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The map label for this gene is folA [H]

Identifier: 23502269

GI number: 23502269

Start: 1352472

End: 1352990

Strand: Reverse

Name: folA [H]

Synonym: BR1398

Alternate gene names: 23502269

Gene position: 1352990-1352472 (Counterclockwise)

Preceding gene: 23502270

Following gene: 23502268

Centisome position: 64.19

GC content: 57.8

Gene sequence:

>519_bases
ATGAAGAAACCACTCGTTTCCATCGTCGTGGCGGCCTCCGAAAACAATGTGATCGGGCAGGAAAACGATATGCCATGGCG
TCTTTCCACCGACCTGAAGCGGTTCAAGGCGCTGACTTTGGGCAAGCCGGTCATCATGGGGCGGCGCACCTGGCAGTCGC
TTGGCCGTCCCCTGCCGGGGCGGGCCAATATCGTCATTACCCGTGATGCCGATTTCCGGGCAGAAGGTGCCGAGGTGGTC
GGTTCGCTGGAGGATGCGCTAGCGCTTGCGCGCAAGTTTGCGGCGGAAGGCGGGGTGGACGAAATCTGTGTGATCGGCGG
GGGACAAATCTATGTACAGGCCATGCCGCTTGCCGATCGCTTACACCTGACGCGCGTTCTCTCTGTCATCGAAGGCGATA
CATATTTTCCTGAAATCAATCCGCAGGACTGGCAATTGCTTTCGAGCGAGGATGTGCCCGCCGGTGACAAAGACAGCTAT
CCAACACGCTATATGCTGTACGAAAGGCGCGTTTCGTAA

Upstream 100 bases:

>100_bases
ACCCGGACGTGAAGGATCTCTTTGCCTTTCGTTTCGAGGATTTCCGGCTGGATGGCTATGAAGCCGACCCCACGATCAAA
GCGCCTATCGCGGTATAGTC

Downstream 100 bases:

>100_bases
AGCATAGGACAGGAATTTTGCCGCCTTTGCGTTGAAAGCGGCCCTCGCGATGCCTATAAAACGGAAACATTAGTTGATGA
CGGAAGGCCAAGCTTGCTTT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MKKPLVSIVVAASENNVIGQENDMPWRLSTDLKRFKALTLGKPVIMGRRTWQSLGRPLPGRANIVITRDADFRAEGAEVV
GSLEDALALARKFAAEGGVDEICVIGGGQIYVQAMPLADRLHLTRVLSVIEGDTYFPEINPQDWQLLSSEDVPAGDKDSY
PTRYMLYERRVS

Sequences:

>Translated_172_residues
MKKPLVSIVVAASENNVIGQENDMPWRLSTDLKRFKALTLGKPVIMGRRTWQSLGRPLPGRANIVITRDADFRAEGAEVV
GSLEDALALARKFAAEGGVDEICVIGGGQIYVQAMPLADRLHLTRVLSVIEGDTYFPEINPQDWQLLSSEDVPAGDKDSY
PTRYMLYERRVS
>Mature_172_residues
MKKPLVSIVVAASENNVIGQENDMPWRLSTDLKRFKALTLGKPVIMGRRTWQSLGRPLPGRANIVITRDADFRAEGAEVV
GSLEDALALARKFAAEGGVDEICVIGGGQIYVQAMPLADRLHLTRVLSVIEGDTYFPEINPQDWQLLSSEDVPAGDKDSY
PTRYMLYERRVS

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=156, Percent_Identity=36.5384615384615, Blast_Score=95, Evalue=3e-20,
Organism=Homo sapiens, GI28827793, Length=157, Percent_Identity=36.3057324840764, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1786233, Length=168, Percent_Identity=44.6428571428571, Blast_Score=139, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17505899, Length=176, Percent_Identity=27.8409090909091, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324810, Length=188, Percent_Identity=30.8510638297872, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116008026, Length=164, Percent_Identity=31.0975609756098, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24647458, Length=164, Percent_Identity=31.0975609756098, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19059; Mature: 19059

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPLVSIVVAASENNVIGQENDMPWRLSTDLKRFKALTLGKPVIMGRRTWQSLGRPLPG
CCCCEEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCC
RANIVITRDADFRAEGAEVVGSLEDALALARKFAAEGGVDEICVIGGGQIYVQAMPLADR
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEECCHHH
LHLTRVLSVIEGDTYFPEINPQDWQLLSSEDVPAGDKDSYPTRYMLYERRVS
HHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MKKPLVSIVVAASENNVIGQENDMPWRLSTDLKRFKALTLGKPVIMGRRTWQSLGRPLPG
CCCCEEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHCCCCEEECCHHHHHHCCCCCC
RANIVITRDADFRAEGAEVVGSLEDALALARKFAAEGGVDEICVIGGGQIYVQAMPLADR
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEECCHHH
LHLTRVLSVIEGDTYFPEINPQDWQLLSSEDVPAGDKDSYPTRYMLYERRVS
HHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]