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The map label for this gene is ligA

Identifier: 23502291

GI number: 23502291

Start: 1373732

End: 1375891

Strand: Reverse

Name: ligA

Synonym: BR1420

Alternate gene names: 23502291

Gene position: 1375891-1373732 (Counterclockwise)

Preceding gene: 23502292

Following gene: 23502290

Centisome position: 65.28

GC content: 59.95

Gene sequence:

>2160_bases
ATGTCCGATATTTCCGTCGAGAAACTGACCGAACTCGAAGCTGCCGCCGAACTGGAACGGCTGGCGCGCGCGATTGCTCA
TCACGACGAGCTTTATCACGCCAAGGACAGGCCGGAAATTTCCGATGCGGCTTATGATGCACTGAAGCGGCGCAACGAGG
CGATTGAAGCGCACTTTCCGGCACTTGTCCGCGATGACAGCCCGTCGCGGCGTGTGGGTGCAGCCCCGGCGCTTGCCACC
TTTGCGCCTGTTGTTCACGCGCGGCCCATGCTGTCGCTGGATAATGCCTTTAGTGATGAGGACGTGCGTGATTTTGTTGG
CAGCGTCTATCGCTTTCTGGGCCAACTGCCGGATGATTCCATCGCCTTTACCGCAGAACCGAAGATCGATGGGCTTTCCA
TGTCGATCCGTTACGAGAATGGAATTCTGGTTAGCGGTGCGACACGCGGGGATGGCACAACGGGCGAGAATGTAACAGCC
AATATCCGCACCATTGCTGAAATCCCGAACAGGCTTCCGGCAGGTGCGCCTGCTGTCGTGGAAGTGCGCGGCGAGGTCTA
TATGGCCAAGAGCGACTTTCTGACGCTCAATGCGCAGATGGAGGCGGAGGGCAAGCAGACCTATGTGAACCCGCGCAATA
CGGCAGCAGGTTCGCTGCGCCAGCTTGATGCGAAGGTGACGGCAAGCCGCAAGCTCAGGTTCTTCGCCTATGCCTGGGGG
GAAATGTCGGACATGCCTGCGGATACGCAATTGGGCATGGTCGAGGTTTTTCGCCAATGGGGCTTTCCGGTCAATCCGCT
GATGAAGCGGTTCAACAGCGTGGATGGGTTGCTTGCGCATTATCGCGCCATCGGCATGGAGCGTCCCACGCTCGATTACG
ATATCGACGGTGTTGTTTACAAGGTGGACCGGCTCGACCTGCAAACCCGGCTTGGTTTTCGCTCGCGCAGCCCGCGCTGG
GCGATTGCGCATAAATTCCCGGCGGAACAGGCGCTCACCATTTTGCGCGGCATCGATATTCAGGTGGGCCGCACTGGCGC
GCTGACGCCGGTGGCGCGGCTGGAGCCAATCACCGTCGGCGGTGTGGTTGTGACCAACGCGACGCTGCACAATGAGGACT
ATATCAAGGGCATAGGTCAGAAGGGCGAGCCGATCCGGGAGGGGCGCGATATCCGCATTGGCGATAGCGTGATCGTGCAG
CGCGCGGGCGATGTCATCCCGCAGATCGTGGATGTGGTGCTTGAGGAAGGCAAAAAACGCGGCGAGCCTTATCAGTTTCC
CCATGTCTGCCCCGCCTGCGGCAGCCATGCCGTGCGCGAGGAGGGCGAGGCGGTACGCCGCTGCACCGGCGGGCTGATCT
GCCCTGCGCAGGCGGTGGAACGCATCCGCCATTTTGTGTCGCGCAATGCCTTCGACATTGAGGGGCTTGGCGAAAAGCAG
GTCGAGTTCTTCTTCAATGCGGAAGATCCGGCCCTTTGCATCCGCTCGCCTGCCGATATTTTCACACTGAAAAAGCGGCA
GGAAAACTCGCTGACCAAGCTTCAGAATATCGAAGGCTTTGGCGCAACTTCCGTGAAGAAGCTTTATGACGCCATCGATG
CGCGGCGCGAGATTGCACTCCACCGCTTTCTGTTTGGTCTTGGCATCCGCCATGTGGGCGAGGTGAATGCCAAGCGCCTT
GCCCGCGCCTATCTTTCATATGCCGCTTTTGAGAAGGCGGCGCTGGAGGCTGTGCCGCCAAAAGAAGGCGACCGCACCGA
CAAGGGCAGCGAAGCGTGGCAGGACATGCTGGCCGTGGAGGGGATCGGTTCCATCGTGGCGGAAGCCGTGGTCGATTTTT
ATGGCGAGCCGCATAACCGCGAAGTGCTGGCCGCACTTCTTGCCGAAGTAACGCCGCTTGATGAGGAAGCGCGTGTGGCG
ACCGGTTCGCCCGTCGAGGGCAAGACGGTGGTTTTCACCGGCAGTCTGGAGCGCATGTCACGCGATGAAGCGAAGGCTAT
GGCTGAGCGCCATGGCGCGAAGACGGCAGGCTCTGTCTCCAAGAAGACCGATCTCGTGGTGGCAGGGCCGGGGGCCGGTT
CCAAGCTTGCCAAGGCGACGGAACTGGGCATTGAGGTTATCAATGAAGACGACTGGTTCAAGCTTGTTGGGGAGGATTGA

Upstream 100 bases:

>100_bases
GATAGTCAGGATTTGCTGCTTAGAGCAGTTCCTGTTTTAACGGAATCGCCGGAACCGCTCTGCGCAAAACCGTTTCACAC
TTTTGCTGGAAATGCTCTAG

Downstream 100 bases:

>100_bases
TGGCCGCATTCAATGGCTTCGGGGAGAAGGCCATTCCCTTTCTCAGGGCGCTCGATTTTCACCAGAACCGTGAATGGTTT
CTGGAAAACAGGGATTTGTT

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MSDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFPALVRDDSPSRRVGAAPALAT
FAPVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDSIAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTA
NIRTIAEIPNRLPAGAPAVVEVRGEVYMAKSDFLTLNAQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWG
EMSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVYKVDRLDLQTRLGFRSRSPRW
AIAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQ
RAGDVIPQIVDVVLEEGKKRGEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQ
VEFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIALHRFLFGLGIRHVGEVNAKRL
ARAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVEGIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVA
TGSPVEGKTVVFTGSLERMSRDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED

Sequences:

>Translated_719_residues
MSDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFPALVRDDSPSRRVGAAPALAT
FAPVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDSIAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTA
NIRTIAEIPNRLPAGAPAVVEVRGEVYMAKSDFLTLNAQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWG
EMSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVYKVDRLDLQTRLGFRSRSPRW
AIAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQ
RAGDVIPQIVDVVLEEGKKRGEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQ
VEFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIALHRFLFGLGIRHVGEVNAKRL
ARAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVEGIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVA
TGSPVEGKTVVFTGSLERMSRDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED
>Mature_718_residues
SDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFPALVRDDSPSRRVGAAPALATF
APVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDSIAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTAN
IRTIAEIPNRLPAGAPAVVEVRGEVYMAKSDFLTLNAQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWGE
MSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVYKVDRLDLQTRLGFRSRSPRWA
IAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQR
AGDVIPQIVDVVLEEGKKRGEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQV
EFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIALHRFLFGLGIRHVGEVNAKRLA
RAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVEGIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVAT
GSPVEGKTVVFTGSLERMSRDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=708, Percent_Identity=46.7514124293785, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI87082305, Length=552, Percent_Identity=21.7391304347826, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_BRUA1 (B2S6P4)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001935315.1
- ProteinModelPortal:   B2S6P4
- GeneID:   6327292
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I13460
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 78682; Mature: 78551

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 128-128 BINDING 126-126 BINDING 149-149 BINDING 185-185 BINDING 301-301 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
ALVRDDSPSRRVGAAPALATFAPVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDS
EEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
IAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTANIRTIAEIPNRLPAGAPAVV
EEEEECCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEE
EVRGEVYMAKSDFLTLNAQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWG
EECCCEEEEECCEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
EMSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVY
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
KVDRLDLQTRLGFRSRSPRWAIAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVG
EEEHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCCCCHHHCCCEEEC
GVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQRAGDVIPQIVDVVLEEGKKR
CEEEEEEEECCCHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHC
GEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQ
CCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
VEFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIAL
EEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HRFLFGLGIRHVGEVNAKRLARAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
GIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVATGSPVEGKTVVFTGSLERMS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHH
RDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCEEECCCCEEEECCCC
>Mature Secondary Structure 
SDISVEKLTELEAAAELERLARAIAHHDELYHAKDRPEISDAAYDALKRRNEAIEAHFP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCC
ALVRDDSPSRRVGAAPALATFAPVVHARPMLSLDNAFSDEDVRDFVGSVYRFLGQLPDDS
EEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
IAFTAEPKIDGLSMSIRYENGILVSGATRGDGTTGENVTANIRTIAEIPNRLPAGAPAVV
EEEEECCCCCCEEEEEEECCCEEEECCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEE
EVRGEVYMAKSDFLTLNAQMEAEGKQTYVNPRNTAAGSLRQLDAKVTASRKLRFFAYAWG
EECCCEEEEECCEEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEECC
EMSDMPADTQLGMVEVFRQWGFPVNPLMKRFNSVDGLLAHYRAIGMERPTLDYDIDGVVY
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
KVDRLDLQTRLGFRSRSPRWAIAHKFPAEQALTILRGIDIQVGRTGALTPVARLEPITVG
EEEHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCCCCCHHHCCCEEEC
GVVVTNATLHNEDYIKGIGQKGEPIREGRDIRIGDSVIVQRAGDVIPQIVDVVLEEGKKR
CEEEEEEEECCCHHHHCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHC
GEPYQFPHVCPACGSHAVREEGEAVRRCTGGLICPAQAVERIRHFVSRNAFDIEGLGEKQ
CCCCCCCCCCCHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCE
VEFFFNAEDPALCIRSPADIFTLKKRQENSLTKLQNIEGFGATSVKKLYDAIDARREIAL
EEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
HRFLFGLGIRHVGEVNAKRLARAYLSYAAFEKAALEAVPPKEGDRTDKGSEAWQDMLAVE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
GIGSIVAEAVVDFYGEPHNREVLAALLAEVTPLDEEARVATGSPVEGKTVVFTGSLERMS
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHHHHH
RDEAKAMAERHGAKTAGSVSKKTDLVVAGPGAGSKLAKATELGIEVINEDDWFKLVGED
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHCCEEECCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA