Click here to switch to the map view.

The map label for this gene is thiD [H]

Identifier: 23501125

GI number: 23501125

Start: 226984

End: 227859

Strand: Reverse

Name: thiD [H]

Synonym: BR0218

Alternate gene names: 23501125

Gene position: 227859-226984 (Counterclockwise)

Preceding gene: 23501128

Following gene: 23501124

Centisome position: 10.81

GC content: 62.79

Gene sequence:

>876_bases
ATGGACCAGAAACAGAGTTTCTCCCCAACCAATCCAACCGATAACCATGCGGCATCCGTGCCCATATGCGTCACCATTGC
GGGGTCCGACTCCAGCGGCGGCGCGGGCATTCAGGCCGACCTCAAGGCGTTTTCAGCGCTTGGCGTCTATGGCGCAAGCG
TGATTGCCGCCATCACCGTGCAGAACACTCGCACCGTCAGCGCCGTGCATGATGTGCCGCCGCAGATCGTGGCGGGCCAG
ATAGACGCCGTCTTCAGCGATCTCGATGTCGCCGCCGTCAAGATCGGCATGGTTTCCGTGCCAGAAACCATCGAAGCCAT
TGCGCACGGCCTTGCACATTTTTCCGGTCCCATCGTGCTCGACCCGGTGACGGTTGCAAAATCCGGCGACCGGCTGTTGA
GCGAGACCGCGATTTCATCCCTGCGTAAAAAGCTGCTGCCGCTTGCAACACTGCTCACCCCCAACCGGCCGGAAGCCGCC
TGCCTGCTCGACACAGGCACAGCCGGGAGTGATGCCGAGGCGGAAGAACAGGGCCGCGCCCTGCTCGACCTCGGTGCCAA
GGCCGTCCTGATGAAAGGCGGCCATGCGGAAGGGGCGATCTGCACCGATCTTCTGATCCGCCGCGACCGGCCGACCCTGC
GCATCGAAGCGCCGCGCATTCTCACCAACAACACGCATGGCACCGGCTGCACGCTTTCATCCGCCATTGCTGCCGGGCTT
TCGCGCAACCTTTCGCTCGAAACAGCGCTCGCGCAGGCCCATGCCTATCTGCAAGGCGCAATCCGCGCGGCTGACAGTCT
GAAGGTCGGTCAGGGCCATGGGCCGGTGCATCATTTTCACGCCCTTTGGAAACAGGCAGAAAGGGAGGCGATCTGA

Upstream 100 bases:

>100_bases
GAGTTTGGCGCGTCGCGGACGGGGTTCATTCCCCATCGGGGCTTATGCTCCATCAAGGTTCATGACGTCTCATTTTCCGT
TCGGAATGAGAGACGTCGAA

Downstream 100 bases:

>100_bases
TGCGCGTGACAATCATCGGAGCGGGTGTCGCTGGCCTTGCCACCGCCGCGGAATTTACCGATCAGGGCCATGCCGTCACC
GTCATCGCCAAAAGTGCCGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MDQKQSFSPTNPTDNHAASVPICVTIAGSDSSGGAGIQADLKAFSALGVYGASVIAAITVQNTRTVSAVHDVPPQIVAGQ
IDAVFSDLDVAAVKIGMVSVPETIEAIAHGLAHFSGPIVLDPVTVAKSGDRLLSETAISSLRKKLLPLATLLTPNRPEAA
CLLDTGTAGSDAEAEEQGRALLDLGAKAVLMKGGHAEGAICTDLLIRRDRPTLRIEAPRILTNNTHGTGCTLSSAIAAGL
SRNLSLETALAQAHAYLQGAIRAADSLKVGQGHGPVHHFHALWKQAEREAI

Sequences:

>Translated_291_residues
MDQKQSFSPTNPTDNHAASVPICVTIAGSDSSGGAGIQADLKAFSALGVYGASVIAAITVQNTRTVSAVHDVPPQIVAGQ
IDAVFSDLDVAAVKIGMVSVPETIEAIAHGLAHFSGPIVLDPVTVAKSGDRLLSETAISSLRKKLLPLATLLTPNRPEAA
CLLDTGTAGSDAEAEEQGRALLDLGAKAVLMKGGHAEGAICTDLLIRRDRPTLRIEAPRILTNNTHGTGCTLSSAIAAGL
SRNLSLETALAQAHAYLQGAIRAADSLKVGQGHGPVHHFHALWKQAEREAI
>Mature_291_residues
MDQKQSFSPTNPTDNHAASVPICVTIAGSDSSGGAGIQADLKAFSALGVYGASVIAAITVQNTRTVSAVHDVPPQIVAGQ
IDAVFSDLDVAAVKIGMVSVPETIEAIAHGLAHFSGPIVLDPVTVAKSGDRLLSETAISSLRKKLLPLATLLTPNRPEAA
CLLDTGTAGSDAEAEEQGRALLDLGAKAVLMKGGHAEGAICTDLLIRRDRPTLRIEAPRILTNNTHGTGCTLSSAIAAGL
SRNLSLETALAQAHAYLQGAIRAADSLKVGQGHGPVHHFHALWKQAEREAI

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=261, Percent_Identity=52.4904214559387, Blast_Score=263, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6325378, Length=278, Percent_Identity=30.5755395683453, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324997, Length=283, Percent_Identity=32.1554770318021, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324517, Length=279, Percent_Identity=31.1827956989247, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 30056; Mature: 30056

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQKQSFSPTNPTDNHAASVPICVTIAGSDSSGGAGIQADLKAFSALGVYGASVIAAITV
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEE
QNTRTVSAVHDVPPQIVAGQIDAVFSDLDVAAVKIGMVSVPETIEAIAHGLAHFSGPIVL
CCCEEEEHHHCCCHHHHHHHHHHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHHCCCEEE
DPVTVAKSGDRLLSETAISSLRKKLLPLATLLTPNRPEAACLLDTGTAGSDAEAEEQGRA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHCCC
LLDLGAKAVLMKGGHAEGAICTDLLIRRDRPTLRIEAPRILTNNTHGTGCTLSSAIAAGL
HHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCHHHHHHHHHH
SRNLSLETALAQAHAYLQGAIRAADSLKVGQGHGPVHHFHALWKQAEREAI
HCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDQKQSFSPTNPTDNHAASVPICVTIAGSDSSGGAGIQADLKAFSALGVYGASVIAAITV
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEE
QNTRTVSAVHDVPPQIVAGQIDAVFSDLDVAAVKIGMVSVPETIEAIAHGLAHFSGPIVL
CCCEEEEHHHCCCHHHHHHHHHHHHHCCCCHHHEEECCCCHHHHHHHHHHHHHHCCCEEE
DPVTVAKSGDRLLSETAISSLRKKLLPLATLLTPNRPEAACLLDTGTAGSDAEAEEQGRA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHCCC
LLDLGAKAVLMKGGHAEGAICTDLLIRRDRPTLRIEAPRILTNNTHGTGCTLSSAIAAGL
HHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCHHHHHHHHHH
SRNLSLETALAQAHAYLQGAIRAADSLKVGQGHGPVHHFHALWKQAEREAI
HCCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431; 8246891 [H]