHHHHHHHHEEECCCCCCCEEEHEEEEEEEEECCCCCHHHHHHH
AAKVVEGKKVAPTVNAMIVPGSGLVKEQAEAEGLHKIFIEAGFDWREPGCSMCLAMNDDR
HHHHHCCCCCCCCCCEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIRAWK
CCCHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA