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The map label for this gene is artJ [H]

Identifier: 23501202

GI number: 23501202

Start: 306203

End: 307045

Strand: Reverse

Name: artJ [H]

Synonym: BR0295

Alternate gene names: 23501202

Gene position: 307045-306203 (Counterclockwise)

Preceding gene: 23501204

Following gene: 23501201

Centisome position: 14.57

GC content: 58.13

Gene sequence:

>843_bases
ATGAAATCCTGTCTGTTGAGCCTGACGATAAGCATGTGCCTTTTCGCTCCTGCCGCCCATGCGGCGGGGGAAGAACCGGC
TGCACCGCATTTCTTCAACCAGAAAGAGCGCCTGCCCCTGCCTTCGCTGCAAGGGCTCAATCGTCTGCGCTTCATCACCA
CCGTCGATTTTCCGCCATTCAACAGGCTTAACGAACGCGGGCAGCTTTCCGGCTACAACATTGATCTGGCCAAGGCGCTT
TGCCAGCAGCTCAAGATCGAGGACATCTGCCAGATCGAGGCGGTGCCGTGGGCGGAACTGGAAGAGCGTGTGCTGGATGG
ACAGGCGGACGCGATCGTTGCGGGGTGGGAACCGACCGACAGGAACCGGGAAAAATTCACCTTCACCCGCAGCTATATGC
GCCTGCCCGCACGCTTTGCGACCTCCAATGCCAAAAGCTTTACAGAACCCGCTGCAAAGGCGACGCAGGACAAGCCCGTC
GGCGTGATTGCCGGGACGGCGCATGAAGCGCTTTTGAAGAACTATTTCCCACAGGCAAAACCCGTGCCCTACCCCAATCG
CGATGCCATGCTGACCGCATTGAAGGACGGCAAGATCGACACGGTTTTCGATGACGGCATGTCCCTCTCGGCCTGGCTCG
ACAGCGCAGAAGGCAGCGCCTGTTGTACTTTCACGGACGGGCCCTATCTGGCGCCTCAATATCTGGGTTCCGGCCTCAGC
ATCGCTGTTACCCGGCAAAATTCCGCCCTGGCAAGCGCCTTCAACAATGCCCTGCAAGCCCTGCAACAAGAAGGTAAGCT
GACCGAGCTTTACCTGAGATATTTCCCGGCAAGCTTTTATTAG

Upstream 100 bases:

>100_bases
ATATACGAATTATCAGGATGCCCGTTACTGCTATCGCCGATGGCGCTTGCATAGGCGCCGTTAACCCGGAATTATGGGGA
ATATTATAGGGTCGCCGGCG

Downstream 100 bases:

>100_bases
AGCATTTCCAGCCCCGCCTCGCACCCGCAAAGCACATAGCGGCGTCCACGGCCCGCACATGTGCGATTTTGACGCACTTT
AGCGAAATTCCGGCTGAAAC

Product: amino acid ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPFNRLNERGQLSGYNIDLAKAL
CQQLKIEDICQIEAVPWAELEERVLDGQADAIVAGWEPTDRNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPV
GVIAGTAHEALLKNYFPQAKPVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY

Sequences:

>Translated_280_residues
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPFNRLNERGQLSGYNIDLAKAL
CQQLKIEDICQIEAVPWAELEERVLDGQADAIVAGWEPTDRNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPV
GVIAGTAHEALLKNYFPQAKPVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY
>Mature_280_residues
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPFNRLNERGQLSGYNIDLAKAL
CQQLKIEDICQIEAVPWAELEERVLDGQADAIVAGWEPTDRNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPV
GVIAGTAHEALLKNYFPQAKPVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=231, Percent_Identity=30.7359307359307, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787088, Length=235, Percent_Identity=28.5106382978723, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788649, Length=234, Percent_Identity=23.0769230769231, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=25.531914893617, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=22.4215246636771, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30853; Mature: 30853

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCC
NRLNERGQLSGYNIDLAKALCQQLKIEDICQIEAVPWAELEERVLDGQADAIVAGWEPTD
HHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCC
RNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPVGVIAGTAHEALLKNYFPQAK
CCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCC
PVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
CCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCEECHHHHCCCCE
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY
EEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSCLLSLTISMCLFAPAAHAAGEEPAAPHFFNQKERLPLPSLQGLNRLRFITTVDFPPF
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCC
NRLNERGQLSGYNIDLAKALCQQLKIEDICQIEAVPWAELEERVLDGQADAIVAGWEPTD
HHHCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHCCCCCEEEECCCCCC
RNREKFTFTRSYMRLPARFATSNAKSFTEPAAKATQDKPVGVIAGTAHEALLKNYFPQAK
CCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCC
PVPYPNRDAMLTALKDGKIDTVFDDGMSLSAWLDSAEGSACCTFTDGPYLAPQYLGSGLS
CCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHCCCCCCEEEEECCCCEECHHHHCCCCE
IAVTRQNSALASAFNNALQALQQEGKLTELYLRYFPASFY
EEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]