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The map label for this gene is pbpF [H]

Identifier: 23501031

GI number: 23501031

Start: 127951

End: 130242

Strand: Reverse

Name: pbpF [H]

Synonym: BR0117

Alternate gene names: 23501031

Gene position: 130242-127951 (Counterclockwise)

Preceding gene: 23501032

Following gene: 23501030

Centisome position: 6.18

GC content: 59.95

Gene sequence:

>2292_bases
ATGGCATCGCGAGACGACAGGAACAGACGCATCGAACCTTCCTTCGGTGGGGAAGAGAAGCGGGAAGATGAGGTCTTTCA
GGTCGATGAGGAGGATCGCATGGCTCGCTCGCAACAGCGCAGGCAAAAGCAATCCGCAGATCGTGCTCCCCGCTCTTCGC
GGGGCGGTAAAAGAAAGCAACGCGGCTTTTTCGGTTTTCTACGCCGTTCGATCTACTGGTTTCTCGTGCTGGGCCTTTGG
GGCGGCATCGCCTTTGCTGGCCTTCTGGTCTATTTCGCGGCCAAGATGCCGCCGACGACCGCCTGGGCCATTCCTGACAG
GCCACCCAATGTGCGCATTGTCGATGTGAACAGCAATCTCATCGCCAACCGTGGCACGACGGGCGGCGAGGCCGTGAGCC
TGCATGAAATGTCGCCCTATATTCCCCAGGCCGTTATCGCCATTGAGGACCGCCGCTTCAATTCGCATTTCGGTATCGAC
CCGATCGGGCTTGCGCGCGCTATCGTAACCAATGTGGTTTCGGGCCGCGCGGTGCAGGGCGGCTCCACGCTGACACAGCA
GCTTGCCAAGAATCTCTTTCTTTCGCCTGACCGCACGCTGGAGCGCAAGGTGCAGGAAGTGATGCTCGCCCTTTGGCTGG
AGCATAAATATACCAAGGACCAGATATTGGAGATGTATCTGAACCGGGTTTATCTGGGTTCGGGCGCTTTCGGCGTCGAT
GCGGCATCGCGGCGCTATTTCAACAAATCGGCCAAGGACGTGAACCTGATGGAAGCGGCAACGCTTGCAGGGCTCCTGAA
AGCGCCTTCGCGCCTCTCCCCTGCCCGCGACCCGGAAGCCGCCGCAGCGCGCGCCAGGCTGGTGCTGGGCGCCATGCGCG
AGGAAGGCATGATCGACGACAGGCAGGTGGCGATTGCCGAAAGCGAACCACCGACGCGCGCGCCTTCCTACTGGCAGGGT
TCGGAAAACTATGTGGCCGACAAGGTTGTCGCCGAATTGCCCGAACTGATCGGCGAAGCAAAGGATGACATCATCGTCCA
GACGACCATCGATCTTAACCTCCAGCGCGCTGGCGAAGAGGCGATCAAGGATCAGATCGAGCAGAACGGCAAAAAGATGA
ATGCGAGCCAGGGCGCGCTTGTTTCCATCGATAATACGGGCGCGGTGCGCGCCATGGTGGGCGGGGTGGACTATGCGGCC
AGCCAGTTTGACCGCGTGACCGATGCGCATCGCCAACCGGCTTCCTCGTTCAAGCCCTTTGTCTATCTTTCTGCGCTGGA
ACAGGGCCGGACACCGGATTCCGTGCGCAATGACGCGCCTGTGCGCATCGGCAAATGGACACCGAGTAATGATAACGGCA
AGTATATGGGGCCAGTGACGCTCGCCACCGCACTTTCCCATTCGCTGAACTCGGTCGCAGCCCAGCTTGTGATGGAAGTC
GGCCCCCAAACCGTGATCGACACGGCCCATCGCCTCGGTGTGCAGTCGAAGCTTGAACCCAATGCTTCGCTGGCGCTAGG
CACGTCGGAAGTGACACTTCTTGAATTAACCGACGCCTATGTTCCCTTCGCCAATGGCGGCTATCGCGCGCCGGTCTATT
TCATTACCAAGATGACGGATTCCGATGGAAACGTGCTTTACCAGAAGGAAGATGGTGTCGGCCCGCGCGTGATCGACGAG
CGCAATGTCGGCATGATGAACGCCATGCTGCGCCGCACCGTGGAAGATGGCACCGCCAAACGCGCCGCCTTCGGCTGGCC
GGCCGCAGGCAAAACCGGCACCAGCCAGAATTTCCGCGATGCCTGGTTCATCGGCTACACAGCCAATCTTACGACCGGCG
TTTGGTTCGGCAATGACGACGGCAAGGGCATGAAGCGCGTTTTTGGCGCAACACTTCCCGTGGCGGCCTGGAAAAGCTTC
ATGAAGGAAGCACATAAGGGTGTTCCCATCGCCGAGCTTCCCGGCCATTACGATATCCAGAACGTGCTGCCCGGCGGCAG
CGACGGCATAGACCCCAATGCGCCTTATGATCCCGGCATGATGCAGCAGGATAATTATGGCAACCAGCCGATGGCAGGCA
CCGGCGACGATGGCTACTGGCCACCGGCGCCGGATTTGCCCGGCCGAAGCGGTATCCAGCAGGGCGAAACCGGACAGCCC
CTTGCCTCTTCCGCCAGGAATAACGGCGATTACCGGCCCATGCCTGCGGGCAATGTGGGCGAACAGCCGCAAGCCCAGCC
TGCGAGAAAATCCACCACCCTGCTCGATATCATCATGGGTAATTCACAGTAA

Upstream 100 bases:

>100_bases
CTTCAGGGCGATAGCGAACGGTGGGGCACCGCTCTGTGCGAGCAGATCGAAACACGCTCCGGCATGAAAAACAAGCGCGT
GGGGCGCGGATCAGGCAGAC

Downstream 100 bases:

>100_bases
AAACAGGAGGGACTGGCAAGCGGCCAGTCCCTTCATGGTGCTTTCATTCCCTTGCCATGCTGAATCCCCCTCGAAATCTT
GAGCGGCATGGTCTATTCAA

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MASRDDRNRRIEPSFGGEEKREDEVFQVDEEDRMARSQQRRQKQSADRAPRSSRGGKRKQRGFFGFLRRSIYWFLVLGLW
GGIAFAGLLVYFAAKMPPTTAWAIPDRPPNVRIVDVNSNLIANRGTTGGEAVSLHEMSPYIPQAVIAIEDRRFNSHFGID
PIGLARAIVTNVVSGRAVQGGSTLTQQLAKNLFLSPDRTLERKVQEVMLALWLEHKYTKDQILEMYLNRVYLGSGAFGVD
AASRRYFNKSAKDVNLMEAATLAGLLKAPSRLSPARDPEAAAARARLVLGAMREEGMIDDRQVAIAESEPPTRAPSYWQG
SENYVADKVVAELPELIGEAKDDIIVQTTIDLNLQRAGEEAIKDQIEQNGKKMNASQGALVSIDNTGAVRAMVGGVDYAA
SQFDRVTDAHRQPASSFKPFVYLSALEQGRTPDSVRNDAPVRIGKWTPSNDNGKYMGPVTLATALSHSLNSVAAQLVMEV
GPQTVIDTAHRLGVQSKLEPNASLALGTSEVTLLELTDAYVPFANGGYRAPVYFITKMTDSDGNVLYQKEDGVGPRVIDE
RNVGMMNAMLRRTVEDGTAKRAAFGWPAAGKTGTSQNFRDAWFIGYTANLTTGVWFGNDDGKGMKRVFGATLPVAAWKSF
MKEAHKGVPIAELPGHYDIQNVLPGGSDGIDPNAPYDPGMMQQDNYGNQPMAGTGDDGYWPPAPDLPGRSGIQQGETGQP
LASSARNNGDYRPMPAGNVGEQPQAQPARKSTTLLDIIMGNSQ

Sequences:

>Translated_763_residues
MASRDDRNRRIEPSFGGEEKREDEVFQVDEEDRMARSQQRRQKQSADRAPRSSRGGKRKQRGFFGFLRRSIYWFLVLGLW
GGIAFAGLLVYFAAKMPPTTAWAIPDRPPNVRIVDVNSNLIANRGTTGGEAVSLHEMSPYIPQAVIAIEDRRFNSHFGID
PIGLARAIVTNVVSGRAVQGGSTLTQQLAKNLFLSPDRTLERKVQEVMLALWLEHKYTKDQILEMYLNRVYLGSGAFGVD
AASRRYFNKSAKDVNLMEAATLAGLLKAPSRLSPARDPEAAAARARLVLGAMREEGMIDDRQVAIAESEPPTRAPSYWQG
SENYVADKVVAELPELIGEAKDDIIVQTTIDLNLQRAGEEAIKDQIEQNGKKMNASQGALVSIDNTGAVRAMVGGVDYAA
SQFDRVTDAHRQPASSFKPFVYLSALEQGRTPDSVRNDAPVRIGKWTPSNDNGKYMGPVTLATALSHSLNSVAAQLVMEV
GPQTVIDTAHRLGVQSKLEPNASLALGTSEVTLLELTDAYVPFANGGYRAPVYFITKMTDSDGNVLYQKEDGVGPRVIDE
RNVGMMNAMLRRTVEDGTAKRAAFGWPAAGKTGTSQNFRDAWFIGYTANLTTGVWFGNDDGKGMKRVFGATLPVAAWKSF
MKEAHKGVPIAELPGHYDIQNVLPGGSDGIDPNAPYDPGMMQQDNYGNQPMAGTGDDGYWPPAPDLPGRSGIQQGETGQP
LASSARNNGDYRPMPAGNVGEQPQAQPARKSTTLLDIIMGNSQ
>Mature_762_residues
ASRDDRNRRIEPSFGGEEKREDEVFQVDEEDRMARSQQRRQKQSADRAPRSSRGGKRKQRGFFGFLRRSIYWFLVLGLWG
GIAFAGLLVYFAAKMPPTTAWAIPDRPPNVRIVDVNSNLIANRGTTGGEAVSLHEMSPYIPQAVIAIEDRRFNSHFGIDP
IGLARAIVTNVVSGRAVQGGSTLTQQLAKNLFLSPDRTLERKVQEVMLALWLEHKYTKDQILEMYLNRVYLGSGAFGVDA
ASRRYFNKSAKDVNLMEAATLAGLLKAPSRLSPARDPEAAAARARLVLGAMREEGMIDDRQVAIAESEPPTRAPSYWQGS
ENYVADKVVAELPELIGEAKDDIIVQTTIDLNLQRAGEEAIKDQIEQNGKKMNASQGALVSIDNTGAVRAMVGGVDYAAS
QFDRVTDAHRQPASSFKPFVYLSALEQGRTPDSVRNDAPVRIGKWTPSNDNGKYMGPVTLATALSHSLNSVAAQLVMEVG
PQTVIDTAHRLGVQSKLEPNASLALGTSEVTLLELTDAYVPFANGGYRAPVYFITKMTDSDGNVLYQKEDGVGPRVIDER
NVGMMNAMLRRTVEDGTAKRAAFGWPAAGKTGTSQNFRDAWFIGYTANLTTGVWFGNDDGKGMKRVFGATLPVAAWKSFM
KEAHKGVPIAELPGHYDIQNVLPGGSDGIDPNAPYDPGMMQQDNYGNQPMAGTGDDGYWPPAPDLPGRSGIQQGETGQPL
ASSARNNGDYRPMPAGNVGEQPQAQPARKSTTLLDIIMGNSQ

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=585, Percent_Identity=31.7948717948718, Blast_Score=233, Evalue=4e-62,
Organism=Escherichia coli, GI1788867, Length=581, Percent_Identity=32.8743545611015, Blast_Score=222, Evalue=6e-59,
Organism=Escherichia coli, GI87082258, Length=307, Percent_Identity=37.4592833876222, Blast_Score=190, Evalue=4e-49,
Organism=Escherichia coli, GI1789601, Length=236, Percent_Identity=33.8983050847458, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 83153; Mature: 83021

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASRDDRNRRIEPSFGGEEKREDEVFQVDEEDRMARSQQRRQKQSADRAPRSSRGGKRKQ
CCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
RGFFGFLRRSIYWFLVLGLWGGIAFAGLLVYFAAKMPPTTAWAIPDRPPNVRIVDVNSNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECCCCE
IANRGTTGGEAVSLHEMSPYIPQAVIAIEDRRFNSHFGIDPIGLARAIVTNVVSGRAVQG
EECCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEECC
GSTLTQQLAKNLFLSPDRTLERKVQEVMLALWLEHKYTKDQILEMYLNRVYLGSGAFGVD
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCC
AASRRYFNKSAKDVNLMEAATLAGLLKAPSRLSPARDPEAAAARARLVLGAMREEGMIDD
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
RQVAIAESEPPTRAPSYWQGSENYVADKVVAELPELIGEAKDDIIVQTTIDLNLQRAGEE
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHH
AIKDQIEQNGKKMNASQGALVSIDNTGAVRAMVGGVDYAASQFDRVTDAHRQPASSFKPF
HHHHHHHHCCCEECCCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCE
VYLSALEQGRTPDSVRNDAPVRIGKWTPSNDNGKYMGPVTLATALSHSLNSVAAQLVMEV
EEEEHHHCCCCCCHHCCCCCEEEECCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHH
GPQTVIDTAHRLGVQSKLEPNASLALGTSEVTLLELTDAYVPFANGGYRAPVYFITKMTD
CCHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEC
SDGNVLYQKEDGVGPRVIDERNVGMMNAMLRRTVEDGTAKRAAFGWPAAGKTGTSQNFRD
CCCCEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCC
AWFIGYTANLTTGVWFGNDDGKGMKRVFGATLPVAAWKSFMKEAHKGVPIAELPGHYDIQ
EEEEEEECCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHH
NVLPGGSDGIDPNAPYDPGMMQQDNYGNQPMAGTGDDGYWPPAPDLPGRSGIQQGETGQP
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
LASSARNNGDYRPMPAGNVGEQPQAQPARKSTTLLDIIMGNSQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCC
>Mature Secondary Structure 
ASRDDRNRRIEPSFGGEEKREDEVFQVDEEDRMARSQQRRQKQSADRAPRSSRGGKRKQ
CCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
RGFFGFLRRSIYWFLVLGLWGGIAFAGLLVYFAAKMPPTTAWAIPDRPPNVRIVDVNSNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEECCCCE
IANRGTTGGEAVSLHEMSPYIPQAVIAIEDRRFNSHFGIDPIGLARAIVTNVVSGRAVQG
EECCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCEECC
GSTLTQQLAKNLFLSPDRTLERKVQEVMLALWLEHKYTKDQILEMYLNRVYLGSGAFGVD
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEECCCCCCCC
AASRRYFNKSAKDVNLMEAATLAGLLKAPSRLSPARDPEAAAARARLVLGAMREEGMIDD
HHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
RQVAIAESEPPTRAPSYWQGSENYVADKVVAELPELIGEAKDDIIVQTTIDLNLQRAGEE
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHH
AIKDQIEQNGKKMNASQGALVSIDNTGAVRAMVGGVDYAASQFDRVTDAHRQPASSFKPF
HHHHHHHHCCCEECCCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHCCCHHHCCCE
VYLSALEQGRTPDSVRNDAPVRIGKWTPSNDNGKYMGPVTLATALSHSLNSVAAQLVMEV
EEEEHHHCCCCCCHHCCCCCEEEECCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHH
GPQTVIDTAHRLGVQSKLEPNASLALGTSEVTLLELTDAYVPFANGGYRAPVYFITKMTD
CCHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEEEEC
SDGNVLYQKEDGVGPRVIDERNVGMMNAMLRRTVEDGTAKRAAFGWPAAGKTGTSQNFRD
CCCCEEEEECCCCCCCEECCCCCHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCC
AWFIGYTANLTTGVWFGNDDGKGMKRVFGATLPVAAWKSFMKEAHKGVPIAELPGHYDIQ
EEEEEEECCCEEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCHH
NVLPGGSDGIDPNAPYDPGMMQQDNYGNQPMAGTGDDGYWPPAPDLPGRSGIQQGETGQP
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
LASSARNNGDYRPMPAGNVGEQPQAQPARKSTTLLDIIMGNSQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]