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The map label for this gene is tcaB [H]

Identifier: 23502272

GI number: 23502272

Start: 1354772

End: 1355983

Strand: Reverse

Name: tcaB [H]

Synonym: BR1401

Alternate gene names: 23502272

Gene position: 1355983-1354772 (Counterclockwise)

Preceding gene: 23502273

Following gene: 23502271

Centisome position: 64.33

GC content: 60.4

Gene sequence:

>1212_bases
ATGGCTTCCAGTCTTGTGCGCAATGCCATTGTTCTTGGCCTTCTTTCGGCCATCGGTCCTTTTGCGATTGATATGTATCT
ACCGGCCCTGCCGACCATTGCCACCGATCTGCGGGCGGAAACCGGCGCGGTGCAGATGAGCCTGCTCGCCTTCTTTCTGG
CACTGGCGCTGGCGCAGCTTTTCTGCGGGCCCCTGTCCGATATGGTCGGGCGTAAACTGCCGCTTTATGGCGGGCTGGTG
CTCTTCGCCATAGGCAGCATCGGTTCGGCGCTTGCGGCCAATATCGACGTGCTGGTGCTGTTCCGTTTCGTCCAGGGATT
GGGGGCTGCGGCAGGCATGGTGATACCGCGCGCCATCGTGCGCGATCTTCATATGGGTGTCGAGGCCGCGCGCCTTATGT
CGCTTTTGATGCTGGTCTTTTCGATTTCGCCGATTCTGGCCCCGCTGACTGGCTCCATCATCATAAGCTTTTTCGGCTGG
CGCGGGGTTTTCTGGGCCGTGTTGCTTGCCGCCGGCATCGGCATCATACTGGTTGCGACCGCGCAGAAGGAAACGCGCAG
CGTCGAGGCGCGGCTTGAGAGCAATATCAGCATCGCATTGCGCGGTTACGGGCGGCTGTTGAAGGACCGTCATTTCCTCG
GCCTCGTCTTCGTGGGCGGTTTCGGTCTTGCGTCCTTGTTCGTTTATCTGGCGAATTCCGCCTTCGTGCTGATCGACCAT
TATGGCCTGACACCGATACAATATGCCGTGGCATTTTCCGTCAATGCCGTGTCGTTCTTCGGCGTTTCGCAGCTCAACGG
CTGGCTTGGCGCGCGTTTCGGCCTTGGCCGGGTGATGCGCTTTGCTGCTTGCGGCTTTGCCGCAGCAATGCTTGCCATGT
TCGCGGCGGCGCTGATGGGGCATCAATCACTCTGGCTGGTCGCAAGCTTCCTCTTTGTCGGTTACGGCTTCCTTGGGCTG
GTTATTCCAGCTACGGCGGTGCTGGCGCTCGAAGAACACGGCGAGATTGCCGGAACGGCATCTGCATTGATGGGCACGCT
TCACTTCGTCATTGGTGGCGTGGCGATTGGTATTGTCGGAGCCTATTCCGATGGCACGGCGCTGCCGATGATTGGCGGCA
TCACCGGCTGTGCGCTGGCGGCGTTCGTGCTGGCGCAGATCGTCCTTGCCCGCAAGTCGAGAAAGCGCGGCAGAGCAACG
GCGGCAGCCTGA

Upstream 100 bases:

>100_bases
GCCGGTTTTTCCAGCCTGATGAAACCATCCTCCCAGGTGACATGGGCGAGCCGAAATCGGCGCGGGCACCTCACGAAACA
TTGATGAAAGCGCGTCTCCC

Downstream 100 bases:

>100_bases
TTTTCAGGGGAAATGAAGTGGAGGCTTCTGTTGCCAGGTGCCTCCGAACCCCGCCTTAAGGGGCTAACCCTAAGGACTTT
AGAGTGGGTTTCCCGCACCG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MASSLVRNAIVLGLLSAIGPFAIDMYLPALPTIATDLRAETGAVQMSLLAFFLALALAQLFCGPLSDMVGRKLPLYGGLV
LFAIGSIGSALAANIDVLVLFRFVQGLGAAAGMVIPRAIVRDLHMGVEAARLMSLLMLVFSISPILAPLTGSIIISFFGW
RGVFWAVLLAAGIGIILVATAQKETRSVEARLESNISIALRGYGRLLKDRHFLGLVFVGGFGLASLFVYLANSAFVLIDH
YGLTPIQYAVAFSVNAVSFFGVSQLNGWLGARFGLGRVMRFAACGFAAAMLAMFAAALMGHQSLWLVASFLFVGYGFLGL
VIPATAVLALEEHGEIAGTASALMGTLHFVIGGVAIGIVGAYSDGTALPMIGGITGCALAAFVLAQIVLARKSRKRGRAT
AAA

Sequences:

>Translated_403_residues
MASSLVRNAIVLGLLSAIGPFAIDMYLPALPTIATDLRAETGAVQMSLLAFFLALALAQLFCGPLSDMVGRKLPLYGGLV
LFAIGSIGSALAANIDVLVLFRFVQGLGAAAGMVIPRAIVRDLHMGVEAARLMSLLMLVFSISPILAPLTGSIIISFFGW
RGVFWAVLLAAGIGIILVATAQKETRSVEARLESNISIALRGYGRLLKDRHFLGLVFVGGFGLASLFVYLANSAFVLIDH
YGLTPIQYAVAFSVNAVSFFGVSQLNGWLGARFGLGRVMRFAACGFAAAMLAMFAAALMGHQSLWLVASFLFVGYGFLGL
VIPATAVLALEEHGEIAGTASALMGTLHFVIGGVAIGIVGAYSDGTALPMIGGITGCALAAFVLAQIVLARKSRKRGRAT
AAA
>Mature_402_residues
ASSLVRNAIVLGLLSAIGPFAIDMYLPALPTIATDLRAETGAVQMSLLAFFLALALAQLFCGPLSDMVGRKLPLYGGLVL
FAIGSIGSALAANIDVLVLFRFVQGLGAAAGMVIPRAIVRDLHMGVEAARLMSLLMLVFSISPILAPLTGSIIISFFGWR
GVFWAVLLAAGIGIILVATAQKETRSVEARLESNISIALRGYGRLLKDRHFLGLVFVGGFGLASLFVYLANSAFVLIDHY
GLTPIQYAVAFSVNAVSFFGVSQLNGWLGARFGLGRVMRFAACGFAAAMLAMFAAALMGHQSLWLVASFLFVGYGFLGLV
IPATAVLALEEHGEIAGTASALMGTLHFVIGGVAIGIVGAYSDGTALPMIGGITGCALAAFVLAQIVLARKSRKRGRATA
AA

Specific function: Involved In Sulfonamide (Sulfathiazole) And Bicyclomycin Resistance. Probable Membrane Translocase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=401, Percent_Identity=33.1670822942643, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI48994889, Length=339, Percent_Identity=31.858407079646, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1790146, Length=238, Percent_Identity=31.5126050420168, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87082312, Length=347, Percent_Identity=28.8184438040346, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787065, Length=342, Percent_Identity=26.6081871345029, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6319517, Length=202, Percent_Identity=24.7524752475248, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42003; Mature: 41872

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSLVRNAIVLGLLSAIGPFAIDMYLPALPTIATDLRAETGAVQMSLLAFFLALALAQL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCGPLSDMVGRKLPLYGGLVLFAIGSIGSALAANIDVLVLFRFVQGLGAAAGMVIPRAIV
HCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
RDLHMGVEAARLMSLLMLVFSISPILAPLTGSIIISFFGWRGVFWAVLLAAGIGIILVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
AQKETRSVEARLESNISIALRGYGRLLKDRHFLGLVFVGGFGLASLFVYLANSAFVLIDH
CCHHHHHHHHHHHCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
YGLTPIQYAVAFSVNAVSFFGVSQLNGWLGARFGLGRVMRFAACGFAAAMLAMFAAALMG
CCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HQSLWLVASFLFVGYGFLGLVIPATAVLALEEHGEIAGTASALMGTLHFVIGGVAIGIVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEE
AYSDGTALPMIGGITGCALAAFVLAQIVLARKSRKRGRATAAA
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ASSLVRNAIVLGLLSAIGPFAIDMYLPALPTIATDLRAETGAVQMSLLAFFLALALAQL
CHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FCGPLSDMVGRKLPLYGGLVLFAIGSIGSALAANIDVLVLFRFVQGLGAAAGMVIPRAIV
HCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
RDLHMGVEAARLMSLLMLVFSISPILAPLTGSIIISFFGWRGVFWAVLLAAGIGIILVAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
AQKETRSVEARLESNISIALRGYGRLLKDRHFLGLVFVGGFGLASLFVYLANSAFVLIDH
CCHHHHHHHHHHHCCCEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
YGLTPIQYAVAFSVNAVSFFGVSQLNGWLGARFGLGRVMRFAACGFAAAMLAMFAAALMG
CCCCHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HQSLWLVASFLFVGYGFLGLVIPATAVLALEEHGEIAGTASALMGTLHFVIGGVAIGIVG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEE
AYSDGTALPMIGGITGCALAAFVLAQIVLARKSRKRGRATAAA
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 9202461 [H]