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The map label for this gene is metF [H]

Identifier: 23502319

GI number: 23502319

Start: 1408788

End: 1409699

Strand: Reverse

Name: metF [H]

Synonym: BR1450

Alternate gene names: 23502319

Gene position: 1409699-1408788 (Counterclockwise)

Preceding gene: 23502320

Following gene: 23502317

Centisome position: 66.88

GC content: 57.57

Gene sequence:

>912_bases
ATGGGTTTTTATGGTCTTTCCCGCCGGCCGGATGTCGGCCAGACCACCCGGGTATCGTTTGAATTCTTCCCTCCCAAGAC
GGAAGAAATGGAACAGCGTCTCTGGGAAACGGTCACGCGGCTTGCGCCGCTCCAGCCTGATTTCGTCTCCGTTACCTATG
GGGCCGGTGGCTCCACGCGAGAGCGCACGGTGCGCACGGTGGCCCGCATTCTCAAGGAAACAGATATCAAACCTGCCGCA
CATCTGACCTGCGTGGATGCAACATGCGAAGATGTGGACCGGGTTGCGCGCGAATTTGCGGCGCTCGGTGTCAATCGTTT
TGTGGCGCTGCGGGGTGATCCGGCCAACGGTATCGGTGAGAAATATGTGCCGACGCCGGGTGGCTATCAGAACGGTGCCG
AGCTGGTCGGTGGACTGCGCAATATTGCGGATTTCGATATTTCCGTTTCGGCCTATCCGGAAAAACACCCGGAAAGCCCC
GATTTTGCAACCGATATCGACATGTTGAAGCGCAAGGTGGACAATGGCGCCACGCGCGCCATCACCCAGTTTTTCTTCGA
GAATGATCTGTATGAGCGTTATGTCGAGCGCGTACGCCGTGCAGGCATCTATATTCCTATCGTTCCGGGCGTGCTGCCGG
TGCATAATTTCAAGCAGGTGCGCAATTTCTGTGCGCGTTCGGCAACCCATATTCCGGCATGGCTTGCCGAACGGTTCGAC
GGGCTGGATAACGATCCGCAAACGCATCAGCTCGTCGCGGCGGCAATTGCCGCCGAGCAGGTGATGGACCTGATCGAGCG
CGGTGTGCAGGATTTCCATTTCTACACGATGAACCGGGCCGATCTGCCTTTTGCCATCTGTCATATGATCGGCATAAGGC
CGAAGACGCAAGCAGCCCTGGAGGTTGCCTGA

Upstream 100 bases:

>100_bases
CATCGCCGATCCGGTTTCGCATGGCTCCCAAAAGTGGCAACCGGTTTTGGGATAAAGACATGCGGCAAAAACAGACAATA
AGCATGACGGAGACAGTTTG

Downstream 100 bases:

>100_bases
AACAAAGAAAGCCCGGCGATTTCGCCGGGCTTTCTCGTTTTCATGCGGGTCGGCCTATCCTCAAAAACCGGAGCCGCCGT
AATGGCTTGATGCATCTCTT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MGFYGLSRRPDVGQTTRVSFEFFPPKTEEMEQRLWETVTRLAPLQPDFVSVTYGAGGSTRERTVRTVARILKETDIKPAA
HLTCVDATCEDVDRVAREFAALGVNRFVALRGDPANGIGEKYVPTPGGYQNGAELVGGLRNIADFDISVSAYPEKHPESP
DFATDIDMLKRKVDNGATRAITQFFFENDLYERYVERVRRAGIYIPIVPGVLPVHNFKQVRNFCARSATHIPAWLAERFD
GLDNDPQTHQLVAAAIAAEQVMDLIERGVQDFHFYTMNRADLPFAICHMIGIRPKTQAALEVA

Sequences:

>Translated_303_residues
MGFYGLSRRPDVGQTTRVSFEFFPPKTEEMEQRLWETVTRLAPLQPDFVSVTYGAGGSTRERTVRTVARILKETDIKPAA
HLTCVDATCEDVDRVAREFAALGVNRFVALRGDPANGIGEKYVPTPGGYQNGAELVGGLRNIADFDISVSAYPEKHPESP
DFATDIDMLKRKVDNGATRAITQFFFENDLYERYVERVRRAGIYIPIVPGVLPVHNFKQVRNFCARSATHIPAWLAERFD
GLDNDPQTHQLVAAAIAAEQVMDLIERGVQDFHFYTMNRADLPFAICHMIGIRPKTQAALEVA
>Mature_302_residues
GFYGLSRRPDVGQTTRVSFEFFPPKTEEMEQRLWETVTRLAPLQPDFVSVTYGAGGSTRERTVRTVARILKETDIKPAAH
LTCVDATCEDVDRVAREFAALGVNRFVALRGDPANGIGEKYVPTPGGYQNGAELVGGLRNIADFDISVSAYPEKHPESPD
FATDIDMLKRKVDNGATRAITQFFFENDLYERYVERVRRAGIYIPIVPGVLPVHNFKQVRNFCARSATHIPAWLAERFDG
LDNDPQTHQLVAAAIAAEQVMDLIERGVQDFHFYTMNRADLPFAICHMIGIRPKTQAALEVA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=281, Percent_Identity=31.6725978647687, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI1790377, Length=303, Percent_Identity=48.1848184818482, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25147436, Length=284, Percent_Identity=26.7605633802817, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI25147441, Length=284, Percent_Identity=26.7605633802817, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321313, Length=283, Percent_Identity=29.3286219081272, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=30.6122448979592, Blast_Score=124, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24662658, Length=266, Percent_Identity=26.6917293233083, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33892; Mature: 33761

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFYGLSRRPDVGQTTRVSFEFFPPKTEEMEQRLWETVTRLAPLQPDFVSVTYGAGGSTR
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHH
ERTVRTVARILKETDIKPAAHLTCVDATCEDVDRVAREFAALGVNRFVALRGDPANGIGE
HHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCC
KYVPTPGGYQNGAELVGGLRNIADFDISVSAYPEKHPESPDFATDIDMLKRKVDNGATRA
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ITQFFFENDLYERYVERVRRAGIYIPIVPGVLPVHNFKQVRNFCARSATHIPAWLAERFD
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHC
GLDNDPQTHQLVAAAIAAEQVMDLIERGVQDFHFYTMNRADLPFAICHMIGIRPKTQAAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCHHH
EVA
CCC
>Mature Secondary Structure 
GFYGLSRRPDVGQTTRVSFEFFPPKTEEMEQRLWETVTRLAPLQPDFVSVTYGAGGSTR
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHH
ERTVRTVARILKETDIKPAAHLTCVDATCEDVDRVAREFAALGVNRFVALRGDPANGIGE
HHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCHHHEEEEECCCCCCCCC
KYVPTPGGYQNGAELVGGLRNIADFDISVSAYPEKHPESPDFATDIDMLKRKVDNGATRA
CCCCCCCCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHH
ITQFFFENDLYERYVERVRRAGIYIPIVPGVLPVHNFKQVRNFCARSATHIPAWLAERFD
HHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHC
GLDNDPQTHQLVAAAIAAEQVMDLIERGVQDFHFYTMNRADLPFAICHMIGIRPKTQAAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCCCHHH
EVA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]