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The map label for this gene is rpoH [H]

Identifier: 23502510

GI number: 23502510

Start: 1600198

End: 1601109

Strand: Reverse

Name: rpoH [H]

Synonym: BR1650

Alternate gene names: 23502510

Gene position: 1601109-1600198 (Counterclockwise)

Preceding gene: 52430462

Following gene: 23502506

Centisome position: 75.96

GC content: 58.33

Gene sequence:

>912_bases
ATGGCCCAGATGAAACTCCCAAGCATTACGTCCGGCGACGGGGGACTGACCCGTTATCTGGAAGAAATTCGCCGTTTTCC
CATGCTTGAGCCGCAGGAAGAATATATGCTGGCCAAGCGTTATCACGAACATGCCGACCCCAAGGCCGCCCACAAGCTGG
TGACGAGCCATCTGCGCCTCGTGGCAAAGATTGCCATGGGCTATCGCGGCTATGGCCTGCCGATCGGCGAAGTGATTTCA
GAAGGCAATGTCGGCCTCATGCAGGCGGTCAAGCGATTCGAGCCGGAACGTGGCTTCCGTCTTGCCACCTATGCCATGTG
GTGGATCAAGGCTTCGATCCAGGAATATATCCTGCGTTCGTGGAGCCTTGTGAAGATGGGCACGACCGCCAACCAGAAGC
GCCTTTTCTTCAATCTGCGCAAGATGAAGAGCAAGATTCAGGCGCTCGACGATGGCGATCTCAACCCGGATCAGGTCAAG
CAGATCGCGACCAAGCTGAATGTCAGCGAGGACGAGGTCGTTTCCATGAACCGCCGCTTGTCCGGCGATGCGTCTCTCAA
TGCGCCGCTGCGCGCTTCGGAAGGCGAATCGGGCGAATGGCAGGACTGGCTCGTCGATGACAGCAACAGCCAGGAACAGG
TTCTGATCGAACAGGACGAGCTGGAAAGCCGCCGCTCGATGCTGGAACAGGCGATGGAAACCCTGAACGACCGTGAGCGC
CGTATTTTCGAGGCTCGTCGTCTCGCCGAAGAGCCAATGACGCTGGAAGATCTTTCCGGTGAATTCGGCATCAGCCGTGA
ACGCGTGCGCCAGATCGAAGTACGCGCCTTTGAGAAGGTTCAGGCCGCCGTCAAGGCCGCGGCCCACAAGCAGCAGAAGG
CGCTGAACCATATCGAGGAAGCGCACGTCTGA

Upstream 100 bases:

>100_bases
AAATGATGCATATATATGTCGGTTCGCGCGGAAGTGCAGGAGGCAGCCGACGCGACAGGTTCGCCAGACGGGAACCTGAA
TTTATAAAGGAGGGTGCTTT

Downstream 100 bases:

>100_bases
TTTCCAATTCCAGGGAATTTTCTCTATGAAAAAGGCCGCACGACGCGGCCTTTTTCAATCAGAGGATCTTCTGCATGAAC
AGAAGATCGAGCCAGCGTCC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MAQMKLPSITSGDGGLTRYLEEIRRFPMLEPQEEYMLAKRYHEHADPKAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVIS
EGNVGLMQAVKRFEPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRKMKSKIQALDDGDLNPDQVK
QIATKLNVSEDEVVSMNRRLSGDASLNAPLRASEGESGEWQDWLVDDSNSQEQVLIEQDELESRRSMLEQAMETLNDRER
RIFEARRLAEEPMTLEDLSGEFGISRERVRQIEVRAFEKVQAAVKAAAHKQQKALNHIEEAHV

Sequences:

>Translated_303_residues
MAQMKLPSITSGDGGLTRYLEEIRRFPMLEPQEEYMLAKRYHEHADPKAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVIS
EGNVGLMQAVKRFEPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRKMKSKIQALDDGDLNPDQVK
QIATKLNVSEDEVVSMNRRLSGDASLNAPLRASEGESGEWQDWLVDDSNSQEQVLIEQDELESRRSMLEQAMETLNDRER
RIFEARRLAEEPMTLEDLSGEFGISRERVRQIEVRAFEKVQAAVKAAAHKQQKALNHIEEAHV
>Mature_302_residues
AQMKLPSITSGDGGLTRYLEEIRRFPMLEPQEEYMLAKRYHEHADPKAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVISE
GNVGLMQAVKRFEPERGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKRLFFNLRKMKSKIQALDDGDLNPDQVKQ
IATKLNVSEDEVVSMNRRLSGDASLNAPLRASEGESGEWQDWLVDDSNSQEQVLIEQDELESRRSMLEQAMETLNDRERR
IFEARRLAEEPMTLEDLSGEFGISRERVRQIEVRAFEKVQAAVKAAAHKQQKALNHIEEAHV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=40.0696864111498, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1789098, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1789448, Length=237, Percent_Identity=27.0042194092827, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34757; Mature: 34626

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMKLPSITSGDGGLTRYLEEIRRFPMLEPQEEYMLAKRYHEHADPKAAHKLVTSHLRL
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VAKIAMGYRGYGLPIGEVISEGNVGLMQAVKRFEPERGFRLATYAMWWIKASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKRLFFNLRKMKSKIQALDDGDLNPDQVKQIATKLNVSEDEVVSMNRRL
CHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHC
SGDASLNAPLRASEGESGEWQDWLVDDSNSQEQVLIEQDELESRRSMLEQAMETLNDRER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
RIFEARRLAEEPMTLEDLSGEFGISRERVRQIEVRAFEKVQAAVKAAAHKQQKALNHIEE
HHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHV
HCC
>Mature Secondary Structure 
AQMKLPSITSGDGGLTRYLEEIRRFPMLEPQEEYMLAKRYHEHADPKAAHKLVTSHLRL
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VAKIAMGYRGYGLPIGEVISEGNVGLMQAVKRFEPERGFRLATYAMWWIKASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKRLFFNLRKMKSKIQALDDGDLNPDQVKQIATKLNVSEDEVVSMNRRL
CHHEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHC
SGDASLNAPLRASEGESGEWQDWLVDDSNSQEQVLIEQDELESRRSMLEQAMETLNDRER
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHH
RIFEARRLAEEPMTLEDLSGEFGISRERVRQIEVRAFEKVQAAVKAAAHKQQKALNHIEE
HHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]