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The map label for this gene is thyA

Identifier: 23502270

GI number: 23502270

Start: 1352993

End: 1353787

Strand: Reverse

Name: thyA

Synonym: BR1399

Alternate gene names: 23502270

Gene position: 1353787-1352993 (Counterclockwise)

Preceding gene: 23502271

Following gene: 23502269

Centisome position: 64.23

GC content: 55.47

Gene sequence:

>795_bases
ATGCGCACCTATCTTGATCTTCTCCAGCATGTGCTCGACCACGGCGTTGACCGTGACGACCGCACGGGAACGGGCACACG
CTCGGTCTTCGGCTATCAGATGCGGTTCGACCTGGAGGAGGGGTTTCCGGTTCTCACGACCAAGAAACTCCATTTGCGTT
CGATCATTCATGAACTCTTGTGGTTCCTGAAAGGCGATACGAACATTGCCTATCTGAAAGAAAACGGCGTTACGATCTGG
GATGAATGGGCCGATGAAAACGGCGATCTGGGGCCGGTTTATGGCTATCAGTGGCGTTCATGGCCTGCGCCGGACGGACG
GCATATCGACCAGATCGCGAATCTTTTGAAGATGTTGCACACAAATCCTCAGTCGCGGCGTCTGATTGTTTCGGCGTGGA
ACCCGGCGCTGGTGGATGAAATGGCTCTGCCGCCCTGCCACTGCCTGTTCCAGTTCTATGTCGCCAATGGCAGGCTTTCC
TGCCAGCTTTACCAGCGTTCCGCGGATATTTTCCTCGGCGTGCCGTTCAATATCGCCTCCTATGCTCTGTTGACGATGAT
GATCGCGCAGGTGACGGGGCTGAAACCGGGCGAGTTCATCCATACGCTGGGCGATGCCCATATTTATTCCAACCATTTCG
AGCAGGCACGACTACAACTGACCCGCACGCCGAAGAAGCTGCCGGTGATGCATATCAACCCGGACGTGAAGGATCTCTTT
GCCTTTCGTTTCGAGGATTTCCGGCTGGATGGCTATGAAGCCGACCCCACGATCAAAGCGCCTATCGCGGTATAG

Upstream 100 bases:

>100_bases
ACGCCGTGGCACATTTGCCTGTTGAATCCGAAGTCCCGGTGCCTCACACCGGGGTTTTTCCTTATAGTGATCGGGAAACG
AATCGGCCCGGAACAAATCT

Downstream 100 bases:

>100_bases
TCATGAAGAAACCACTCGTTTCCATCGTCGTGGCGGCCTCCGAAAACAATGTGATCGGGCAGGAAAACGATATGCCATGG
CGTCTTTCCACCGACCTGAA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MRTYLDLLQHVLDHGVDRDDRTGTGTRSVFGYQMRFDLEEGFPVLTTKKLHLRSIIHELLWFLKGDTNIAYLKENGVTIW
DEWADENGDLGPVYGYQWRSWPAPDGRHIDQIANLLKMLHTNPQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLS
CQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLF
AFRFEDFRLDGYEADPTIKAPIAV

Sequences:

>Translated_264_residues
MRTYLDLLQHVLDHGVDRDDRTGTGTRSVFGYQMRFDLEEGFPVLTTKKLHLRSIIHELLWFLKGDTNIAYLKENGVTIW
DEWADENGDLGPVYGYQWRSWPAPDGRHIDQIANLLKMLHTNPQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLS
CQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLF
AFRFEDFRLDGYEADPTIKAPIAV
>Mature_264_residues
MRTYLDLLQHVLDHGVDRDDRTGTGTRSVFGYQMRFDLEEGFPVLTTKKLHLRSIIHELLWFLKGDTNIAYLKENGVTIW
DEWADENGDLGPVYGYQWRSWPAPDGRHIDQIANLLKMLHTNPQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLS
CQLYQRSADIFLGVPFNIASYALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLF
AFRFEDFRLDGYEADPTIKAPIAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=52.6690391459075, Blast_Score=296, Evalue=1e-80,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=65.1515151515152, Blast_Score=380, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=47.1631205673759, Blast_Score=270, Evalue=8e-73,
Organism=Saccharomyces cerevisiae, GI6324648, Length=290, Percent_Identity=42.4137931034483, Blast_Score=213, Evalue=4e-56,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=52.2968197879859, Blast_Score=292, Evalue=2e-79,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_BRUA2 (Q2YS22)

Other databases:

- EMBL:   AM040264
- RefSeq:   YP_414790.1
- HSSP:   P0A884
- ProteinModelPortal:   Q2YS22
- SMR:   Q2YS22
- STRING:   Q2YS22
- GeneID:   3788985
- GenomeReviews:   AM040264_GR
- KEGG:   bmf:BAB1_1418
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- ProtClustDB:   PRK01827
- BioCyc:   BMEL359391:BAB1_1418-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30330; Mature: 30330

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTYLDLLQHVLDHGVDRDDRTGTGTRSVFGYQMRFDLEEGFPVLTTKKLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLKGDTNIAYLKENGVTIWDEWADENGDLGPVYGYQWRSWPAPDGRHIDQIANLLKMLH
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TNPQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLF
HHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
AFRFEDFRLDGYEADPTIKAPIAV
HHHHHHEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRTYLDLLQHVLDHGVDRDDRTGTGTRSVFGYQMRFDLEEGFPVLTTKKLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLKGDTNIAYLKENGVTIWDEWADENGDLGPVYGYQWRSWPAPDGRHIDQIANLLKMLH
HHHCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
TNPQSRRLIVSAWNPALVDEMALPPCHCLFQFYVANGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCEEEEEECCCHHHHHHCCCHHHHHHHHHHCCCEEEEEEEECCCCEEEECCHHHHH
YALLTMMIAQVTGLKPGEFIHTLGDAHIYSNHFEQARLQLTRTPKKLPVMHINPDVKDLF
HHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHH
AFRFEDFRLDGYEADPTIKAPIAV
HHHHHHEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA