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The map label for this gene is argG [H]

Identifier: 23500989

GI number: 23500989

Start: 82025

End: 83245

Strand: Reverse

Name: argG [H]

Synonym: BR0074

Alternate gene names: 23500989

Gene position: 83245-82025 (Counterclockwise)

Preceding gene: 23500990

Following gene: 23500986

Centisome position: 3.95

GC content: 58.15

Gene sequence:

>1221_bases
ATGAGCAAGTGGAAAGACGTTAAGAAAGTCGTTCTCGCCTATTCGGGCGGCCTCGACACCTCGATCATCCTGAAGTGGCT
TCAGACGGAACTGGGCGCGGAAGTCGTGACCTTTACCGCTGATCTCGGCCAGGGCGAGGAACTTGAACCGGCCCGCAAGA
AAGCGGAAATGCTGGGCATCAAGGAAATCTTCATTGAGGACGTGCGCGAAGAATTCGTGCGCGATTTCGTCTTCCCGATG
TTCCGCGCCAATGCCGTCTATGAAGGCGTCTATCTGCTCGGCACATCGATTGCCCGCCCACTGATCTCCAAGCATCTGAT
CGACATCGCCAAAAAAACCGGCGCCGACGCCATCGCCCATGGCGCGACCGGCAAGGGCAACGACCAGGTTCGCTTCGAGC
TTTCGGCCTATGCGCTGAACCCGGACATCAAGATCATCGCTCCATGGCGCGACTGGTCGTTCAAGAGCCGCACGCAGCTT
CTCGAATTTGCCGAACAGCACCAGATTCCGGTTGCCAAGGACAAAAAGGGCGAAGCACCGTTCTCCGTCGATGCCAACCT
GCTGCACTCCTCGTCGGAGGGCAAGGTTCTGGAAGACCCATCGCAGGAAGCGCCGGAATATGTGCATATGCGCACTATTT
CGCCGGAAACCGCGCCCGACAAGGCAACGATCATCAAGATCGGCTTTGAAAAGGGCGATGCCGTTTCAATCAATGGCGAG
CGCCTGTCGCCAGCGACCCTTCTTGCCAAGCTCAACGACTATGGCCGCGACAATGGCATTGGCCGTCTCGACCTCGTGGA
AAACCGCTTCGTCGGCATGAAGTCGCGCGGCGTCTACGAAACGCCGGGCGGTACGATCCTGCTTGCAGCGCACCGCGCCA
TTGAATCGATCACGCTCGACCGTGGTGCGGCGCACCTGAAAGACGAGCTGATGCCGCGCTATGCGGAACTGATCTATTAT
GGCTTCTGGTTCTCGCCGGAACGCGAAATGCTGCAAGCGGCCATCGATCACAGCCAGCACCATGTCGAAGGCGAAGTCAC
GCTCAAGCTCTACAAGGGCAATGTGATGGTGATCGGTCGCGAATCGGCCAAGTCGCTCTATTCCGACAAGCTCGTGACCT
TTGAAGACGATCAGGGCGCCTACGACCAGAAGGATGCGGCAGGCTTCATCAAGCTCAATGCGCTGCGTCTGCGCACACTG
GCTGCACGCGACCGCAAATAA

Upstream 100 bases:

>100_bases
CCATCCTGCTTGCACGCGGCTTCGCAACTGATAAAAGACTTGCGGATATATCTGTTGGAGCTCCCGCTCCAGCCACCACC
GCATCAGACGGAAGTGAACT

Downstream 100 bases:

>100_bases
ATATAGCTCATTTGGACAAAAGCCCGCTCTCCGGCGGGCTTTTGTTTTCAAAGCGCATCCCGAAAAGTGTGATGCGGTTC
AGATTGCAGCAAGCGCGTGG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase 2 [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MSKWKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIFIEDVREEFVRDFVFPM
FRANAVYEGVYLLGTSIARPLISKHLIDIAKKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTQL
LEFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPSQEAPEYVHMRTISPETAPDKATIIKIGFEKGDAVSINGE
RLSPATLLAKLNDYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDELMPRYAELIYY
GFWFSPEREMLQAAIDHSQHHVEGEVTLKLYKGNVMVIGRESAKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTL
AARDRK

Sequences:

>Translated_406_residues
MSKWKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIFIEDVREEFVRDFVFPM
FRANAVYEGVYLLGTSIARPLISKHLIDIAKKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTQL
LEFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPSQEAPEYVHMRTISPETAPDKATIIKIGFEKGDAVSINGE
RLSPATLLAKLNDYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDELMPRYAELIYY
GFWFSPEREMLQAAIDHSQHHVEGEVTLKLYKGNVMVIGRESAKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTL
AARDRK
>Mature_405_residues
SKWKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGIKEIFIEDVREEFVRDFVFPMF
RANAVYEGVYLLGTSIARPLISKHLIDIAKKTGADAIAHGATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTQLL
EFAEQHQIPVAKDKKGEAPFSVDANLLHSSSEGKVLEDPSQEAPEYVHMRTISPETAPDKATIIKIGFEKGDAVSINGER
LSPATLLAKLNDYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDELMPRYAELIYYG
FWFSPEREMLQAAIDHSQHHVEGEVTLKLYKGNVMVIGRESAKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLA
ARDRK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=398, Percent_Identity=49.2462311557789, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI16950633, Length=398, Percent_Identity=49.2462311557789, Blast_Score=374, Evalue=1e-104,
Organism=Escherichia coli, GI1789563, Length=409, Percent_Identity=28.1173594132029, Blast_Score=131, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=404, Percent_Identity=43.3168316831683, Blast_Score=330, Evalue=2e-91,
Organism=Drosophila melanogaster, GI21358151, Length=402, Percent_Identity=48.5074626865672, Blast_Score=358, Evalue=3e-99,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 45254; Mature: 45123

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKWKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGI
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHH
KEIFIEDVREEFVRDFVFPMFRANAVYEGVYLLGTSIARPLISKHLIDIAKKTGADAIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTQLLEFAEQHQIPVAKDKKGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPSQEAPEYVHMRTISPETAPDKATIIKIGFEKGDAVSINGE
CCCCHHHEECCCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCC
RLSPATLLAKLNDYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLD
CCCHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHHC
RGAAHLKDELMPRYAELIYYGFWFSPEREMLQAAIDHSQHHVEGEVTLKLYKGNVMVIGR
CCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHCCCHHCCCCEEEEEEEECCEEEEEC
ESAKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLAARDRK
HHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCCC
>Mature Secondary Structure 
SKWKDVKKVVLAYSGGLDTSIILKWLQTELGAEVVTFTADLGQGEELEPARKKAEMLGI
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHH
KEIFIEDVREEFVRDFVFPMFRANAVYEGVYLLGTSIARPLISKHLIDIAKKTGADAIAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHC
GATGKGNDQVRFELSAYALNPDIKIIAPWRDWSFKSRTQLLEFAEQHQIPVAKDKKGEAP
CCCCCCCCEEEEEEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
FSVDANLLHSSSEGKVLEDPSQEAPEYVHMRTISPETAPDKATIIKIGFEKGDAVSINGE
CCCCHHHEECCCCCCEECCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEECCC
RLSPATLLAKLNDYGRDNGIGRLDLVENRFVGMKSRGVYETPGGTILLAAHRAIESITLD
CCCHHHHHHHHHHCCCCCCCEEEEEHHCCCCCCCCCCCEECCCCEEEEEEHHHHHHHHHC
RGAAHLKDELMPRYAELIYYGFWFSPEREMLQAAIDHSQHHVEGEVTLKLYKGNVMVIGR
CCHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHCCCHHCCCCEEEEEEEECCEEEEEC
ESAKSLYSDKLVTFEDDQGAYDQKDAAGFIKLNALRLRTLAARDRK
HHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA