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The map label for this gene is ispE

Identifier: 23501298

GI number: 23501298

Start: 398711

End: 399610

Strand: Reverse

Name: ispE

Synonym: BR0394

Alternate gene names: 23501298

Gene position: 399610-398711 (Counterclockwise)

Preceding gene: 23501299

Following gene: 23501297

Centisome position: 18.96

GC content: 60.22

Gene sequence:

>900_bases
ATGACAGCATTCCAGAACGCCGGACCATCGATAACGCGCCTTGCGCCTGCAAAGATCAATCTGGCGCTGCATGTGACCGG
GCGACGTGACGACGGCTATCATCTTCTCGACATGCTGGTGGTCTTTGCCGATCACGGCGATCGCATCCACATCGAAAAAG
CCGGCAGCGACAGTTTTACGGTCAGCGGCCCTTTTGCAAGCGGCATCCCCGCCGGGCGGGGCAATCTGGTGCTGAAAGCG
CGCGATGCGCTGCGCCAGCATGGCGGGCCAGACCTTTCACCTGTTGCCATTCATCTGGAAAAGAACCTGCCTATTGCTTC
CGGCATTGGCGGCGGATCGAGCGATGCGGCAGCAACCCTGCTTGCGCTCAACACGCTCTGGCAACTCGATCTCGATTTTG
AAATGCTGGCCGCTATCGGCCTTTCCCTCGGTGCAGACCTTCCCATGTGCCTGCATGGCGCAGCCCATGGCACACCGCTG
ATCGCGCGCGGCATTGGTGAAGAGCTGAACGATGTTTCCGGAATTGCGGCCCTTCCCATGCTGCTCGTCAATGACGGTAC
CGCGCTTGCCACGCCCGACGTGTTCCGCGCGCTCACCCGCCGCGAGAACGCCCCCCTGCCGCCTCCGGCCTGTGAAGGGA
CAGACGCCCTTTGCGCCTATCTGCGTGAAACACGCAATGATCTTCTGCCCGCAGCCATCAGCCTTGCACCGCAGATCGAA
CCCAAACTCGCCCTGCTTCGCGCCAAGGGTGCACTTTATGCGCAAATGTCCGGCTCCGGCGCAACATGCTTTGCCATTTT
TAGCGATGAATCAGCCCTGACACGCGCGGCAAAAGAGATTGCGGATGAAAATCCGGGCTGGTTTGCAGTTCCTTCGCACA
GCTTTCCTTCCAGAGCCTGA

Upstream 100 bases:

>100_bases
CACAGAACCGTGCCCATGGCACGCTGGTGCAAGATCCCAAGACCGGCGAATATTACGTCCGCAAGGATTGAGCCCCCGGC
TGTAGTGACGAATTACCCCG

Downstream 100 bases:

>100_bases
TCTTTCTCCCATCGTCTTCGTTTTAAGTACGGATCGTTACGCCGCGCCAGTTTTTGCGCGAATTTTCGCGCGAAATGGTA
TTGCAAAATAGCCCTATTGT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTAFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADHGDRIHIEKAGSDSFTVSGPFASGIPAGRGNLVLKA
RDALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATLLALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPL
IARGIGEELNDVSGIAALPMLLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIE
PKLALLRAKGALYAQMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA

Sequences:

>Translated_299_residues
MTAFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADHGDRIHIEKAGSDSFTVSGPFASGIPAGRGNLVLKA
RDALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATLLALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPL
IARGIGEELNDVSGIAALPMLLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIE
PKLALLRAKGALYAQMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA
>Mature_298_residues
TAFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADHGDRIHIEKAGSDSFTVSGPFASGIPAGRGNLVLKAR
DALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATLLALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPLI
ARGIGEELNDVSGIAALPMLLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIEP
KLALLRAKGALYAQMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=283, Percent_Identity=32.5088339222615, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_BRUA2 (Q2YMB5)

Other databases:

- EMBL:   AM040264
- RefSeq:   YP_413883.1
- ProteinModelPortal:   Q2YMB5
- SMR:   Q2YMB5
- STRING:   Q2YMB5
- GeneID:   3788767
- GenomeReviews:   AM040264_GR
- KEGG:   bmf:BAB1_0423
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   RIYNGLR
- ProtClustDB:   PRK14608
- BioCyc:   BMEL359391:BAB1_0423-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31189; Mature: 31058

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: ACT_SITE 18-18 ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADHGDRIHIEKAGSDSFT
CCCCCCCCCCEEEECCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEE
VSGPFASGIPAGRGNLVLKARDALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATL
EECCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCCHHHHHH
LALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPLIARGIGEELNDVSGIAALPM
HHHHHHHEECCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHCE
LLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIE
EEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PKLALLRAKGALYAQMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA
HHHEEEEECCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCC
>Mature Secondary Structure 
TAFQNAGPSITRLAPAKINLALHVTGRRDDGYHLLDMLVVFADHGDRIHIEKAGSDSFT
CCCCCCCCCEEEECCEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEE
VSGPFASGIPAGRGNLVLKARDALRQHGGPDLSPVAIHLEKNLPIASGIGGGSSDAAATL
EECCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCCHHHHHH
LALNTLWQLDLDFEMLAAIGLSLGADLPMCLHGAAHGTPLIARGIGEELNDVSGIAALPM
HHHHHHHEECCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHCCHHHHHHHHHHHHCE
LLVNDGTALATPDVFRALTRRENAPLPPPACEGTDALCAYLRETRNDLLPAAISLAPQIE
EEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PKLALLRAKGALYAQMSGSGATCFAIFSDESALTRAAKEIADENPGWFAVPSHSFPSRA
HHHEEEEECCEEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA