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The map label for this gene is purA

Identifier: 23502541

GI number: 23502541

Start: 1626066

End: 1627355

Strand: Reverse

Name: purA

Synonym: BR1683

Alternate gene names: 23502541

Gene position: 1627355-1626066 (Counterclockwise)

Preceding gene: 23502542

Following gene: 23502540

Centisome position: 77.21

GC content: 60.93

Gene sequence:

>1290_bases
ATGGCAAATGTGGTTGTCGTCGGGTCGCAATGGGGCGACGAGGGCAAGGGAAAGATCGTTGACTGGCTGTCCGAGCGTGC
CGACGTGATCGTGCGCTACCAGGGCGGTCATAACGCAGGTCATACGCTTGTCATCGACGGTGTCAGCTACAAGCTTTCGC
TGCTGCCATCCGGCCTCGTGCGCGGCAAGTTGAGCGTTATCGGCAATGGCGTCGTGGTCGATCCGCACCATTTCGTCGCC
GAAGTTGAAAAGCTGCGCGGGCAGGGCATCGATGTGACGCCGGATGTGCTGCGCGTTGCGGAAAACGCACCGCTTATCCT
TTCGATCCATCGTGAACTCGACGCCATGCGCGAAGGCTCCAATTCAGGCCTCAAGATCGGCACGACCAAGCGCGGCATCG
GCCCCGCTTACGAAGACAAGGTTGGCCGCCGCGCCATCCGCGTGATCGATCTGACCGAGCCGGAAACATTGCGGCCGAAG
GTGGAGCGCCTGCTTGCGCACCATAATTCGCTGCGCCGCGGCATGGGCCTTGAAGAAATCGCCGTTGAAACCATCCTCAC
CGAACTGACTTCGGTTGCCGACCAGATCCTGCCCTATATCGATCAGGTCTGGCGTGTCCTGGACGAGCGCCGCAAGGCTG
GCGCCCGTATCCTGTTTGAAGGTGCGCAGGGCGCGCTGCTCGACAATGATCACGGCACCTATCCGTTCGTGACCTCTTCC
AACACGGTTGCCGGTCAGGCCGCTGCCGGTTCCGGCCTCGGCCCCACGGCTATCGGCTATGTGCTTGGCATCACCAAGGC
CTATACAACCCGCGTTGGCGAAGGTCCGTTCCCGACCGAGCTCAATGACGAGATCGGCGAATTCCTCGGCACCAAGGGCC
ATGAATTCGGCGTGGTGACGGGCCGCAAGCGCCGTTGCGGCTGGTTCGATGCCGTCATCGTGCGCCAGACAGTGCGCACT
TCCGGCATCAACGGTATTGCACTTACCAAGCTCGATGTTCTGGATGGTCTGGAAGAAATCAAGATTTGCGTCGCCTATGA
ACTCGACGGCAAGCGCATCGATTATCTGCCGTCTTCCATGGGCGCGCAGGCCCGCGTGAAGCCGATCTACGAAACCTTGC
CGGGCTGGTCTGAAACAACCGCCGGTGCGCGTTCGTGGAACGATCTTCCCGCGCAGGCGGTCAAATATGTCCGCCATATC
GAAGAGCTGATCGGTGCGCCGGTGGCGATGCTCTCCACAAGCCCCGAGCGCGAGGATACGATCCTCGTAACCGATCCGTT
CCACGACTGA

Upstream 100 bases:

>100_bases
CTGTATAGAGACGAGGGATCAGCACCCTGTAGCCTCCGCTGGCAATGACCGGGCCGCTTTGATCCTTGGATTTTCAAATA
GAAGCCAAGGAGTTTCTTCA

Downstream 100 bases:

>100_bases
TTGTTTGATGATCCAGCCCGGCATGTCCGGGCTGGTTCGTTTCTGCCGCAAGCATTTCCAGCAAAAGTGTGAAACGGAGA
CGCTGGAAATGCTCTGGTTT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLVRGKLSVIGNGVVVDPHHFVA
EVEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPK
VERLLAHHNSLRRGMGLEEIAVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSS
NTVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVTGRKRRCGWFDAVIVRQTVRT
SGINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSMGAQARVKPIYETLPGWSETTAGARSWNDLPAQAVKYVRHI
EELIGAPVAMLSTSPEREDTILVTDPFHD

Sequences:

>Translated_429_residues
MANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLVRGKLSVIGNGVVVDPHHFVA
EVEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPK
VERLLAHHNSLRRGMGLEEIAVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSS
NTVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVTGRKRRCGWFDAVIVRQTVRT
SGINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSMGAQARVKPIYETLPGWSETTAGARSWNDLPAQAVKYVRHI
EELIGAPVAMLSTSPEREDTILVTDPFHD
>Mature_428_residues
ANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLVRGKLSVIGNGVVVDPHHFVAE
VEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGSNSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKV
ERLLAHHNSLRRGMGLEEIAVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSSN
TVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVTGRKRRCGWFDAVIVRQTVRTS
GINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSMGAQARVKPIYETLPGWSETTAGARSWNDLPAQAVKYVRHIE
ELIGAPVAMLSTSPEREDTILVTDPFHD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=423, Percent_Identity=45.6264775413712, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=42.5882352941177, Blast_Score=337, Evalue=2e-92,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=43.9086294416244, Blast_Score=314, Evalue=9e-86,
Organism=Escherichia coli, GI1790620, Length=428, Percent_Identity=52.803738317757, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=42.5233644859813, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=42.5233644859813, Blast_Score=326, Evalue=2e-89,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=41.958041958042, Blast_Score=331, Evalue=1e-91,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=42.8909952606635, Blast_Score=335, Evalue=3e-92,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_BRUA2 (Q2YQI5)

Other databases:

- EMBL:   AM040264
- EMBL:   L43054
- RefSeq:   YP_415045.1
- ProteinModelPortal:   Q2YQI5
- SMR:   Q2YQI5
- STRING:   Q2YQI5
- GeneID:   3788872
- GenomeReviews:   AM040264_GR
- KEGG:   bmf:BAB1_1695
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   BMEL359391:BAB1_1695-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46562; Mature: 46430

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLV
CCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEEEEECCCHHH
RGKLSVIGNGVVVDPHHFVAEVEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGS
HCCCEEECCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCC
NSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKVERLLAHHNSLRRGMGLEEI
CCCEEECCCCCCCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
AVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEECC
NTVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEE
GRKRRCGWFDAVIVRQTVRTSGINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSM
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHEEEEEEEECCCCEEECCCCCC
GAQARVKPIYETLPGWSETTAGARSWNDLPAQAVKYVRHIEELIGAPVAMLSTSPEREDT
CCCCEECHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCE
ILVTDPFHD
EEEECCCCC
>Mature Secondary Structure 
ANVVVVGSQWGDEGKGKIVDWLSERADVIVRYQGGHNAGHTLVIDGVSYKLSLLPSGLV
CCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCEEEEECCCHHH
RGKLSVIGNGVVVDPHHFVAEVEKLRGQGIDVTPDVLRVAENAPLILSIHRELDAMREGS
HCCCEEECCCEEECCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEHHHHHHHHCCC
NSGLKIGTTKRGIGPAYEDKVGRRAIRVIDLTEPETLRPKVERLLAHHNSLRRGMGLEEI
CCCEEECCCCCCCCCHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHH
AVETILTELTSVADQILPYIDQVWRVLDERRKAGARILFEGAQGALLDNDHGTYPFVTSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEECC
NTVAGQAAAGSGLGPTAIGYVLGITKAYTTRVGEGPFPTELNDEIGEFLGTKGHEFGVVT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEE
GRKRRCGWFDAVIVRQTVRTSGINGIALTKLDVLDGLEEIKICVAYELDGKRIDYLPSSM
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHCCHHHEEEEEEEECCCCEEECCCCCC
GAQARVKPIYETLPGWSETTAGARSWNDLPAQAVKYVRHIEELIGAPVAMLSTSPEREDT
CCCCEECHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCE
ILVTDPFHD
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA