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The map label for this gene is epsN [H]

Identifier: 23501416

GI number: 23501416

Start: 519796

End: 520899

Strand: Reverse

Name: epsN [H]

Synonym: BR0521

Alternate gene names: 23501416

Gene position: 520899-519796 (Counterclockwise)

Preceding gene: 23501417

Following gene: 23501415

Centisome position: 24.71

GC content: 47.19

Gene sequence:

>1104_bases
ATGGATATACCAGTTTACTCTCCCTATCTCTGTGGGAATTTCAAAAAGTATGTGAACGAATGCCTTGATACAGGGTGGAT
CTCGTCGAGGGGTGAATTCATATCTCGCTTTGAAGATGCATTTGCACAATATGTCGATGTTCCGTCTGCGACTTCAGTTG
CAAACGGAACGGTGGCACTACATCTCGCTCTAGATGCTCTGGGCATCGGGGCGGGCGATGAAGTCATTGTACCGACCTTT
ACCTATATCGCTTCAGTTAACACTATCTTGCAAACCGGGGCAACACCGGTTTATGTGGACTCTCTCGAAAATACATTGCA
GATAGATCCAGAGGGGGTGCGACTGGCGATTACAGAGCGCACTAAGGCTGTAATGGTTGTTCATCTCTATGGGCATCCAT
GTGACATGGATTCGATCCGAGAGATTTGTGACGAAAAATCGCTACTGCTCGTCGAAGACTGTGCTGAAGGATTCGGAACT
AAATGGAAAAACAGTCACGTCGGCACGTTTGGCGACGTGGCGACGTTTAGTTTCTTTGGGAACAAGACAATTACGACCGG
TGAAGGCGGGATGGTGCTAGCGCGCAATCCTCAAGTCATGGAAAAATGCCGACATCTCAAAAGTCAAGGTACTTCGCCTA
CACGAGAATACTGGCATGATGCGCTTGCGTATAATTACAGAATGACAAATATTCAAGCAGCAATCGGCCTGTCGCAAATT
GAAATGGCAGATGAAATACTCTCCCTTAAAGCCAGGACAGCTGCCTCTTATGCCAGCAAGTTAGCTGGATTGCCGCTTCG
TATGCACACCCCTGTGGGAGACGTTAAACATTCATATTGGATGTGCTCTATTGTACTTGATAACTCGGAACACAGAGAGC
CGCTGCGCCAACATTTAAGGGAGAATGGTGTAGATACACGACCGTTTTTCCCGCCAGCCCATCGTATGCCTCACAGCGCT
TCCACAGGATCTTACCCTGTTGCTGATAGCTTATCCGCTCGTGGGTTGAACCTGCCAAGCCTCCCACACATTACTGATGT
AGAAATCAGTTTTGTTTGTGATTTGGTCAGGAGTTATTTTTCTAATCATTCCAACCACATTTAG

Upstream 100 bases:

>100_bases
GTATCTGAGATGATAGCAGAAGACTTAGCGGCGGCGGCTCGTGAGAATTTGCTGCGCGAACATGGTCACCAATTTTTCGC
CCCTAAGGAATGATCCATAC

Downstream 100 bases:

>100_bases
TGAGACGATTTCGTATGATATCGTATATGGCTAATGTCTGGAAGGTACGCCACTTCTGGTGGCACCTTTCAATGTCTGAT
TTACGTGGGCGCTTCAGGCG

Product: perosamine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDIPVYSPYLCGNFKKYVNECLDTGWISSRGEFISRFEDAFAQYVDVPSATSVANGTVALHLALDALGIGAGDEVIVPTF
TYIASVNTILQTGATPVYVDSLENTLQIDPEGVRLAITERTKAVMVVHLYGHPCDMDSIREICDEKSLLLVEDCAEGFGT
KWKNSHVGTFGDVATFSFFGNKTITTGEGGMVLARNPQVMEKCRHLKSQGTSPTREYWHDALAYNYRMTNIQAAIGLSQI
EMADEILSLKARTAASYASKLAGLPLRMHTPVGDVKHSYWMCSIVLDNSEHREPLRQHLRENGVDTRPFFPPAHRMPHSA
STGSYPVADSLSARGLNLPSLPHITDVEISFVCDLVRSYFSNHSNHI

Sequences:

>Translated_367_residues
MDIPVYSPYLCGNFKKYVNECLDTGWISSRGEFISRFEDAFAQYVDVPSATSVANGTVALHLALDALGIGAGDEVIVPTF
TYIASVNTILQTGATPVYVDSLENTLQIDPEGVRLAITERTKAVMVVHLYGHPCDMDSIREICDEKSLLLVEDCAEGFGT
KWKNSHVGTFGDVATFSFFGNKTITTGEGGMVLARNPQVMEKCRHLKSQGTSPTREYWHDALAYNYRMTNIQAAIGLSQI
EMADEILSLKARTAASYASKLAGLPLRMHTPVGDVKHSYWMCSIVLDNSEHREPLRQHLRENGVDTRPFFPPAHRMPHSA
STGSYPVADSLSARGLNLPSLPHITDVEISFVCDLVRSYFSNHSNHI
>Mature_367_residues
MDIPVYSPYLCGNFKKYVNECLDTGWISSRGEFISRFEDAFAQYVDVPSATSVANGTVALHLALDALGIGAGDEVIVPTF
TYIASVNTILQTGATPVYVDSLENTLQIDPEGVRLAITERTKAVMVVHLYGHPCDMDSIREICDEKSLLLVEDCAEGFGT
KWKNSHVGTFGDVATFSFFGNKTITTGEGGMVLARNPQVMEKCRHLKSQGTSPTREYWHDALAYNYRMTNIQAAIGLSQI
EMADEILSLKARTAASYASKLAGLPLRMHTPVGDVKHSYWMCSIVLDNSEHREPLRQHLRENGVDTRPFFPPAHRMPHSA
STGSYPVADSLSARGLNLPSLPHITDVEISFVCDLVRSYFSNHSNHI

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI145693159, Length=367, Percent_Identity=33.5149863760218, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI2367285, Length=377, Percent_Identity=28.3819628647215, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 40494; Mature: 40494

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIPVYSPYLCGNFKKYVNECLDTGWISSRGEFISRFEDAFAQYVDVPSATSVANGTVAL
CCCCCCCCHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
HLALDALGIGAGDEVIVPTFTYIASVNTILQTGATPVYVDSLENTLQIDPEGVRLAITER
EEEEHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCEEEEEEEC
TKAVMVVHLYGHPCDMDSIREICDEKSLLLVEDCAEGFGTKWKNSHVGTFGDVATFSFFG
CCEEEEEEEECCCCCHHHHHHHHCCCCEEEHHHHHHHCCCCCCCCCCCCCCCHHEEEEEC
NKTITTGEGGMVLARNPQVMEKCRHLKSQGTSPTREYWHDALAYNYRMTNIQAAIGLSQI
CCEEEECCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEHHHHHHCHHHH
EMADEILSLKARTAASYASKLAGLPLRMHTPVGDVKHSYWMCSIVLDNSEHREPLRQHLR
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHH
ENGVDTRPFFPPAHRMPHSASTGSYPVADSLSARGLNLPSLPHITDVEISFVCDLVRSYF
HCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SNHSNHI
HCCCCCC
>Mature Secondary Structure
MDIPVYSPYLCGNFKKYVNECLDTGWISSRGEFISRFEDAFAQYVDVPSATSVANGTVAL
CCCCCCCCHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
HLALDALGIGAGDEVIVPTFTYIASVNTILQTGATPVYVDSLENTLQIDPEGVRLAITER
EEEEHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCEEEEEEEC
TKAVMVVHLYGHPCDMDSIREICDEKSLLLVEDCAEGFGTKWKNSHVGTFGDVATFSFFG
CCEEEEEEEECCCCCHHHHHHHHCCCCEEEHHHHHHHCCCCCCCCCCCCCCCHHEEEEEC
NKTITTGEGGMVLARNPQVMEKCRHLKSQGTSPTREYWHDALAYNYRMTNIQAAIGLSQI
CCEEEECCCCEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEHHHHHHCHHHH
EMADEILSLKARTAASYASKLAGLPLRMHTPVGDVKHSYWMCSIVLDNSEHREPLRQHLR
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEEECCCHHHHHHHHHHH
ENGVDTRPFFPPAHRMPHSASTGSYPVADSLSARGLNLPSLPHITDVEISFVCDLVRSYF
HCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SNHSNHI
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]