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The map label for this gene is tpiA

Identifier: 23502016

GI number: 23502016

Start: 1115475

End: 1116239

Strand: Reverse

Name: tpiA

Synonym: BR1138

Alternate gene names: 23502016

Gene position: 1116239-1115475 (Counterclockwise)

Preceding gene: 23502023

Following gene: 23502015

Centisome position: 52.96

GC content: 59.48

Gene sequence:

>765_bases
ATGACTCCGGGAATCCGTCCGCTGGTTGCTGGCAACTGGAAAATGAATGGCAAGGGAGAATCCCTGACCGAACTGCGCGC
CATCGCTGCGGGCCTCAGCTCCGACCTCGGCCGCAAGCTCGATGCGGTCATATGTGTGCCGGCCACCTTGCTTTCGCGTG
CGGCTGAAACGCTGGAAGGCGAAACGGTCGGCCTTGGCGGACAGGATGCCCATTTCAAGACATCCGGCGCGCATACGGGC
GACATTTCGCCGGAAATGCTCAAGGAAGCTGGTGCCACCCATGTCATTCTTGGCCATTCCGAGCGCCGCACCGATCATCA
CGAGAGCAATAAGCTCATTTGCGCCAAGACGGAAGCCGCATGGGCTGCGGGGCTGGTGGCTATCGTCTGCGTTGGAGAAA
CCGCCAGCGAGCGAAAGGCGGAGCGTGCGCTCGATGTCATTGGCGACCAGCTTTCCGGTTCGCTGCCGGATGGGGTTACG
GCGGAAAACACAATCATTGCCTATGAACCCGTATGGGCTATCGGCACCGGGTTGACGCCGACGGTTCAGGATGTTCGTGC
AGCGCACGCCTTCATGCGTGAACAGTTGATCGAACGTTTCGGCGCAAAAGGCGCGCATCTGCGCCTTCTTTATGGGGGTT
CGGTGAAGCCGTCCAATGCTGCCGAATTGCTCGGTGTTGCAGATGTTGACGGCGCTCTTGTCGGCGGCGCGAGTTTGAAG
GCGGCAGACTTCCTCGCCATATGCGAAACCTATCGCAATCTATAA

Upstream 100 bases:

>100_bases
CATTGCGGCGCGAATACGCTTAGTCAGTGGCAAATTTTGGCGCGGCCCTGCTTCGTGAAATGGGCTGGTGTCTGCCGTTT
GAAAAGGCGAGGAGAACAAC

Downstream 100 bases:

>100_bases
AGCCGGTATTACTTCACGCTATCTTGGGCGTGGGGCTTGGATTATCGGACAATAGCGTGTAAAGAGCCGCTCAAGTTCAG
GATATAAAGACCGTTTCATA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 1; Triose-phosphate isomerase 1

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTPGIRPLVAGNWKMNGKGESLTELRAIAAGLSSDLGRKLDAVICVPATLLSRAAETLEGETVGLGGQDAHFKTSGAHTG
DISPEMLKEAGATHVILGHSERRTDHHESNKLICAKTEAAWAAGLVAIVCVGETASERKAERALDVIGDQLSGSLPDGVT
AENTIIAYEPVWAIGTGLTPTVQDVRAAHAFMREQLIERFGAKGAHLRLLYGGSVKPSNAAELLGVADVDGALVGGASLK
AADFLAICETYRNL

Sequences:

>Translated_254_residues
MTPGIRPLVAGNWKMNGKGESLTELRAIAAGLSSDLGRKLDAVICVPATLLSRAAETLEGETVGLGGQDAHFKTSGAHTG
DISPEMLKEAGATHVILGHSERRTDHHESNKLICAKTEAAWAAGLVAIVCVGETASERKAERALDVIGDQLSGSLPDGVT
AENTIIAYEPVWAIGTGLTPTVQDVRAAHAFMREQLIERFGAKGAHLRLLYGGSVKPSNAAELLGVADVDGALVGGASLK
AADFLAICETYRNL
>Mature_253_residues
TPGIRPLVAGNWKMNGKGESLTELRAIAAGLSSDLGRKLDAVICVPATLLSRAAETLEGETVGLGGQDAHFKTSGAHTGD
ISPEMLKEAGATHVILGHSERRTDHHESNKLICAKTEAAWAAGLVAIVCVGETASERKAERALDVIGDQLSGSLPDGVTA
ENTIIAYEPVWAIGTGLTPTVQDVRAAHAFMREQLIERFGAKGAHLRLLYGGSVKPSNAAELLGVADVDGALVGGASLKA
ADFLAICETYRNL

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=250, Percent_Identity=40.8, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI226529917, Length=250, Percent_Identity=40.8, Blast_Score=156, Evalue=2e-38,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=41.8326693227092, Blast_Score=174, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI17536593, Length=245, Percent_Identity=44.0816326530612, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=44.1295546558704, Blast_Score=194, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28572004, Length=241, Percent_Identity=47.3029045643153, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI28572008, Length=234, Percent_Identity=47.8632478632479, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28572006, Length=234, Percent_Identity=47.8632478632479, Blast_Score=182, Evalue=2e-46,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS1_BRUAB (Q57D01)

Other databases:

- EMBL:   AE017223
- RefSeq:   YP_221844.1
- ProteinModelPortal:   Q57D01
- SMR:   Q57D01
- GeneID:   3339730
- GenomeReviews:   AE017223_GR
- KEGG:   bmb:BruAb1_1144
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q57D01
- ProtClustDB:   PRK00042
- BioCyc:   BABO262698:BRUAB1_1144-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26485; Mature: 26354

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 99-99 ACT_SITE 169-169 BINDING 12-12 BINDING 14-14

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGIRPLVAGNWKMNGKGESLTELRAIAAGLSSDLGRKLDAVICVPATLLSRAAETLEG
CCCCCCCEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCC
ETVGLGGQDAHFKTSGAHTGDISPEMLKEAGATHVILGHSERRTDHHESNKLICAKTEAA
CEECCCCCCCCEECCCCCCCCCCHHHHHHCCCCEEEECCCHHCCCCCCCCCEEEEECHHH
WAAGLVAIVCVGETASERKAERALDVIGDQLSGSLPDGVTAENTIIAYEPVWAIGTGLTP
HHHHEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHCCCCCH
TVQDVRAAHAFMREQLIERFGAKGAHLRLLYGGSVKPSNAAELLGVADVDGALVGGASLK
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHEEEECCCCEEECCCCHH
AADFLAICETYRNL
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TPGIRPLVAGNWKMNGKGESLTELRAIAAGLSSDLGRKLDAVICVPATLLSRAAETLEG
CCCCCCEEECCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHCC
ETVGLGGQDAHFKTSGAHTGDISPEMLKEAGATHVILGHSERRTDHHESNKLICAKTEAA
CEECCCCCCCCEECCCCCCCCCCHHHHHHCCCCEEEECCCHHCCCCCCCCCEEEEECHHH
WAAGLVAIVCVGETASERKAERALDVIGDQLSGSLPDGVTAENTIIAYEPVWAIGTGLTP
HHHHEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHCCCCCH
TVQDVRAAHAFMREQLIERFGAKGAHLRLLYGGSVKPSNAAELLGVADVDGALVGGASLK
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHEEEECCCCEEECCCCHH
AADFLAICETYRNL
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA