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The map label for this gene is cobE [H]

Identifier: 23502177

GI number: 23502177

Start: 1261430

End: 1261825

Strand: Reverse

Name: cobE [H]

Synonym: BR1301

Alternate gene names: 23502177

Gene position: 1261825-1261430 (Counterclockwise)

Preceding gene: 23502178

Following gene: 23502176

Centisome position: 59.86

GC content: 58.33

Gene sequence:

>396_bases
GTGGCTGATCCGAAATTCTATCTTGGAATGGGGTGCTCGTCGGCTGTCACTTATGATGAGCTTCTGACCCTTGCCGAAGA
GGCCCTGACAAAAGGCGGGTGTTCGCGACCTGATGGTATCGCAACACTGAGCACCAAACGTGGCAATCCTGCATGGGTGC
AGCTTGCGGTCCATTATGGCTGCGAATTGCGGTTCTTCGACGCTTCACGGCTTGAAGATGAAACGCCGCGGCTAAACAGC
CCCTCTCAGGAAACTTTTAAAGCCGTTGGGTGCCATGGCGTGGCGGAGGCGGCTGCGCTGGCTGCCGCAGGCCCGAACGG
CCATCTGGTGGTTGGGAAAATCGCATCGGCCCGGTCCACCGCCGCCCTGGCGATGGTTCCAACGTTTCCGTTATAA

Upstream 100 bases:

>100_bases
CACCGTCTGGATCGTTCGGAAACCACGATGATCCAGCGTATTGCCAACACGCTGGGCATCAAGCCGGAAGAACTGCATAA
GGTGCGGCAGGCGTCGTCTT

Downstream 100 bases:

>100_bases
TCGGAACCGCTCTATCAAGGCGGGGTCTGTCAGCATCCGCGCTTCAGGAAGGGAAAATCGTGACTGTTCATTTCATTGGC
GCCGGTCCGGGCGCAGCCGA

Product: precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MADPKFYLGMGCSSAVTYDELLTLAEEALTKGGCSRPDGIATLSTKRGNPAWVQLAVHYGCELRFFDASRLEDETPRLNS
PSQETFKAVGCHGVAEAAALAAAGPNGHLVVGKIASARSTAALAMVPTFPL

Sequences:

>Translated_131_residues
MADPKFYLGMGCSSAVTYDELLTLAEEALTKGGCSRPDGIATLSTKRGNPAWVQLAVHYGCELRFFDASRLEDETPRLNS
PSQETFKAVGCHGVAEAAALAAAGPNGHLVVGKIASARSTAALAMVPTFPL
>Mature_130_residues
ADPKFYLGMGCSSAVTYDELLTLAEEALTKGGCSRPDGIATLSTKRGNPAWVQLAVHYGCELRFFDASRLEDETPRLNSP
SQETFKAVGCHGVAEAAALAAAGPNGHLVVGKIASARSTAALAMVPTFPL

Specific function: Unknown

COG id: COG2073

COG function: function code H; Cobalamin biosynthesis protein CbiG

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002750 [H]

Pfam domain/function: PF01890 CbiG [H]

EC number: 2.1.1.131

Molecular weight: Translated: 13684; Mature: 13552

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPKFYLGMGCSSAVTYDELLTLAEEALTKGGCSRPDGIATLSTKRGNPAWVQLAVHYG
CCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEECC
CELRFFDASRLEDETPRLNSPSQETFKAVGCHGVAEAAALAAAGPNGHLVVGKIASARST
CEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCC
AALAMVPTFPL
EEEEECCCCCC
>Mature Secondary Structure 
ADPKFYLGMGCSSAVTYDELLTLAEEALTKGGCSRPDGIATLSTKRGNPAWVQLAVHYG
CCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEEEEEECC
CELRFFDASRLEDETPRLNSPSQETFKAVGCHGVAEAAALAAAGPNGHLVVGKIASARST
CEEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEECCCCCCC
AALAMVPTFPL
EEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211520 [H]