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The map label for this gene is trpS

Identifier: 161486701

GI number: 161486701

Start: 154838

End: 155905

Strand: Reverse

Name: trpS

Synonym: BR0142

Alternate gene names: 161486701

Gene position: 155905-154838 (Counterclockwise)

Preceding gene: 23501055

Following gene: 23501053

Centisome position: 7.4

GC content: 59.64

Gene sequence:

>1068_bases
ATGAGCACGTTCAAACCGCTTGTCTTCTCCGGCGTCCAACCGACCGGAAATCTTCACCTCGGCAATTATCTCGGGGCGAT
CAAGCGGTGGGTCGAAGTCCAGAAGACCGAGGAATGCATCTATTGCGTCGTGGACATGCACGCGCTGACCGTTTCGCCCG
ATCCGGTTGAGTTGATGCAGTCGACCCGCGAAGTCACCGCCGCTTTTCTCGCCGCCGGCATCGACCCGAAGAAGAGCATC
GTCTTCAACCAGAGCCGCGTCATGCAGCATGCGGAGCTTGCCTGGGTGTTCAACTGCGTCGCGCGCATTGGCTGGATGAG
CCGCATGACCCAGTTCAAGGACAAGGCAGGCAAGGATCGCGAGAATGCCTCGCTCGGTCTTTTTGCCTATCCGAGCCTGA
TGGCCGCCGATATTCTGCTTTATCGCGCAACCGCAGTGCCGGTTGGCGAGGACCAGAAGCAGCATCTGGAGCTGACCCGC
GATATTGCGCAGAAGTTCAACAACGACTATTCCGACCGCATCGCCTCGCTTGGTGTGGGCGTCGATATGAAAGTGGGCGA
CGAGCAGGTTTCAGGCTTCTTCCCGCTGACGGAACCGATGATCTCTGGCCCCGCCATGCGCATCATGTCGCTGCGCGACG
GCACCAAGAAAATGTCGAAGTCCGATCCGTCCGACCTGTCACGCATCAACCTGATCGACGATGAGGACACGATCACCAAG
AAAATCCGCAAGGCCAAGACCGATTCCGACGGCCTGCCCTCAGAAGTGGACGGGCTGGAAGGCCGCCCGGAGGCCGATAA
TCTCGTCGGCATTTACGCCGCCCTCTCCTCGACCACCAAGGAAGATGTGCTGAAGGAATTCGGCGGACGCCAGTTCTCCG
ACCTGAAGGCCTCGCTTGCCGATCTCGCCGTTGCCAGGCTTTCGCCGATCACGCATGAAATGCGTCGCCTGGTGGCCGAT
CCGGCCCATATCGACAGCGTTCTGCGTGATGGCGGTGAGCAGGCAGGCGCGATTGCCGAACAGACCATGCGTCATGTCCG
CGATATTGTCGGCTGGTTGCAGAACTGA

Upstream 100 bases:

>100_bases
ATAATTAAGCTTGAAAGCCTGCCATGCGTCTGCGAAAACACGCCGCATCATTATCACCCTTCGCTCTCCACCGGCGCAGG
TCAACCTTCAGGAACCTATC

Downstream 100 bases:

>100_bases
TTAAAAATGGAAGGCTGCCCGCTTGCGGGCAGCTTTCCGCGATGGCACATTGCCGCCATGGTATCCAGACGACTTAGCCG
CGAAGCCGGCCACCGACGCA

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSTFKPLVFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALTVSPDPVELMQSTREVTAAFLAAGIDPKKSI
VFNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGKDRENASLGLFAYPSLMAADILLYRATAVPVGEDQKQHLELTR
DIAQKFNNDYSDRIASLGVGVDMKVGDEQVSGFFPLTEPMISGPAMRIMSLRDGTKKMSKSDPSDLSRINLIDDEDTITK
KIRKAKTDSDGLPSEVDGLEGRPEADNLVGIYAALSSTTKEDVLKEFGGRQFSDLKASLADLAVARLSPITHEMRRLVAD
PAHIDSVLRDGGEQAGAIAEQTMRHVRDIVGWLQN

Sequences:

>Translated_355_residues
MSTFKPLVFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALTVSPDPVELMQSTREVTAAFLAAGIDPKKSI
VFNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGKDRENASLGLFAYPSLMAADILLYRATAVPVGEDQKQHLELTR
DIAQKFNNDYSDRIASLGVGVDMKVGDEQVSGFFPLTEPMISGPAMRIMSLRDGTKKMSKSDPSDLSRINLIDDEDTITK
KIRKAKTDSDGLPSEVDGLEGRPEADNLVGIYAALSSTTKEDVLKEFGGRQFSDLKASLADLAVARLSPITHEMRRLVAD
PAHIDSVLRDGGEQAGAIAEQTMRHVRDIVGWLQN
>Mature_354_residues
STFKPLVFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALTVSPDPVELMQSTREVTAAFLAAGIDPKKSIV
FNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGKDRENASLGLFAYPSLMAADILLYRATAVPVGEDQKQHLELTRD
IAQKFNNDYSDRIASLGVGVDMKVGDEQVSGFFPLTEPMISGPAMRIMSLRDGTKKMSKSDPSDLSRINLIDDEDTITKK
IRKAKTDSDGLPSEVDGLEGRPEADNLVGIYAALSSTTKEDVLKEFGGRQFSDLKASLADLAVARLSPITHEMRRLVADP
AHIDSVLRDGGEQAGAIAEQTMRHVRDIVGWLQN

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=351, Percent_Identity=39.8860398860399, Blast_Score=271, Evalue=5e-73,
Organism=Homo sapiens, GI41352700, Length=199, Percent_Identity=45.7286432160804, Blast_Score=192, Evalue=3e-49,
Organism=Escherichia coli, GI1789786, Length=349, Percent_Identity=42.9799426934097, Blast_Score=300, Evalue=8e-83,
Organism=Caenorhabditis elegans, GI71982800, Length=358, Percent_Identity=35.195530726257, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71982793, Length=358, Percent_Identity=35.195530726257, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320474, Length=364, Percent_Identity=37.0879120879121, Blast_Score=222, Evalue=6e-59,
Organism=Drosophila melanogaster, GI24666151, Length=347, Percent_Identity=36.3112391930836, Blast_Score=221, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_BRUME (P67586)

Other databases:

- EMBL:   AE008917
- PIR:   AH3477
- RefSeq:   NP_540723.2
- ProteinModelPortal:   P67586
- SMR:   P67586
- GeneID:   1197517
- GenomeReviews:   AE008917_GR
- KEGG:   bme:BMEI1806
- NMPDR:   fig|224914.1.peg.1805
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- PhylomeDB:   P67586
- ProtClustDB:   PRK00927
- BioCyc:   BMEL224914:BMEI1806-MONOMER
- BRENDA:   6.1.1.2
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 39130; Mature: 38999

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKPLVFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALTVSPDPVELMQ
CCCCCCHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEEHEEEECCCCHHHHHH
STREVTAAFLAAGIDPKKSIVFNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGKDR
HHHHHHHHHHHHCCCCHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENASLGLFAYPSLMAADILLYRATAVPVGEDQKQHLELTRDIAQKFNNDYSDRIASLGVG
CCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
VDMKVGDEQVSGFFPLTEPMISGPAMRIMSLRDGTKKMSKSDPSDLSRINLIDDEDTITK
CEECCCHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHEECCCCHHHHHH
KIRKAKTDSDGLPSEVDGLEGRPEADNLVGIYAALSSTTKEDVLKEFGGRQFSDLKASLA
HHHHHCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHH
DLAVARLSPITHEMRRLVADPAHIDSVLRDGGEQAGAIAEQTMRHVRDIVGWLQN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STFKPLVFSGVQPTGNLHLGNYLGAIKRWVEVQKTEECIYCVVDMHALTVSPDPVELMQ
CCCCCHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHEEEHEEEECCCCHHHHHH
STREVTAAFLAAGIDPKKSIVFNQSRVMQHAELAWVFNCVARIGWMSRMTQFKDKAGKDR
HHHHHHHHHHHHCCCCHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ENASLGLFAYPSLMAADILLYRATAVPVGEDQKQHLELTRDIAQKFNNDYSDRIASLGVG
CCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
VDMKVGDEQVSGFFPLTEPMISGPAMRIMSLRDGTKKMSKSDPSDLSRINLIDDEDTITK
CEECCCHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHEECCCCHHHHHH
KIRKAKTDSDGLPSEVDGLEGRPEADNLVGIYAALSSTTKEDVLKEFGGRQFSDLKASLA
HHHHHCCCCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHH
DLAVARLSPITHEMRRLVADPAHIDSVLRDGGEQAGAIAEQTMRHVRDIVGWLQN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688