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The map label for this gene is priA [H]

Identifier: 23502657

GI number: 23502657

Start: 1731839

End: 1734073

Strand: Reverse

Name: priA [H]

Synonym: BR1804

Alternate gene names: 23502657

Gene position: 1734073-1731839 (Counterclockwise)

Preceding gene: 23502659

Following gene: 23502656

Centisome position: 82.27

GC content: 62.46

Gene sequence:

>2235_bases
ATGTCGAAAGATTCGCACGATATTGCAAACCCCATGTCTGGTCTGTTTCCGGAACTCGCGCCACGGGTGGTTCCCGTGCT
CGTACCCATGCCCGCGGAACGGCCTTATTCTTATATGGTTCCGCCGGGCATGAACGTCCAGCCCGGATCGATCGTGCGCG
TGCCACTCGGGCCGCGGGAGGTGGCGGGGATTGTCTGCGAGGGCGAGACCGATGCGGTCGATTCCAGAAAATTGCGGGAG
ATTTCAGAAGTTTTCAATTGCCCGCTAGTGGGGCCTGAGATGCTGCGCTTCATGCGCTGGGCAGCCGATTATACGCTTTC
GCCGCCCGGCATGGTGGCGCGCATGGTTTTGCGCGTGCCTGCCGCCTTCGACCCCGAACCGGCGGTGCCGGGGCTTCGCT
ATCGTGCCGGCGAGCCGGAGCGCATGCCAGAGCGCATGACTGCGGCTCGCGCCCGCGTGATGGAGCTTGCCCGCGACGGG
CTTGCCTGGACACGCTCCGGCCTTGCCCATGCGGCGGGCGTATCGCTGACGGTCGTGGATGGCTTGAAGGCGCAAGGCAT
TTTTGAAGAAATCATGCTGCCGCCGCCAGCCGTGGTTGCAAAGCCCGATACGGATTATGCGCGCGCCACGCTTTCGCAGG
ACCAGCAGGCAGCCGCCGAAATGCTGTCGGAAGCCGTCGAAGCCGGTGGTTTTTCGGTTTCTCTGCTCGATGGTGTGACG
GGGTCGGGCAAGACGGAGGTTTATTTTGAAGCGGTCGCCAAGGCGCTGGATGCGGGCAGGCAGGTGCTGATTCTCCTGCC
GGAAATCGCGCTGACCCAGCAGTTTCTCGACCGCTTTCACGACCGCTTTGGCGCAAAGCCCGCCGAATGGCATTCCGATC
TTGCGCCGCGCACCCGCGAGCGCGTCTGGCGGCAGGTTGCGGAGGGAACAGTGCGGGTTGTGGCAGGTGCGCGCTCAGCA
CTTTTCCTGCCCTTCAAGGAACTGGGGCTGATCGTCGTCGATGAAGAACATGATCCGGCTTACAAACAGGAAGACCGCGT
TTTCTACAATGCGCGCGATATGGCCGTGGTGCGTGGCCATATTGGCAGCTTTCCGGTCGTGCTCGCCTCGGCCACGCCGT
CCATCGAAAGTCAGGTCAATGCGGAGCAGGGCCGCTACAAGCGAATCAAGCTCTATGGCCGCTATGCTGAAGCAGCACTT
CCCGATCTTAAAACCATCGACATGCGCCGTTCGCCGCCGCCGCCGGGCCGGTTTCTGTCGCCTGCCCTGACGGAAGCTGT
CGGCAAGACAGTGGAGCGCGGCGAGCAGGCGCTTTTGTTCCTCAACCGGCGCGGCTATGCGCCATTGACGCTCTGCCGGG
TCTGCGGCCACCGCTTTCAATGTCCGCAATGTTCAAGCTGGCTGGTGGAACATCGTTTTCGCGGCCAGTTGATGTGCCAC
CAGTGCGGCTATCATGAGCCGGTGCCGCAAGCCTGCCCGGAATGCGGCACGCTGGATCATCTGGTTGCCTGCGGCCCCGG
CGTGGAGCGCATCGCGGAGGAAGTGGCGGCAACCTTCAATGACGCGCGAATCATCGTTCTTTCGTCTGATATGGCGGGTG
GCGTGAAGCGGCTGCGGCTGGAACTTGACGCTATCGCAAAAGGCGAAACGGATATCGTCATCGGCACGCAGCTTGTCGCC
AAAGGACACAATTTTCCCAACATGACACTGGTGGGCGTGGTGGATGCCGATCTGGGGCTTGCCAATGGCGATCCGCGCGC
GGCCGAACGCACGTTCCAGTTGCTCAATCAGGTCACGGGCCGCGCGGGGCGAACGGGGCGCAAGAGCCTCGGTCTCATTC
AGACTTATCAGCCTGACCATCCCGTGATGCGGGCTATCGTCAGCGGCGATGCGGACGCCTTTTACGCCCGCGAAATCGAG
GAGCGCGAGCGCAGCAATCTGCCGCCTTTCGGGCGGCTTGCGGCGCTCATCATCTCCGCTGATAACCGGCCCGACGCTGA
AAATCATGCGCGCGCGCTTCGCCGCTGTGCGCCCTCGTCGCGTGAAATTTCCGTGCTGGGACCAGCCGAAGCGCCGCTTG
CGCTGGTGCGCGGGCGCCATCGGTTCCGCATTCTCGTGCACGGGACAAAACGCGCGGATATTCAAGGATTTATCCGCGCG
CTTCTGGCGGCTGCGCCAAAGGAAAAAGGCTCGATCAGGGTGCAGGTGGATATAGATCCGCAAAGCTTTCTCTGA

Upstream 100 bases:

>100_bases
CCTCCATAATGAGCGCGGGTAGATTTGAGGAATTTAAAACCATTTAATTCCCGGAAGGTGGCTTTTCTTTAGCGCAAAGC
CGCGCCAAGGTCACGCGATC

Downstream 100 bases:

>100_bases
CATGTAAAGGCGTGGAAGATTGATGGAAATTCCGCTATAGGGCAAGAAACGACGAGGGTAATTGCGATGGAATTCTATCT
TCCTGCCACGACCGGAGAAT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 744; Mature: 743

Protein sequence:

>744_residues
MSKDSHDIANPMSGLFPELAPRVVPVLVPMPAERPYSYMVPPGMNVQPGSIVRVPLGPREVAGIVCEGETDAVDSRKLRE
ISEVFNCPLVGPEMLRFMRWAADYTLSPPGMVARMVLRVPAAFDPEPAVPGLRYRAGEPERMPERMTAARARVMELARDG
LAWTRSGLAHAAGVSLTVVDGLKAQGIFEEIMLPPPAVVAKPDTDYARATLSQDQQAAAEMLSEAVEAGGFSVSLLDGVT
GSGKTEVYFEAVAKALDAGRQVLILLPEIALTQQFLDRFHDRFGAKPAEWHSDLAPRTRERVWRQVAEGTVRVVAGARSA
LFLPFKELGLIVVDEEHDPAYKQEDRVFYNARDMAVVRGHIGSFPVVLASATPSIESQVNAEQGRYKRIKLYGRYAEAAL
PDLKTIDMRRSPPPPGRFLSPALTEAVGKTVERGEQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRGQLMCH
QCGYHEPVPQACPECGTLDHLVACGPGVERIAEEVAATFNDARIIVLSSDMAGGVKRLRLELDAIAKGETDIVIGTQLVA
KGHNFPNMTLVGVVDADLGLANGDPRAAERTFQLLNQVTGRAGRTGRKSLGLIQTYQPDHPVMRAIVSGDADAFYAREIE
ERERSNLPPFGRLAALIISADNRPDAENHARALRRCAPSSREISVLGPAEAPLALVRGRHRFRILVHGTKRADIQGFIRA
LLAAAPKEKGSIRVQVDIDPQSFL

Sequences:

>Translated_744_residues
MSKDSHDIANPMSGLFPELAPRVVPVLVPMPAERPYSYMVPPGMNVQPGSIVRVPLGPREVAGIVCEGETDAVDSRKLRE
ISEVFNCPLVGPEMLRFMRWAADYTLSPPGMVARMVLRVPAAFDPEPAVPGLRYRAGEPERMPERMTAARARVMELARDG
LAWTRSGLAHAAGVSLTVVDGLKAQGIFEEIMLPPPAVVAKPDTDYARATLSQDQQAAAEMLSEAVEAGGFSVSLLDGVT
GSGKTEVYFEAVAKALDAGRQVLILLPEIALTQQFLDRFHDRFGAKPAEWHSDLAPRTRERVWRQVAEGTVRVVAGARSA
LFLPFKELGLIVVDEEHDPAYKQEDRVFYNARDMAVVRGHIGSFPVVLASATPSIESQVNAEQGRYKRIKLYGRYAEAAL
PDLKTIDMRRSPPPPGRFLSPALTEAVGKTVERGEQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRGQLMCH
QCGYHEPVPQACPECGTLDHLVACGPGVERIAEEVAATFNDARIIVLSSDMAGGVKRLRLELDAIAKGETDIVIGTQLVA
KGHNFPNMTLVGVVDADLGLANGDPRAAERTFQLLNQVTGRAGRTGRKSLGLIQTYQPDHPVMRAIVSGDADAFYAREIE
ERERSNLPPFGRLAALIISADNRPDAENHARALRRCAPSSREISVLGPAEAPLALVRGRHRFRILVHGTKRADIQGFIRA
LLAAAPKEKGSIRVQVDIDPQSFL
>Mature_743_residues
SKDSHDIANPMSGLFPELAPRVVPVLVPMPAERPYSYMVPPGMNVQPGSIVRVPLGPREVAGIVCEGETDAVDSRKLREI
SEVFNCPLVGPEMLRFMRWAADYTLSPPGMVARMVLRVPAAFDPEPAVPGLRYRAGEPERMPERMTAARARVMELARDGL
AWTRSGLAHAAGVSLTVVDGLKAQGIFEEIMLPPPAVVAKPDTDYARATLSQDQQAAAEMLSEAVEAGGFSVSLLDGVTG
SGKTEVYFEAVAKALDAGRQVLILLPEIALTQQFLDRFHDRFGAKPAEWHSDLAPRTRERVWRQVAEGTVRVVAGARSAL
FLPFKELGLIVVDEEHDPAYKQEDRVFYNARDMAVVRGHIGSFPVVLASATPSIESQVNAEQGRYKRIKLYGRYAEAALP
DLKTIDMRRSPPPPGRFLSPALTEAVGKTVERGEQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRGQLMCHQ
CGYHEPVPQACPECGTLDHLVACGPGVERIAEEVAATFNDARIIVLSSDMAGGVKRLRLELDAIAKGETDIVIGTQLVAK
GHNFPNMTLVGVVDADLGLANGDPRAAERTFQLLNQVTGRAGRTGRKSLGLIQTYQPDHPVMRAIVSGDADAFYAREIEE
RERSNLPPFGRLAALIISADNRPDAENHARALRRCAPSSREISVLGPAEAPLALVRGRHRFRILVHGTKRADIQGFIRAL
LAAAPKEKGSIRVQVDIDPQSFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=736, Percent_Identity=34.1032608695652, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1787357, Length=118, Percent_Identity=36.4406779661017, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD [H]

EC number: NA

Molecular weight: Translated: 81346; Mature: 81214

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDSHDIANPMSGLFPELAPRVVPVLVPMPAERPYSYMVPPGMNVQPGSIVRVPLGPRE
CCCCCCHHHCHHHHHHHHHHHCCEEEEECCCCCCCCCEECCCCCCCCCCCEEEECCCHHH
VAGIVCEGETDAVDSRKLREISEVFNCPLVGPEMLRFMRWAADYTLSPPGMVARMVLRVP
HEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
AAFDPEPAVPGLRYRAGEPERMPERMTAARARVMELARDGLAWTRSGLAHAAGVSLTVVD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHCCCEEEEEC
GLKAQGIFEEIMLPPPAVVAKPDTDYARATLSQDQQAAAEMLSEAVEAGGFSVSLLDGVT
CCCCCCHHHHHCCCCCCEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCC
GSGKTEVYFEAVAKALDAGRQVLILLPEIALTQQFLDRFHDRFGAKPAEWHSDLAPRTRE
CCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHH
RVWRQVAEGTVRVVAGARSALFLPFKELGLIVVDEEHDPAYKQEDRVFYNARDMAVVRGH
HHHHHHHCCCEEEEECCCCEEEEEHHHCCEEEEECCCCCCCCCCCCEEECCCCCEEECCC
IGSFPVVLASATPSIESQVNAEQGRYKRIKLYGRYAEAALPDLKTIDMRRSPPPPGRFLS
CCCCCEEEEECCCCHHHHCCCCCCCEEEEEEEECHHHHHCCCCCEECCCCCCCCCCCCCC
PALTEAVGKTVERGEQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRGQLMCH
HHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHH
QCGYHEPVPQACPECGTLDHLVACGPGVERIAEEVAATFNDARIIVLSSDMAGGVKRLRL
HCCCCCCCHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ELDAIAKGETDIVIGTQLVAKGHNFPNMTLVGVVDADLGLANGDPRAAERTFQLLNQVTG
HHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHC
RAGRTGRKSLGLIQTYQPDHPVMRAIVSGDADAFYAREIEERERSNLPPFGRLAALIISA
CCCCCCCHHCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHEEEEEEC
DNRPDAENHARALRRCAPSSREISVLGPAEAPLALVRGRHRFRILVHGTKRADIQGFIRA
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHH
LLAAAPKEKGSIRVQVDIDPQSFL
HHHHCCCCCCCEEEEEECCHHHCC
>Mature Secondary Structure 
SKDSHDIANPMSGLFPELAPRVVPVLVPMPAERPYSYMVPPGMNVQPGSIVRVPLGPRE
CCCCCHHHCHHHHHHHHHHHCCEEEEECCCCCCCCCEECCCCCCCCCCCEEEECCCHHH
VAGIVCEGETDAVDSRKLREISEVFNCPLVGPEMLRFMRWAADYTLSPPGMVARMVLRVP
HEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
AAFDPEPAVPGLRYRAGEPERMPERMTAARARVMELARDGLAWTRSGLAHAAGVSLTVVD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHCCCEEEEEC
GLKAQGIFEEIMLPPPAVVAKPDTDYARATLSQDQQAAAEMLSEAVEAGGFSVSLLDGVT
CCCCCCHHHHHCCCCCCEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEECCCC
GSGKTEVYFEAVAKALDAGRQVLILLPEIALTQQFLDRFHDRFGAKPAEWHSDLAPRTRE
CCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHH
RVWRQVAEGTVRVVAGARSALFLPFKELGLIVVDEEHDPAYKQEDRVFYNARDMAVVRGH
HHHHHHHCCCEEEEECCCCEEEEEHHHCCEEEEECCCCCCCCCCCCEEECCCCCEEECCC
IGSFPVVLASATPSIESQVNAEQGRYKRIKLYGRYAEAALPDLKTIDMRRSPPPPGRFLS
CCCCCEEEEECCCCHHHHCCCCCCCEEEEEEEECHHHHHCCCCCEECCCCCCCCCCCCCC
PALTEAVGKTVERGEQALLFLNRRGYAPLTLCRVCGHRFQCPQCSSWLVEHRFRGQLMCH
HHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHH
QCGYHEPVPQACPECGTLDHLVACGPGVERIAEEVAATFNDARIIVLSSDMAGGVKRLRL
HCCCCCCCHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ELDAIAKGETDIVIGTQLVAKGHNFPNMTLVGVVDADLGLANGDPRAAERTFQLLNQVTG
HHHHHCCCCCEEEEEHHHHCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHC
RAGRTGRKSLGLIQTYQPDHPVMRAIVSGDADAFYAREIEERERSNLPPFGRLAALIISA
CCCCCCCHHCCEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHEEEEEEC
DNRPDAENHARALRRCAPSSREISVLGPAEAPLALVRGRHRFRILVHGTKRADIQGFIRA
CCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHCCCCEEEEEEECCCCHHHHHHHHH
LLAAAPKEKGSIRVQVDIDPQSFL
HHHHCCCCCCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2861810 [H]