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The map label for this gene is amiD [H]

Identifier: 23502315

GI number: 23502315

Start: 1402831

End: 1403637

Strand: Reverse

Name: amiD [H]

Synonym: BR1444

Alternate gene names: 23502315

Gene position: 1403637-1402831 (Counterclockwise)

Preceding gene: 23502316

Following gene: 23502314

Centisome position: 66.59

GC content: 55.51

Gene sequence:

>807_bases
ATGAGCGGCACATGTGGAACACCGGGTGAAATCTTGCCGGAACTGGTGCTGGAAAAGCCGGACTATAAAGAGGCGGAGCT
TGACCCGTCACCGAATTTCGGGCCGCGCCGCGATGGCAAGCAGCCGGTTTTCCTGATCCTGCATTATACCGGCCTTGCGA
CCGCCAAAGAGGCGATGGATGTGCTGAAATCGCCGGAAATGGAGGTTTCAGCCCATTACGTCGTTCATGAGGATGGTCGT
GTGGTGCAGATGGTTTCGGAAAAGGCGCGCGCATGGCACGCGGGCAAGAGCTTCTGGAAAGGCGAGACCGATATAAATTC
GGCCTCTATCGGCATTGAGATCGTCAATCCTGGCGTTCTGGAAAACTATCCGCCTTTCCATGATGCGCAGATTGAAGCGG
TCATTCGCCTTTGCCAGGATATTTGCAAGCGCCATGAAATCCGGCCGGAGAACGTGCTGGCGCATTCCGATATCGCCCCT
GCGCGCAAGACCGATCCGGGCCAAAGCTTCCCCTGGAAACGCCTGCATGATGCGGGTATCGGCCATTATGTCGAGCCGAC
GCCGATCTGCGGCGGGCGCTTTCTGGCGCGTGGGGAACAGGGGCAGCCTGTGGAGGCGCTCCAGTCCATGCTGGCCCTTT
ACGGCTATGAAATTGCGATTACCGGTATCTTCGATGAGGCTACGGAAATCGTAATTAAGGCCTTTCAGCGGCATTTTCGG
CCACAAAATGTGGACGGTGTAGCCGATGTATCTACTATAGATACCCTCTACAGGCTCAATTTTTCCCTTCAAAATGTTAC
TGCGTGA

Upstream 100 bases:

>100_bases
TGGCGGAAGGCCTGCCGCTCGAATTTATTACCATCGCCAATGCGCGCCTTGCCGTCATAAATGCGGCCTGGGCCAGCATT
GAAAAACAATTGGAGACAGC

Downstream 100 bases:

>100_bases
AGCTGCGGCTGGAGCCTGAGGCTCCAGCGCGCGTATTTTATATTTTATAATAATTTCATAAAAATGGATCAATCTGGCGA
TCGGGTTTTGAGGCTCGGCT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MSGTCGTPGEILPELVLEKPDYKEAELDPSPNFGPRRDGKQPVFLILHYTGLATAKEAMDVLKSPEMEVSAHYVVHEDGR
VVQMVSEKARAWHAGKSFWKGETDINSASIGIEIVNPGVLENYPPFHDAQIEAVIRLCQDICKRHEIRPENVLAHSDIAP
ARKTDPGQSFPWKRLHDAGIGHYVEPTPICGGRFLARGEQGQPVEALQSMLALYGYEIAITGIFDEATEIVIKAFQRHFR
PQNVDGVADVSTIDTLYRLNFSLQNVTA

Sequences:

>Translated_268_residues
MSGTCGTPGEILPELVLEKPDYKEAELDPSPNFGPRRDGKQPVFLILHYTGLATAKEAMDVLKSPEMEVSAHYVVHEDGR
VVQMVSEKARAWHAGKSFWKGETDINSASIGIEIVNPGVLENYPPFHDAQIEAVIRLCQDICKRHEIRPENVLAHSDIAP
ARKTDPGQSFPWKRLHDAGIGHYVEPTPICGGRFLARGEQGQPVEALQSMLALYGYEIAITGIFDEATEIVIKAFQRHFR
PQNVDGVADVSTIDTLYRLNFSLQNVTA
>Mature_267_residues
SGTCGTPGEILPELVLEKPDYKEAELDPSPNFGPRRDGKQPVFLILHYTGLATAKEAMDVLKSPEMEVSAHYVVHEDGRV
VQMVSEKARAWHAGKSFWKGETDINSASIGIEIVNPGVLENYPPFHDAQIEAVIRLCQDICKRHEIRPENVLAHSDIAPA
RKTDPGQSFPWKRLHDAGIGHYVEPTPICGGRFLARGEQGQPVEALQSMLALYGYEIAITGIFDEATEIVIKAFQRHFRP
QNVDGVADVSTIDTLYRLNFSLQNVTA

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=222, Percent_Identity=39.1891891891892, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI1786300, Length=178, Percent_Identity=30.8988764044944, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 29714; Mature: 29583

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTCGTPGEILPELVLEKPDYKEAELDPSPNFGPRRDGKQPVFLILHYTGLATAKEAMD
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHH
VLKSPEMEVSAHYVVHEDGRVVQMVSEKARAWHAGKSFWKGETDINSASIGIEIVNPGVL
HHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEEEECCCCC
ENYPPFHDAQIEAVIRLCQDICKRHEIRPENVLAHSDIAPARKTDPGQSFPWKRLHDAGI
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCCCCHHHHHHCCC
GHYVEPTPICGGRFLARGEQGQPVEALQSMLALYGYEIAITGIFDEATEIVIKAFQRHFR
CCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCC
PQNVDGVADVSTIDTLYRLNFSLQNVTA
CCCCCCCHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
SGTCGTPGEILPELVLEKPDYKEAELDPSPNFGPRRDGKQPVFLILHYTGLATAKEAMD
CCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHH
VLKSPEMEVSAHYVVHEDGRVVQMVSEKARAWHAGKSFWKGETDINSASIGIEIVNPGVL
HHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCHHHCCCCCCCCCCEEEEEEECCCCC
ENYPPFHDAQIEAVIRLCQDICKRHEIRPENVLAHSDIAPARKTDPGQSFPWKRLHDAGI
CCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCCCCCCHHHHHHCCC
GHYVEPTPICGGRFLARGEQGQPVEALQSMLALYGYEIAITGIFDEATEIVIKAFQRHFR
CCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCC
PQNVDGVADVSTIDTLYRLNFSLQNVTA
CCCCCCCHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]