Click here to switch to the map view.

The map label for this gene is lspA

Identifier: 23501060

GI number: 23501060

Start: 167187

End: 167669

Strand: Reverse

Name: lspA

Synonym: BR0149

Alternate gene names: 23501060

Gene position: 167669-167187 (Counterclockwise)

Preceding gene: 23501061

Following gene: 23501059

Centisome position: 7.95

GC content: 52.8

Gene sequence:

>483_bases
ATGAAGCGTCACGCAGTCTGGTCCTCGCTTTTCGTCGTCATTCTTGCAGTCCTGATTGACCAGGGCATCAAATATCTGGT
CGAAAGCCGCATGTTCTATGGGCAGCAGATCGATCTTCTGCCCTTCCTCGCCCTTTTTCGCACGCATAATGAAGGCATCG
CCTTTTCCATGCTGGCATGGCTGCACGATGGCGGCCTGATCGCCATCACGCTCGCCGTCATCGCCTTTGTTCTCTATCTG
TGGTGGACCAATGCGCCGGAGCGTGTTTTTGCGCGCTATGGCTTCGCACTCGTGATCGGTGGGGCCATCGGCAATCTCAT
TGACCGCGTGATGCATGGCTATGTGGTTGATTATGTTCTTTTTCACCTGCCAACATGGTCTTTTGCAGTATTCAATCTTG
CAGATGCATTCATCACGATTGGAGCAGGGCTGATTATTCTGGAAGAGTTTCTCGGCTGGCGGCGCGAACGAATCTCACAC
TGA

Upstream 100 bases:

>100_bases
CAGGCAGGCCGCGCCGATTCGCTCAATCTGGCCATTGCCACAGGTGTGATGCTCTATGAAATCCGCCGTGAGGCACTCAC
CCTCGATGAAAGGGGATAGG

Downstream 100 bases:

>100_bases
AACCGGAAGCAAAGGTTGCCGCTTGATCGGCTGTGCCCTTTCACATATTTTATGGAAGAAATTTTCAGTGTTTTTTTAAG
GAGATAGCGCAGCCCAATCC

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKRHAVWSSLFVVILAVLIDQGIKYLVESRMFYGQQIDLLPFLALFRTHNEGIAFSMLAWLHDGGLIAITLAVIAFVLYL
WWTNAPERVFARYGFALVIGGAIGNLIDRVMHGYVVDYVLFHLPTWSFAVFNLADAFITIGAGLIILEEFLGWRRERISH

Sequences:

>Translated_160_residues
MKRHAVWSSLFVVILAVLIDQGIKYLVESRMFYGQQIDLLPFLALFRTHNEGIAFSMLAWLHDGGLIAITLAVIAFVLYL
WWTNAPERVFARYGFALVIGGAIGNLIDRVMHGYVVDYVLFHLPTWSFAVFNLADAFITIGAGLIILEEFLGWRRERISH
>Mature_160_residues
MKRHAVWSSLFVVILAVLIDQGIKYLVESRMFYGQQIDLLPFLALFRTHNEGIAFSMLAWLHDGGLIAITLAVIAFVLYL
WWTNAPERVFARYGFALVIGGAIGNLIDRVMHGYVVDYVLFHLPTWSFAVFNLADAFITIGAGLIILEEFLGWRRERISH

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=155, Percent_Identity=41.2903225806452, Blast_Score=101, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_BRUA1 (B2S8E3)

Other databases:

- EMBL:   CP000887
- RefSeq:   YP_001934170.1
- GeneID:   6328086
- GenomeReviews:   CP000887_GR
- KEGG:   bmc:BAbS19_I01400
- HOGENOM:   HBG724422
- OMA:   ADSSGWQ
- ProtClustDB:   PRK14795
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 18230; Mature: 18230

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 108-108 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

HASH(0x91f9ed4)-; HASH(0xb00caec)-; HASH(0xbeaf354)-; HASH(0xc0108a0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHAVWSSLFVVILAVLIDQGIKYLVESRMFYGQQIDLLPFLALFRTHNEGIAFSMLAW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LHDGGLIAITLAVIAFVLYLWWTNAPERVFARYGFALVIGGAIGNLIDRVMHGYVVDYVL
HHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHLPTWSFAVFNLADAFITIGAGLIILEEFLGWRRERISH
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MKRHAVWSSLFVVILAVLIDQGIKYLVESRMFYGQQIDLLPFLALFRTHNEGIAFSMLAW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHH
LHDGGLIAITLAVIAFVLYLWWTNAPERVFARYGFALVIGGAIGNLIDRVMHGYVVDYVL
HHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHLPTWSFAVFNLADAFITIGAGLIILEEFLGWRRERISH
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA