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The map label for this gene is trpE [H]

Identifier: 23502434

GI number: 23502434

Start: 1519315

End: 1521510

Strand: Reverse

Name: trpE [H]

Synonym: BR1570

Alternate gene names: 23502434

Gene position: 1521510-1519315 (Counterclockwise)

Preceding gene: 23502438

Following gene: 23502432

Centisome position: 72.18

GC content: 58.38

Gene sequence:

>2196_bases
ATGAATGCGAAGACTGCGGATAGCGAGATATTCCAGCACGAAACGGCAGGCGGTATCATCGTCGAGCGGGTGCGCCACCT
CACGGCCTATAAGGGCGCCATTGAAAGCTATATCGATGTGCTGAACGAACGGCGCGGTGCGGTGTTCTCGTCCAATTACG
AATATCCGGGCCGCTATACGCGATGGGATACCGCAATTGTCGATCCGCCGGTCGTCATCACGTCGCGTGCCCGCACGATG
CGCATCGAGGCGCTGAACGCGCGCGGCGTCATCCTGCTTCGGCCCATTCTGGATACCGTCAAGGCGCTTTCGGAAGTAAA
GATCGACCAGTCCGGCGAAAACCGTATCGATCTGACGATTGTGGAACCGGTCGGCACCTTCACGGAAGAAGAACGCTCGC
GCATGCCCTCGGTCTTTACGGTGCTGCGCGCCATAGTCGGGCTTTTCTTCTCGGAGGAGGATGCCAATCTCGGCCTTTAT
GGCGCCTTTGGCTATGATCTGGCGTTCCAGTTCGATCCCATCCAGTACAAGCTGAAGCGCCCGGACGACCAGCGTGACCT
CGTGCTGTTCATTCCCGACGAAATCTTCGTCGCCGACCATTATGCGGCGCGCGCCTGGGTGGACCGTTATGAATTTCGCT
GCGGCGGTTCGTCCACGCACGGTCTTGATCGCGCGACGCCGGTGGTGCCTTTCAAGCCATCGGAGCGCAAGCTTGCGCGC
GGCGATCATAATCCGGGTGAATATGCCAGGCTTGTCGAGCGCGCCAAGGAAAGCTTCAAGCGCGGCGACCTGTTCGAGGT
TGTGCCGGGCCAGACCTTCTATGAGCGCTGCCACACGGCGCCGTCGGAGATTTTCCGCCGGCTGAAGTCGATCAATCCTT
CGCCCTATTCCTTTTTCATCAATCTGGGCGAGAGCGAATATCTGGTCGGCGCATCGCCGGAAATGTTTGTGCGCGTCAAT
GGGCGGCGCATCGAGACCTGCCCGATTTCCGGCACCATCAAGCGCGGTGAAGATGCAATTTCGGATTCTGAGCAGATATT
GAAACTGCTTAATTCCAAGAAGGACGAATCCGAGCTGACCATGTGTTCGGATGTGGACCGCAACGACAAGAGCCGCGTTT
GCGAGCCGGGTTCGGTGCGTGTTATCGGTCGCCGCCAGATCGAGATGTATTCCCGCCTGATCCATACGGTCGATCATATC
GAAGGCCGCCTGCGTGACGGCATGGATGCGTTTGACGGCTTCCTCAGCCATGCATGGGCTGTGACGGTGACAGGCGCGCC
GAAGCTGTGGGCAATGCGCTTTCTTGAGGAAAACGAACGCAGCCCGCGCGCATGGTATGGCGGCGCGATCGGCATGATGC
ATTTCAATGGCGATATGAATACAGGGCTGACGCTGCGCACCATCCGCATCAAGGATGGTGTGGCGGAAATCCGTGCAGGG
GCGACGCTTCTGTTCGATTCCAACCCTGACGAGGAAGAAGCCGAGACCGAATTGAAGGCATCGGCCATGATTGCGGCTGT
GCGGGACGCACAGAAGAGCAATCAGATCGCGGAAGAAAGTGTGGCGGCAAAGGTGGGTGAGGGGGTTTCGATCCTGCTGG
TCGATCACGAGGATTCCTTCGTCCATACGCTTGCCAATTATTTCCGCCAGACGGGCGCCAAGGTTTCCACCGTGCGTTCA
CCGGTGGCAGAGGAGATATTCGACCGCGTCAATCCCGATCTGGTGGTGTTATCGCCGGGACCGGGCTCGCCGCAGGATTT
CGATTGCAAGGCGACCATCGATAAGGCGCGCAAGCGCCAGCTTCCGATTTTTGGCGTCTGCCTCGGCCTTCAGGCACTGG
CGGAAGCCTATGGCGGGGCGTTGCGCCAGCTTCGCGTTCCGGTGCATGGCAAGCCTTCACGCATCCGCGTATCAAAGCCG
GAGCGCATTTTCTCCGGCTTGCCGGAGGAAGTGACGGTGGGGCGTTATCATTCGATCTTCGCCGATCCTGAACGCCTGCC
GGATGATTTTCTCGTCACAGCCGAAACGGAAGACGGGATCATCATGGCTTTTGAACATAAACATGAACCGGTGGCAGCCG
TTCAATTCCATCCCGAATCCATCATGATGCTTGGCCATAATGCCGGTATGCGCATGATCGAGAATGTCGTGACGCATCTT
GCAGGCAAGCACAAGGCGCGCCGCACCAACTATTGA

