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The map label for this gene is ywbI [H]

Identifier: 23501231

GI number: 23501231

Start: 335686

End: 336576

Strand: Reverse

Name: ywbI [H]

Synonym: BR0324

Alternate gene names: 23501231

Gene position: 336576-335686 (Counterclockwise)

Preceding gene: 23501238

Following gene: 23501230

Centisome position: 15.97

GC content: 59.82

Gene sequence:

>891_bases
ATGAATTTGAGAAGCTTTCAGGCCTTTGCGGAAGTGGTGCGACAAGGCGGTTTTTCCGCAGCAGCGCGCACCATCAATGC
GACCCAATCCACCGTCAGCAAGGCGGTTAAGCAGCTTGAGGAAGAGCTGCAACTGGTTCTTCTCGACCGGGCCGTATCCC
CGCCGCGCCTCACTGCGGCTGGCGAAATCGTCTATCGCCGCGCACGAGCAATGCTGACGGAAAAAGATGACATGCTGGCC
GAACTGGGCGAATTGCGTGGTTTGAAGCGCGGCCTGTTGCGTCTGGGCCTGCCGCCGATCGGATCCAGTGTTCTGTTCGC
GCCAGTTTTTGCCGCCTATACGAGCCTTTATCCCGATATCGACATCCAGCTTGTCGAACATGGCAGCAAGCGGCTGGAGG
AGCTTCTTCTGGCGGGCGATGTGGAACTGGCGGCTTCGCTCCTGCCGGTGGAGGAGAGCTTCGCATGGCAGGATGTGCGC
TGCGAACCGGTGGTGGCGCTGCTGCCCGCAAGCCACCCCGTCTCGGACGATGACGGCGTGACACTGCGCGAGATCGCCAA
CCTACCCTTCATTCTTTTTGAAAGCGGCTTTGCGCTCAATCACATTATTTTGAGCGCCTGCCAGCGGCATGGGTTTTCAC
CCAATGTCGCCGTACGATCCAGCCAGATCGATTTCATCGTGGAGCTTGTCGCAGCCGGCATGGGGATCGGTTTTCTGCCG
CAGATGATCGCCAAACAGCGCCACCATCCGGGTGTGTGCATCCACCGGGTCAAGGATGCGGATGTGAACTGGCACATGGC
TTTCGTCTGGCGCAAGGGCGCGTTTCTCTCCCATGCCGCACGCGCCTGGCTTGCGCTGTGCGAAGGCGCATCCACCCCTG
CAAAAGCTTGA

Upstream 100 bases:

>100_bases
TCAGGCAGATAATGCCTTTGCTCATGCTGAATATAACCTCCGGCGCTCTATTCTATAAATGAATTGATTTCATTACGGCT
ATTCTGATATGGAATGACTT

Downstream 100 bases:

>100_bases
AAACCGGGCATAAATGCCTACCTTTTCTGGCAGGCTCCATGATTGGAGCCGCTTCAGCTAAAGGCTATCCATTTATGCGA
GGAGGCTATGATGGAAGGTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNLRSFQAFAEVVRQGGFSAAARTINATQSTVSKAVKQLEEELQLVLLDRAVSPPRLTAAGEIVYRRARAMLTEKDDMLA
ELGELRGLKRGLLRLGLPPIGSSVLFAPVFAAYTSLYPDIDIQLVEHGSKRLEELLLAGDVELAASLLPVEESFAWQDVR
CEPVVALLPASHPVSDDDGVTLREIANLPFILFESGFALNHIILSACQRHGFSPNVAVRSSQIDFIVELVAAGMGIGFLP
QMIAKQRHHPGVCIHRVKDADVNWHMAFVWRKGAFLSHAARAWLALCEGASTPAKA

Sequences:

>Translated_296_residues
MNLRSFQAFAEVVRQGGFSAAARTINATQSTVSKAVKQLEEELQLVLLDRAVSPPRLTAAGEIVYRRARAMLTEKDDMLA
ELGELRGLKRGLLRLGLPPIGSSVLFAPVFAAYTSLYPDIDIQLVEHGSKRLEELLLAGDVELAASLLPVEESFAWQDVR
CEPVVALLPASHPVSDDDGVTLREIANLPFILFESGFALNHIILSACQRHGFSPNVAVRSSQIDFIVELVAAGMGIGFLP
QMIAKQRHHPGVCIHRVKDADVNWHMAFVWRKGAFLSHAARAWLALCEGASTPAKA
>Mature_296_residues
MNLRSFQAFAEVVRQGGFSAAARTINATQSTVSKAVKQLEEELQLVLLDRAVSPPRLTAAGEIVYRRARAMLTEKDDMLA
ELGELRGLKRGLLRLGLPPIGSSVLFAPVFAAYTSLYPDIDIQLVEHGSKRLEELLLAGDVELAASLLPVEESFAWQDVR
CEPVVALLPASHPVSDDDGVTLREIANLPFILFESGFALNHIILSACQRHGFSPNVAVRSSQIDFIVELVAAGMGIGFLP
QMIAKQRHHPGVCIHRVKDADVNWHMAFVWRKGAFLSHAARAWLALCEGASTPAKA

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=284, Percent_Identity=26.056338028169, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=28.7671232876712, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693105, Length=294, Percent_Identity=23.1292517006803, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790399, Length=280, Percent_Identity=25.3571428571429, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787530, Length=285, Percent_Identity=22.1052631578947, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788887, Length=296, Percent_Identity=24.3243243243243, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=35.7723577235772, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787128, Length=129, Percent_Identity=29.4573643410853, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788748, Length=286, Percent_Identity=25.8741258741259, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788296, Length=248, Percent_Identity=25.4032258064516, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1789506, Length=288, Percent_Identity=21.875, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786401, Length=118, Percent_Identity=36.4406779661017, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=296, Percent_Identity=24.3243243243243, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367136, Length=262, Percent_Identity=23.2824427480916, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32294; Mature: 32294

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRSFQAFAEVVRQGGFSAAARTINATQSTVSKAVKQLEEELQLVLLDRAVSPPRLTAA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GEIVYRRARAMLTEKDDMLAELGELRGLKRGLLRLGLPPIGSSVLFAPVFAAYTSLYPDI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
DIQLVEHGSKRLEELLLAGDVELAASLLPVEESFAWQDVRCEPVVALLPASHPVSDDDGV
CHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCC
TLREIANLPFILFESGFALNHIILSACQRHGFSPNVAVRSSQIDFIVELVAAGMGIGFLP
CHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHCCCHHHHH
QMIAKQRHHPGVCIHRVKDADVNWHMAFVWRKGAFLSHAARAWLALCEGASTPAKA
HHHHHHCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNLRSFQAFAEVVRQGGFSAAARTINATQSTVSKAVKQLEEELQLVLLDRAVSPPRLTAA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
GEIVYRRARAMLTEKDDMLAELGELRGLKRGLLRLGLPPIGSSVLFAPVFAAYTSLYPDI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
DIQLVEHGSKRLEELLLAGDVELAASLLPVEESFAWQDVRCEPVVALLPASHPVSDDDGV
CHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCC
TLREIANLPFILFESGFALNHIILSACQRHGFSPNVAVRSSQIDFIVELVAAGMGIGFLP
CHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHCCCHHHHH
QMIAKQRHHPGVCIHRVKDADVNWHMAFVWRKGAFLSHAARAWLALCEGASTPAKA
HHHHHHCCCCCEEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]