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The map label for this gene is bepR

Identifier: 23501197

GI number: 23501197

Start: 300348

End: 300977

Strand: Reverse

Name: bepR

Synonym: BR0290

Alternate gene names: 23501197

Gene position: 300977-300348 (Counterclockwise)

Preceding gene: 23501200

Following gene: 23501196

Centisome position: 14.28

GC content: 56.35

Gene sequence:

>630_bases
ATGCGCAGAACAAAAGCCGAGGCCGCGGAAACCCGTGAAGCCATTCTTCTGGCAGCCGAACAGGTGTTCCTCGAACGCGG
CGTGAACCAGTCCACACTGACGGAAATCGCATGTTACGCAGGCGTGACGCGCGGCGCGATCTATTTTCATTTTGAAGACA
AGCTCGATATTTTCCAGTCCATCATTGGCCGGGCGCGCTTTCCGCAGGAAGAGATCATGCTTCAGGCGGCGCGCTTCGAC
CATCCCAATCCGCTCCATATTCTCGAACAATCCATCGTCGCCGCGCTGGAGCTTTTCGCCACCGATGAAAGACAGCAGGT
CGTCTTCACCATCATCAACCAGCGTTGCGAATATGTGGGCGAGATGGCTCCCGTCATCGACCGGCTGAAGGAAATGCGCA
GTGATGTGCTGGCGCTTTTCATCGGCCTTTTGAAAGTGGCCGAGCGGCGCGGCGAGCTTGCAAGCGAATGGAGCGCCGAA
ACGGCGGCGCAAATTCTGCTCGCCATGGTGGGTGGTTTTCTCAATGAATGGTTACACGGTGAAAAAGGTTTCGATCTCAT
CATCCATGGCAGCCGTGTGATTTCCACAGTGATCCAGTCGCTGCGCGCACCCGCAAATATCCCGCAGTAG

Upstream 100 bases:

>100_bases
GGGTGCTATAGTCAATTGCTTAATGCCATCAATCACGAATGAGTTATCGAAATTTGTGGGTTGAAACCAGAAATGGATTT
CAATTCATCCGGGAATCATC

Downstream 100 bases:

>100_bases
AGCATTTTCGAGCCAAACATGGGAAACGCCGATGCGTCGGATAATGCGCCAAAACAAAGAGTTAGGCTGTAGTGACGATT
CTGTTAAAATAGGAACCACT

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MRRTKAEAAETREAILLAAEQVFLERGVNQSTLTEIACYAGVTRGAIYFHFEDKLDIFQSIIGRARFPQEEIMLQAARFD
HPNPLHILEQSIVAALELFATDERQQVVFTIINQRCEYVGEMAPVIDRLKEMRSDVLALFIGLLKVAERRGELASEWSAE
TAAQILLAMVGGFLNEWLHGEKGFDLIIHGSRVISTVIQSLRAPANIPQ

Sequences:

>Translated_209_residues
MRRTKAEAAETREAILLAAEQVFLERGVNQSTLTEIACYAGVTRGAIYFHFEDKLDIFQSIIGRARFPQEEIMLQAARFD
HPNPLHILEQSIVAALELFATDERQQVVFTIINQRCEYVGEMAPVIDRLKEMRSDVLALFIGLLKVAERRGELASEWSAE
TAAQILLAMVGGFLNEWLHGEKGFDLIIHGSRVISTVIQSLRAPANIPQ
>Mature_209_residues
MRRTKAEAAETREAILLAAEQVFLERGVNQSTLTEIACYAGVTRGAIYFHFEDKLDIFQSIIGRARFPQEEIMLQAARFD
HPNPLHILEQSIVAALELFATDERQQVVFTIINQRCEYVGEMAPVIDRLKEMRSDVLALFIGLLKVAERRGELASEWSAE
TAAQILLAMVGGFLNEWLHGEKGFDLIIHGSRVISTVIQSLRAPANIPQ

Specific function: Represses expression of bepDE

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786669, Length=213, Percent_Identity=33.8028169014084, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789664, Length=211, Percent_Identity=28.9099526066351, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BEPR_BRUSU (Q8G2M8)

Other databases:

- EMBL:   AE014291
- RefSeq:   NP_697324.1
- ProteinModelPortal:   Q8G2M8
- SMR:   Q8G2M8
- GeneID:   1165951
- GenomeReviews:   AE014291_GR
- KEGG:   bms:BR0290
- NMPDR:   fig|204722.1.peg.281
- TIGR:   BR0290
- HOGENOM:   HBG701145
- OMA:   TRRINEI
- PhylomeDB:   Q8G2M8
- ProtClustDB:   CLSK898495
- BioCyc:   BSUI204722:BR_0290-MONOMER
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR013572
- InterPro:   IPR001647
- Gene3D:   G3DSA:1.10.10.60
- Gene3D:   G3DSA:1.10.357.10
- PRINTS:   PR00455

Pfam domain/function: PF08361 TetR_C_2; PF00440 TetR_N; SSF46689 Homeodomain_like; SSF48498 TetR_like_C

EC number: NA

Molecular weight: Translated: 23478; Mature: 23478

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01081 HTH_TETR_1; PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKAEAAETREAILLAAEQVFLERGVNQSTLTEIACYAGVTRGAIYFHFEDKLDIFQS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECHHHHHHHH
IIGRARFPQEEIMLQAARFDHPNPLHILEQSIVAALELFATDERQQVVFTIINQRCEYVG
HHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EMAPVIDRLKEMRSDVLALFIGLLKVAERRGELASEWSAETAAQILLAMVGGFLNEWLHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EKGFDLIIHGSRVISTVIQSLRAPANIPQ
CCCCEEEEECHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRRTKAEAAETREAILLAAEQVFLERGVNQSTLTEIACYAGVTRGAIYFHFEDKLDIFQS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEEEECHHHHHHHH
IIGRARFPQEEIMLQAARFDHPNPLHILEQSIVAALELFATDERQQVVFTIINQRCEYVG
HHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
EMAPVIDRLKEMRSDVLALFIGLLKVAERRGELASEWSAETAAQILLAMVGGFLNEWLHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EKGFDLIIHGSRVISTVIQSLRAPANIPQ
CCCCEEEEECHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12271122