Upstream 100 bases:

>100_bases
GCGGCCTTTTTGTTTGGCTGTTGGAATAAGACCGAAAGCATGTCTCGCGAAGCGGGAACAGGAAATATTCGGAAACGAAA
GAGATTTGGGATAGCCGATC

Downstream 100 bases:

>100_bases
TAGAATATATTCCGCCCTAACTTGAAGCCAGCCGCAGCCAAACCTTGCGGCTGGTTTTTTCATGCTTTGGCGATTCCTGC
CTGTTGCTTGCTCCGGCAAC

Product: anthranilate synthase

Products: NA

Alternate protein names: Glutamine amidotransferase [H]

Number of amino acids: Translated: 731; Mature: 731

Protein sequence:

>731_residues
MNAKTADSEIFQHETAGGIIVERVRHLTAYKGAIESYIDVLNERRGAVFSSNYEYPGRYTRWDTAIVDPPVVITSRARTM
RIEALNARGVILLRPILDTVKALSEVKIDQSGENRIDLTIVEPVGTFTEEERSRMPSVFTVLRAIVGLFFSEEDANLGLY
GAFGYDLAFQFDPIQYKLKRPDDQRDLVLFIPDEIFVADHYAARAWVDRYEFRCGGSSTHGLDRATPVVPFKPSERKLAR
GDHNPGEYARLVERAKESFKRGDLFEVVPGQTFYERCHTAPSEIFRRLKSINPSPYSFFINLGESEYLVGASPEMFVRVN
GRRIETCPISGTIKRGEDAISDSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHTVDHI
EGRLRDGMDAFDGFLSHAWAVTVTGAPKLWAMRFLEENERSPRAWYGGAIGMMHFNGDMNTGLTLRTIRIKDGVAEIRAG
ATLLFDSNPDEEEAETELKASAMIAAVRDAQKSNQIAEESVAAKVGEGVSILLVDHEDSFVHTLANYFRQTGAKVSTVRS
PVAEEIFDRVNPDLVVLSPGPGSPQDFDCKATIDKARKRQLPIFGVCLGLQALAEAYGGALRQLRVPVHGKPSRIRVSKP
ERIFSGLPEEVTVGRYHSIFADPERLPDDFLVTAETEDGIIMAFEHKHEPVAAVQFHPESIMMLGHNAGMRMIENVVTHL
AGKHKARRTNY

Sequences:

>Translated_731_residues
MNAKTADSEIFQHETAGGIIVERVRHLTAYKGAIESYIDVLNERRGAVFSSNYEYPGRYTRWDTAIVDPPVVITSRARTM
RIEALNARGVILLRPILDTVKALSEVKIDQSGENRIDLTIVEPVGTFTEEERSRMPSVFTVLRAIVGLFFSEEDANLGLY
GAFGYDLAFQFDPIQYKLKRPDDQRDLVLFIPDEIFVADHYAARAWVDRYEFRCGGSSTHGLDRATPVVPFKPSERKLAR
GDHNPGEYARLVERAKESFKRGDLFEVVPGQTFYERCHTAPSEIFRRLKSINPSPYSFFINLGESEYLVGASPEMFVRVN
GRRIETCPISGTIKRGEDAISDSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHTVDHI
EGRLRDGMDAFDGFLSHAWAVTVTGAPKLWAMRFLEENERSPRAWYGGAIGMMHFNGDMNTGLTLRTIRIKDGVAEIRAG
ATLLFDSNPDEEEAETELKASAMIAAVRDAQKSNQIAEESVAAKVGEGVSILLVDHEDSFVHTLANYFRQTGAKVSTVRS
PVAEEIFDRVNPDLVVLSPGPGSPQDFDCKATIDKARKRQLPIFGVCLGLQALAEAYGGALRQLRVPVHGKPSRIRVSKP
ERIFSGLPEEVTVGRYHSIFADPERLPDDFLVTAETEDGIIMAFEHKHEPVAAVQFHPESIMMLGHNAGMRMIENVVTHL
AGKHKARRTNY
>Mature_731_residues
MNAKTADSEIFQHETAGGIIVERVRHLTAYKGAIESYIDVLNERRGAVFSSNYEYPGRYTRWDTAIVDPPVVITSRARTM
RIEALNARGVILLRPILDTVKALSEVKIDQSGENRIDLTIVEPVGTFTEEERSRMPSVFTVLRAIVGLFFSEEDANLGLY
GAFGYDLAFQFDPIQYKLKRPDDQRDLVLFIPDEIFVADHYAARAWVDRYEFRCGGSSTHGLDRATPVVPFKPSERKLAR
GDHNPGEYARLVERAKESFKRGDLFEVVPGQTFYERCHTAPSEIFRRLKSINPSPYSFFINLGESEYLVGASPEMFVRVN
GRRIETCPISGTIKRGEDAISDSEQILKLLNSKKDESELTMCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHTVDHI
EGRLRDGMDAFDGFLSHAWAVTVTGAPKLWAMRFLEENERSPRAWYGGAIGMMHFNGDMNTGLTLRTIRIKDGVAEIRAG
ATLLFDSNPDEEEAETELKASAMIAAVRDAQKSNQIAEESVAAKVGEGVSILLVDHEDSFVHTLANYFRQTGAKVSTVRS
PVAEEIFDRVNPDLVVLSPGPGSPQDFDCKATIDKARKRQLPIFGVCLGLQALAEAYGGALRQLRVPVHGKPSRIRVSKP
ERIFSGLPEEVTVGRYHSIFADPERLPDDFLVTAETEDGIIMAFEHKHEPVAAVQFHPESIMMLGHNAGMRMIENVVTHL
AGKHKARRTNY

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=473, Percent_Identity=29.3868921775898, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1788114, Length=413, Percent_Identity=27.6029055690073, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1789760, Length=173, Percent_Identity=40.4624277456647, Blast_Score=124, Evalue=3e-29,
Organism=Escherichia coli, GI1787517, Length=192, Percent_Identity=36.4583333333333, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1786809, Length=226, Percent_Identity=28.3185840707965, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=295, Percent_Identity=34.2372881355932, Blast_Score=163, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6322638, Length=203, Percent_Identity=36.4532019704434, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6324361, Length=142, Percent_Identity=30.9859154929577, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR006805
- InterPro:   IPR006220
- InterPro:   IPR010112
- InterPro:   IPR001317
- InterPro:   IPR015890
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind; PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 81883; Mature: 81883

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKTADSEIFQHETAGGIIVERVRHLTAYKGAIESYIDVLNERRGAVFSSNYEYPGRYT
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
RWDTAIVDPPVVITSRARTMRIEALNARGVILLRPILDTVKALSEVKIDQSGENRIDLTI
CCCCEECCCCEEEECCCEEEEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEE
VEPVGTFTEEERSRMPSVFTVLRAIVGLFFSEEDANLGLYGAFGYDLAFQFDPIQYKLKR
ECCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCEEEECC
PDDQRDLVLFIPDEIFVADHYAARAWVDRYEFRCGGSSTHGLDRATPVVPFKPSERKLAR
CCCCCCEEEEECCCEEEECHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCHHHC
GDHNPGEYARLVERAKESFKRGDLFEVVPGQTFYERCHTAPSEIFRRLKSINPSPYSFFI
CCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEE
NLGESEYLVGASPEMFVRVNGRRIETCPISGTIKRGEDAISDSEQILKLLNSKKDESELT
ECCCCCEEECCCCCEEEEECCEEEEECCCCCCCCCCCHHCCCHHHHHHHHCCCCCCCHHH
MCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHTVDHIEGRLRDGMDAFDGFLSHAWA
EHHCCCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEE
VTVTGAPKLWAMRFLEENERSPRAWYGGAIGMMHFNGDMNTGLTLRTIRIKDGVAEIRAG
EEECCCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHHHHCC
ATLLFDSNPDEEEAETELKASAMIAAVRDAQKSNQIAEESVAAKVGEGVSILLVDHEDSF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHH
VHTLANYFRQTGAKVSTVRSPVAEEIFDRVNPDLVVLSPGPGSPQDFDCKATIDKARKRQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHC
LPIFGVCLGLQALAEAYGGALRQLRVPVHGKPSRIRVSKPERIFSGLPEEVTVGRYHSIF
CCHHHHHHHHHHHHHHHCHHHHEEECCCCCCCCEEEECCCHHHHCCCCCCEECCCHHHHC
ADPERLPDDFLVTAETEDGIIMAFEHKHEPVAAVQFHPESIMMLGHNAGMRMIENVVTHL
CCHHHCCCCEEEEEECCCCEEEEEECCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHH
AGKHKARRTNY
HCCCCHHCCCC
>Mature Secondary Structure
MNAKTADSEIFQHETAGGIIVERVRHLTAYKGAIESYIDVLNERRGAVFSSNYEYPGRYT
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
RWDTAIVDPPVVITSRARTMRIEALNARGVILLRPILDTVKALSEVKIDQSGENRIDLTI
CCCCEECCCCEEEECCCEEEEEEEECCCCEEEEHHHHHHHHHHHHCCCCCCCCCEEEEEE
VEPVGTFTEEERSRMPSVFTVLRAIVGLFFSEEDANLGLYGAFGYDLAFQFDPIQYKLKR
ECCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCEEEECC
PDDQRDLVLFIPDEIFVADHYAARAWVDRYEFRCGGSSTHGLDRATPVVPFKPSERKLAR
CCCCCCEEEEECCCEEEECHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCHHHC
GDHNPGEYARLVERAKESFKRGDLFEVVPGQTFYERCHTAPSEIFRRLKSINPSPYSFFI
CCCCHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEE
NLGESEYLVGASPEMFVRVNGRRIETCPISGTIKRGEDAISDSEQILKLLNSKKDESELT
ECCCCCEEECCCCCEEEEECCEEEEECCCCCCCCCCCHHCCCHHHHHHHHCCCCCCCHHH
MCSDVDRNDKSRVCEPGSVRVIGRRQIEMYSRLIHTVDHIEGRLRDGMDAFDGFLSHAWA
EHHCCCCCCHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCEE
VTVTGAPKLWAMRFLEENERSPRAWYGGAIGMMHFNGDMNTGLTLRTIRIKDGVAEIRAG
EEECCCCHHHHHHHHHHCCCCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCHHHHHCC
ATLLFDSNPDEEEAETELKASAMIAAVRDAQKSNQIAEESVAAKVGEGVSILLVDHEDSF
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHH
VHTLANYFRQTGAKVSTVRSPVAEEIFDRVNPDLVVLSPGPGSPQDFDCKATIDKARKRQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHC
LPIFGVCLGLQALAEAYGGALRQLRVPVHGKPSRIRVSKPERIFSGLPEEVTVGRYHSIF
CCHHHHHHHHHHHHHHHCHHHHEEECCCCCCCCEEEECCCHHHHCCCCCCEECCCHHHHC
ADPERLPDDFLVTAETEDGIIMAFEHKHEPVAAVQFHPESIMMLGHNAGMRMIENVVTHL
CCHHHCCCCEEEEEECCCCEEEEEECCCCCEEEEEECCCEEEEEECCCCHHHHHHHHHHH
AGKHKARRTNY
HCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656657; 11481430 [H]