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The map label for this gene is pyk [H]

Identifier: 23502605

GI number: 23502605

Start: 1682359

End: 1683795

Strand: Reverse

Name: pyk [H]

Synonym: BR1748

Alternate gene names: 23502605

Gene position: 1683795-1682359 (Counterclockwise)

Preceding gene: 23502606

Following gene: 23502599

Centisome position: 79.88

GC content: 59.15

Gene sequence:

>1437_bases
ATGAAGCGCAACCGCAAGGTCAAGATTCTCGCCACGCTAGGTCCAGCCTCTGGCGAGGAAGCCGTCATCCGCAAGCTCTT
TGAAGCAGGAGCGGATGTCTTCCGCATCAATATGAGTCACGCCGACCATGACCTGATGCGAACCTTGGTCAAGCGCATTC
GCAATGTAGAAAAGGAACTGGGACGCCCCATCGGCATTCTGGCCGATCTTCAGGGGCCGAAGCTGCGCGTTGGCAAGTTC
AAGGATGGCAAGGTCGATCTCGTTCCCGGCCAGACCTTCACCCTCGACAATAACGAGGCGCTGGGCGATGAAACCCGCGT
TTTCCTGCCGCATCCTGAAATTCTGGAAGCTGTCGAGCCGGGCCATCGCCTGTTGATCGATGACGGAAAACTGCATCTGG
TGGCTGAAGCCTGCGACGGCAAGTCGATCCGCTGCAAGGTTGTTTCCGGCACCCGCATTTCCGACCGCAAGGGGGTCAGC
CTTCCCGACACGACGCTCGGCGTTGGCGCGCTGACGGAGAAAGACCGCTGCGATCTTGATGCCGTGCTGAAGGAAGAGAT
CGATTGGGTGGCGCTTTCCTTTATCCAGCGCCCGGAAGATCTGGCCGAAGTGCGCAAGGTTTCGCGCGGCAAGGTCGGCC
TCATGTCCAAGATCGAAAAGCCGCAGGCTGTTACCCGTCTTGATGAAATTATCGAGCTTTCCGACGCCTTGATGGTTGCC
CGCGGCGATCTCGGTGTTGAAGTGCCGCTTGAAAACGTTCCCGGCATTCAAAAGCAGATTACCCGCAAGGCGCGTCGCGC
CGGCAAGCCGGTCGTTGTCGCAACCCAGATGCTGGAATCGATGATTACCGCGCCGGTGCCCACCCGCGCTGAAGTGTCCG
ACGTGGCGACTGCGGTGTTTGAAGGTGCGGATGCAATCATGCTTTCGGCGGAATCTGCTTCCGGCCAATATCCGGTCGAA
GCTGTTGCAACCATGAACCGCATCGCCGAGCAGGTGGAGCGCGAGCCGACCTATTCGACCATCATTGACGCACAGCGCGC
CATGCCGGAGCCGACTGGCGCCGACGCTATCTCGCTCGCCGCGCGTCAGATTGCTGAAACGTTGAAACTCTCGGCCATCG
TGACCTATACGGCTTCCGGCACGACCGGCCTGCGCGCTGCCCGCGAGCGTCCGCGCACACCCATTATCGCGCTGTCGCCC
GTTGTTGATACCGCCCGTAAACTGTCCCTCGTATGGGGCCTGCATTGCGTCGTCACAGATGATGCCCATGATCTTGAAGA
CATGGTCAATCATGCCTGCGAAATCGTGTTCCATGAGGAATTCGGCAAAGCTGGCGATCGCGTCATCATCACGGCAGGCG
TTCCCTTCGGAACGCCGGGTGCAACCAATATGCTGCGTATCGCCTATATAGGCGCTGACGGAAAATCCGGCATTTAG

Upstream 100 bases:

>100_bases
GTTTTCAGGAATACGATACCGGGGAACAATCAAGCTGGATGGTTCAGCTCACCGATGAAACGCCGACAGACAACCCCACT
GTTACAGGAACGAATAATCA

Downstream 100 bases:

>100_bases
AGCGCATCCCGAAAAGTGTGAAACGGTTTTCGGAAAAGATGCGCGTCAAAACAAAGGATTAGAGCGCCGATCTGATTCAA
TCAGATCGAAATGCGCTCTA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKRNRKVKILATLGPASGEEAVIRKLFEAGADVFRINMSHADHDLMRTLVKRIRNVEKELGRPIGILADLQGPKLRVGKF
KDGKVDLVPGQTFTLDNNEALGDETRVFLPHPEILEAVEPGHRLLIDDGKLHLVAEACDGKSIRCKVVSGTRISDRKGVS
LPDTTLGVGALTEKDRCDLDAVLKEEIDWVALSFIQRPEDLAEVRKVSRGKVGLMSKIEKPQAVTRLDEIIELSDALMVA
RGDLGVEVPLENVPGIQKQITRKARRAGKPVVVATQMLESMITAPVPTRAEVSDVATAVFEGADAIMLSAESASGQYPVE
AVATMNRIAEQVEREPTYSTIIDAQRAMPEPTGADAISLAARQIAETLKLSAIVTYTASGTTGLRAARERPRTPIIALSP
VVDTARKLSLVWGLHCVVTDDAHDLEDMVNHACEIVFHEEFGKAGDRVIITAGVPFGTPGATNMLRIAYIGADGKSGI

Sequences:

>Translated_478_residues
MKRNRKVKILATLGPASGEEAVIRKLFEAGADVFRINMSHADHDLMRTLVKRIRNVEKELGRPIGILADLQGPKLRVGKF
KDGKVDLVPGQTFTLDNNEALGDETRVFLPHPEILEAVEPGHRLLIDDGKLHLVAEACDGKSIRCKVVSGTRISDRKGVS
LPDTTLGVGALTEKDRCDLDAVLKEEIDWVALSFIQRPEDLAEVRKVSRGKVGLMSKIEKPQAVTRLDEIIELSDALMVA
RGDLGVEVPLENVPGIQKQITRKARRAGKPVVVATQMLESMITAPVPTRAEVSDVATAVFEGADAIMLSAESASGQYPVE
AVATMNRIAEQVEREPTYSTIIDAQRAMPEPTGADAISLAARQIAETLKLSAIVTYTASGTTGLRAARERPRTPIIALSP
VVDTARKLSLVWGLHCVVTDDAHDLEDMVNHACEIVFHEEFGKAGDRVIITAGVPFGTPGATNMLRIAYIGADGKSGI
>Mature_478_residues
MKRNRKVKILATLGPASGEEAVIRKLFEAGADVFRINMSHADHDLMRTLVKRIRNVEKELGRPIGILADLQGPKLRVGKF
KDGKVDLVPGQTFTLDNNEALGDETRVFLPHPEILEAVEPGHRLLIDDGKLHLVAEACDGKSIRCKVVSGTRISDRKGVS
LPDTTLGVGALTEKDRCDLDAVLKEEIDWVALSFIQRPEDLAEVRKVSRGKVGLMSKIEKPQAVTRLDEIIELSDALMVA
RGDLGVEVPLENVPGIQKQITRKARRAGKPVVVATQMLESMITAPVPTRAEVSDVATAVFEGADAIMLSAESASGQYPVE
AVATMNRIAEQVEREPTYSTIIDAQRAMPEPTGADAISLAARQIAETLKLSAIVTYTASGTTGLRAARERPRTPIIALSP
VVDTARKLSLVWGLHCVVTDDAHDLEDMVNHACEIVFHEEFGKAGDRVIITAGVPFGTPGATNMLRIAYIGADGKSGI

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=486, Percent_Identity=37.6543209876543, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=37.4485596707819, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=37.4485596707819, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=36.2525458248472, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=36.2525458248472, Blast_Score=265, Evalue=8e-71,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=36.2369337979094, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=36.2369337979094, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=32.0675105485232, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=32.0675105485232, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=31.7757009345794, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=39.3305439330544, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1787965, Length=462, Percent_Identity=35.4978354978355, Blast_Score=273, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17544584, Length=482, Percent_Identity=35.2697095435685, Blast_Score=278, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=35.4771784232365, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI71984406, Length=482, Percent_Identity=35.4771784232365, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17506829, Length=482, Percent_Identity=35.4771784232365, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=482, Percent_Identity=35.4771784232365, Blast_Score=252, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6319279, Length=488, Percent_Identity=35.2459016393443, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=33.4702258726899, Blast_Score=237, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24648964, Length=484, Percent_Identity=36.5702479338843, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI28571814, Length=484, Percent_Identity=36.5702479338843, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24648966, Length=402, Percent_Identity=35.0746268656716, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=31.9018404907975, Blast_Score=238, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24646914, Length=256, Percent_Identity=39.0625, Blast_Score=168, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51682; Mature: 51682

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNRKVKILATLGPASGEEAVIRKLFEAGADVFRINMSHADHDLMRTLVKRIRNVEKEL
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
GRPIGILADLQGPKLRVGKFKDGKVDLVPGQTFTLDNNEALGDETRVFLPHPEILEAVEP
CCCEEEEEECCCCCEEECCCCCCCEEEECCCEEEECCCCCCCCCCEEEECCHHHHHHCCC
GHRLLIDDGKLHLVAEACDGKSIRCKVVSGTRISDRKGVSLPDTTLGVGALTEKDRCDLD
CCEEEEECCCEEEEEEECCCCCEEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCHH
AVLKEEIDWVALSFIQRPEDLAEVRKVSRGKVGLMSKIEKPQAVTRLDEIIELSDALMVA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHEEE
RGDLGVEVPLENVPGIQKQITRKARRAGKPVVVATQMLESMITAPVPTRAEVSDVATAVF
ECCCCEECCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EGADAIMLSAESASGQYPVEAVATMNRIAEQVEREPTYSTIIDAQRAMPEPTGADAISLA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHH
ARQIAETLKLSAIVTYTASGTTGLRAARERPRTPIIALSPVVDTARKLSLVWGLHCVVTD
HHHHHHHHHEEEEEEEECCCCHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHEEEEEEEC
DAHDLEDMVNHACEIVFHEEFGKAGDRVIITAGVPFGTPGATNMLRIAYIGADGKSGI
CHHHHHHHHHHHHEEEEHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MKRNRKVKILATLGPASGEEAVIRKLFEAGADVFRINMSHADHDLMRTLVKRIRNVEKEL
CCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
GRPIGILADLQGPKLRVGKFKDGKVDLVPGQTFTLDNNEALGDETRVFLPHPEILEAVEP
CCCEEEEEECCCCCEEECCCCCCCEEEECCCEEEECCCCCCCCCCEEEECCHHHHHHCCC
GHRLLIDDGKLHLVAEACDGKSIRCKVVSGTRISDRKGVSLPDTTLGVGALTEKDRCDLD
CCEEEEECCCEEEEEEECCCCCEEEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCHH
AVLKEEIDWVALSFIQRPEDLAEVRKVSRGKVGLMSKIEKPQAVTRLDEIIELSDALMVA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHEEE
RGDLGVEVPLENVPGIQKQITRKARRAGKPVVVATQMLESMITAPVPTRAEVSDVATAVF
ECCCCEECCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHH
EGADAIMLSAESASGQYPVEAVATMNRIAEQVEREPTYSTIIDAQRAMPEPTGADAISLA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHH
ARQIAETLKLSAIVTYTASGTTGLRAARERPRTPIIALSPVVDTARKLSLVWGLHCVVTD
HHHHHHHHHEEEEEEEECCCCHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHEEEEEEEC
DAHDLEDMVNHACEIVFHEEFGKAGDRVIITAGVPFGTPGATNMLRIAYIGADGKSGI
CHHHHHHHHHHHHEEEEHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA