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The map label for this gene is yieH [H]

Identifier: 23501390

GI number: 23501390

Start: 487465

End: 488151

Strand: Reverse

Name: yieH [H]

Synonym: BR0489

Alternate gene names: 23501390

Gene position: 488151-487465 (Counterclockwise)

Preceding gene: 23501391

Following gene: 23501386

Centisome position: 23.16

GC content: 59.83

Gene sequence:

>687_bases
ATGGCGGCACCGTCCCTCATTATTTTCGATTGCGATGGTGTTCTGGTCGATTCCGAGATCATCGCCGCAGAAGTCGAATC
CGCCCTCCTGACCGAATCCGGCTATCCGATTGCCGCCGATGAAATGGCCGAACGTTTCGCAGGCCTCACCTGGCAGGATA
TCCTGCTGACGGTGGAGCGCGAGGCGGGCATTCCGCTTTCCGCGTCGCTCCTCGACAAGTCGGAGCGCATTCTGGATGAA
AAGCTGAAGAACGAGGTCAACGCGGTGGAAGACATCGTGGAGGTGGTTTCCGCACTCAAGCTGCCGAAATGCATCTGCTC
GAACTCCACGAGCCTGCGGCTTGAAAACATGCTGAAGCGCGTCGGCCTTTACGAGCTCTTCGCACCCAATATTTTCTCCG
CCAAGGAAGTGGGCAGCGGAAAGACCAAGCCTGCCCCGGACGTGTTTCTCCACGCGGCGCGCCATTTCGGCGTCGATCCG
GCCAATGTGCTGGTGATCGAGGATTCCGTCCATGGAGTTCATGGCGCGCGCGCAGCCGGTATGCGCGTCATCGGTTTTAC
GGGCGGGGCGCACACCTATCCCGGCCATGCCGACAAGCTGACCGATGCGGGAGCCGAAACGGTTATCCACCGCCACAAGG
ATTTGCAGAGCGTGATCGACGCCCTGTCCATCTGGACAGATGCCTGA

Upstream 100 bases:

>100_bases
GTCGTTCCGGCGATTGCCGGTCGTCTTTTCCCTTTCACGCATTGCCAGACGGGAGGCCCTTCCCGCTGGATCAAAATGCT
TCAACAAGAAGGTTACGCTT

Downstream 100 bases:

>100_bases
GCACAGTCCGGAAAGAAAATTCTGACGCCTTGAATTGTCGCTCAGGCGGCAGAGCATTTGTTGCGGCCTTTTGGCGGCGC
ATTGAGAATGGCGAGCAGTT

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MAAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVEREAGIPLSASLLDKSERILDE
KLKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKRVGLYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDP
ANVLVIEDSVHGVHGARAAGMRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA

Sequences:

>Translated_228_residues
MAAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVEREAGIPLSASLLDKSERILDE
KLKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKRVGLYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDP
ANVLVIEDSVHGVHGARAAGMRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA
>Mature_227_residues
AAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVEREAGIPLSASLLDKSERILDEK
LKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKRVGLYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDPA
NVLVIEDSVHGVHGARAAGMRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates phosphoenolpyruvate and AMP [H]

COG id: COG0637

COG function: function code R; Predicted phosphatase/phosphohexomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1790151, Length=189, Percent_Identity=29.1005291005291, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1789046, Length=181, Percent_Identity=29.2817679558011, Blast_Score=60, Evalue=1e-10,
Organism=Drosophila melanogaster, GI116008157, Length=187, Percent_Identity=33.6898395721925, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI17137324, Length=189, Percent_Identity=32.2751322751323, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129151, Length=187, Percent_Identity=27.807486631016, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 24454; Mature: 24323

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVER
CCCCCEEEEECCCEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHEEEEC
EAGIPLSASLLDKSERILDEKLKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHH
VGLYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDPANVLVIEDSVHGVHGARAAG
HCHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCC
MRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAPSLIIFDCDGVLVDSEIIAAEVESALLTESGYPIAADEMAERFAGLTWQDILLTVER
CCCCEEEEECCCEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHEEEEC
EAGIPLSASLLDKSERILDEKLKNEVNAVEDIVEVVSALKLPKCICSNSTSLRLENMLKR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHH
VGLYELFAPNIFSAKEVGSGKTKPAPDVFLHAARHFGVDPANVLVIEDSVHGVHGARAAG
HCHHHHHCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCC
MRVIGFTGGAHTYPGHADKLTDAGAETVIHRHKDLQSVIDALSIWTDA
EEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]