Click here to switch to the map view.

The map label for this gene is lysN [H]

Identifier: 23502249

GI number: 23502249

Start: 1333819

End: 1335051

Strand: Reverse

Name: lysN [H]

Synonym: BR1378

Alternate gene names: 23502249

Gene position: 1335051-1333819 (Counterclockwise)

Preceding gene: 23502250

Following gene: 23502247

Centisome position: 63.34

GC content: 57.34

Gene sequence:

>1233_bases
GTGTTGGACTGGGAAAATGTATTTGCAACGCGCTCGAAACGTATGCGCGCATCGGAAATCCGTGAACTGCTGAAGCTTCT
GGAACGGCCAGATATCATTTCCTTCGCAGGCGGCATTCCTGATCCCGCTCTTTTCCCGCATGATGAATTCCAGGCAGCCT
ACAAGGATATTTTGGGTGGCCCGGAAGCCAATGCTGCGCTGCAATATTCCATTTCGGAAGGCTACAAGCCGCTGCGCACA
TGGCTGGTTGGCGAACTCGCCAAGATCGGCATTCCCTGCACCGAAGATAATGTGTTCATCACGTCTGGCTCGCAGCAGGC
GCTCGATTATCTGGGCAAGCTGTTTCTGTCGCCGGGCGATACTGCGCTCGTAACTGCGCCGACATATCTTGGCGCGCTGC
AAGCCTTCAACGCCTATGAACCGAACTATGACACGCTGGCGCTTAAGGGCAATCGCACGCCGCAATCCTATGCGCAGACT
GCGCAGAAGGCTGGCGGACAGGTCAAGTTCGCTTATCTTTCGGCTGATTTCAGCAATCCGACTGGCGAGACGGTCGATCG
TGCGGGTCGCGAAAAGCTGCTGGCACATGCCGACGAACTGGATATCCCAGTGATCGAAGATGCCGCCTATCAGTATCTTC
GCTATAATGGCGATGCGATCCCGCCGATCCTGGCGCTCGACATTGCCCGCAATGGCGGCGATATCGAGAAGACCCGCACC
ATCTATTGCGGCTCGTTCTCCAAGACGCTGGCACCGGGCTTGCGCGTCGGCTATGTGGTTGCCTCCAAGACCGTCATCCG
CAAGCTCGTCCTGATGAAGCAGGCGGCGGACCTTCATTCTTCCACCATCAACCAGATTGCGATCCAGCACGTTGCGGTGC
ATGGCTTCGATGCACAGGTGGCAAAGCTGCACGGCGTTTACAAACATCGCCGTGACAGGATGCTGGAGGCTCTGGCGAAA
TATATGCCGGAAGGCGTTCACTGGACAAAGCCGGAAGGCGGGATGTTCGTCTGGGTCACACTGCCGGAAGGCATGGACGG
CGCTTCGCTGCTGGCCGTTTCCATCGACAGTGAAAAGGTGGCATTCGTTCCGGGCAAGGCGTTCTTTGCCGACGGAACCG
GTGCGAATACGCTGCGCCTTAGCTATTCCTGCGCCAATGACGAGATGATCGATGAAGGCATCAAGCGGCTTGGCCGCCTG
ATCCGCGCGCATACGAAATCCGCTGCGGCGTAA

Upstream 100 bases:

>100_bases
GAATATCTTTATTCCATAGAAACATGGGAAAAGCTGTTCTCGTCCGCTGAAAATTGCGCTAAACCACCATCGTCAATAGC
TGGGTTTGAGGAGACTTCCC

Downstream 100 bases:

>100_bases
GATTTTGTATCAAAGGTAAAAAAACCGGGCCTTGCGCCCGGTTTTTTATTGGAATGGAGAATTGCAGCTTTGCCGTGGTC
CGGCGAAGGGCTGGTAAGTA

Product: class I aminotransferase

Products: NA

Alternate protein names: 2-aminoadipate aminotransferase; Alpha-aminoadipate aminotransferase; AAA-AT; AadAT [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MLDWENVFATRSKRMRASEIRELLKLLERPDIISFAGGIPDPALFPHDEFQAAYKDILGGPEANAALQYSISEGYKPLRT
WLVGELAKIGIPCTEDNVFITSGSQQALDYLGKLFLSPGDTALVTAPTYLGALQAFNAYEPNYDTLALKGNRTPQSYAQT
AQKAGGQVKFAYLSADFSNPTGETVDRAGREKLLAHADELDIPVIEDAAYQYLRYNGDAIPPILALDIARNGGDIEKTRT
IYCGSFSKTLAPGLRVGYVVASKTVIRKLVLMKQAADLHSSTINQIAIQHVAVHGFDAQVAKLHGVYKHRRDRMLEALAK
YMPEGVHWTKPEGGMFVWVTLPEGMDGASLLAVSIDSEKVAFVPGKAFFADGTGANTLRLSYSCANDEMIDEGIKRLGRL
IRAHTKSAAA

Sequences:

>Translated_410_residues
MLDWENVFATRSKRMRASEIRELLKLLERPDIISFAGGIPDPALFPHDEFQAAYKDILGGPEANAALQYSISEGYKPLRT
WLVGELAKIGIPCTEDNVFITSGSQQALDYLGKLFLSPGDTALVTAPTYLGALQAFNAYEPNYDTLALKGNRTPQSYAQT
AQKAGGQVKFAYLSADFSNPTGETVDRAGREKLLAHADELDIPVIEDAAYQYLRYNGDAIPPILALDIARNGGDIEKTRT
IYCGSFSKTLAPGLRVGYVVASKTVIRKLVLMKQAADLHSSTINQIAIQHVAVHGFDAQVAKLHGVYKHRRDRMLEALAK
YMPEGVHWTKPEGGMFVWVTLPEGMDGASLLAVSIDSEKVAFVPGKAFFADGTGANTLRLSYSCANDEMIDEGIKRLGRL
IRAHTKSAAA
>Mature_410_residues
MLDWENVFATRSKRMRASEIRELLKLLERPDIISFAGGIPDPALFPHDEFQAAYKDILGGPEANAALQYSISEGYKPLRT
WLVGELAKIGIPCTEDNVFITSGSQQALDYLGKLFLSPGDTALVTAPTYLGALQAFNAYEPNYDTLALKGNRTPQSYAQT
AQKAGGQVKFAYLSADFSNPTGETVDRAGREKLLAHADELDIPVIEDAAYQYLRYNGDAIPPILALDIARNGGDIEKTRT
IYCGSFSKTLAPGLRVGYVVASKTVIRKLVLMKQAADLHSSTINQIAIQHVAVHGFDAQVAKLHGVYKHRRDRMLEALAK
YMPEGVHWTKPEGGMFVWVTLPEGMDGASLLAVSIDSEKVAFVPGKAFFADGTGANTLRLSYSCANDEMIDEGIKRLGRL
IRAHTKSAAA

Specific function: Catalyzes the transfer of an amino group between 2- oxoadipate (2-OA) and glutamate (Glu) to yield alpha-aminodipate (AAA). It can also transaminate glutamate, leucine, and aromatic amino acids. It also conbtributes in the biosynthesis of other amino acid

COG id: COG1167

COG function: function code KE; Transcriptional regulators containing a DNA-binding HTH domain and an aminotransferase domain (MocR family) and their eukaryotic orthologs

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7705897, Length=419, Percent_Identity=30.5489260143198, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI33469970, Length=419, Percent_Identity=30.5489260143198, Blast_Score=176, Evalue=3e-44,
Organism=Escherichia coli, GI1787710, Length=384, Percent_Identity=26.8229166666667, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1790797, Length=338, Percent_Identity=25.4437869822485, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI48994949, Length=408, Percent_Identity=26.4705882352941, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788722, Length=383, Percent_Identity=27.4151436031332, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6321000, Length=272, Percent_Identity=28.6764705882353, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21356535, Length=418, Percent_Identity=23.2057416267943, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.6.1.39 [H]

Molecular weight: Translated: 44744; Mature: 44744

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDWENVFATRSKRMRASEIRELLKLLERPDIISFAGGIPDPALFPHDEFQAAYKDILGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCC
PEANAALQYSISEGYKPLRTWLVGELAKIGIPCTEDNVFITSGSQQALDYLGKLFLSPGD
CCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCC
TALVTAPTYLGALQAFNAYEPNYDTLALKGNRTPQSYAQTAQKAGGQVKFAYLSADFSNP
CEEEECHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEEECCCCC
TGETVDRAGREKLLAHADELDIPVIEDAAYQYLRYNGDAIPPILALDIARNGGDIEKTRT
CCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEE
IYCGSFSKTLAPGLRVGYVVASKTVIRKLVLMKQAADLHSSTINQIAIQHVAVHGFDAQV
EEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHH
AKLHGVYKHRRDRMLEALAKYMPEGVHWTKPEGGMFVWVTLPEGMDGASLLAVSIDSEKV
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCEEEEEEECCCEE
AFVPGKAFFADGTGANTLRLSYSCANDEMIDEGIKRLGRLIRAHTKSAAA
EEECCCEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDWENVFATRSKRMRASEIRELLKLLERPDIISFAGGIPDPALFPHDEFQAAYKDILGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCC
PEANAALQYSISEGYKPLRTWLVGELAKIGIPCTEDNVFITSGSQQALDYLGKLFLSPGD
CCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCC
TALVTAPTYLGALQAFNAYEPNYDTLALKGNRTPQSYAQTAQKAGGQVKFAYLSADFSNP
CEEEECHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEEEEEECCCCC
TGETVDRAGREKLLAHADELDIPVIEDAAYQYLRYNGDAIPPILALDIARNGGDIEKTRT
CCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCEE
IYCGSFSKTLAPGLRVGYVVASKTVIRKLVLMKQAADLHSSTINQIAIQHVAVHGFDAQV
EEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHH
AKLHGVYKHRRDRMLEALAKYMPEGVHWTKPEGGMFVWVTLPEGMDGASLLAVSIDSEKV
HHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCCCCEEEEEEECCCEE
AFVPGKAFFADGTGANTLRLSYSCANDEMIDEGIKRLGRLIRAHTKSAAA
EEECCCEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA