Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 161486694

GI number: 161486694

Start: 1515905

End: 1517572

Strand: Reverse

Name: leuA [H]

Synonym: BR1566

Alternate gene names: 161486694

Gene position: 1517572-1515905 (Counterclockwise)

Preceding gene: 23502431

Following gene: 23502429

Centisome position: 72.0

GC content: 57.37

Gene sequence:

>1668_bases
ATGCCTATTGCCGGTACCAAATACGCGCCGTTCCCTGCGCCGCAGCTCGATGACCGCACTTGGCCTTCCAAACGCATCGA
AAAGGCGCCGATCTGGTGTTCGGTCGATCTGCGCGACGGCAACCAGGCTTTGATCGACCCCATGGGGCATGACCGCAAGG
AGCGCATGTTCCGCCTGCTGATCGATATGGGTTTCCCGGAAATCGAGATCGGTTTCCCGTCCGCTTCGCAGACGGATTTC
GACTTCTGCCGCTGGGCCATTGAACAGGGCGATGTTCCTGACGACGTGGATTTGCAGGTTCTGGTCCAGTGCCGTCCTGA
ATTGATTACGCGCACTTTCGAGGCGCTGGAAGGCGCGAAGACGCCGATCATCCATTTCTACAATTCCACCAGCGAATTGC
AGCGCCGCGTGGTGTTTGCCAAGGATGTCGGCGGCATCAAGCAGATTGCAACCGATGCGGCCAAGATGATCATGGATATG
GCTGCCAAGGCTGGCGGCGGTTATCGCTTCCAGTATTCGCCGGAAAGCTTTACCGGCACCGAACTGGATGTGGCGCTGGA
AATCTGCAATGCGGTGATCGAGATCGTGAAGCCGACGCCGGACAACAAGCTGATCGTCAACCTGCCTTCGACGGTGGAGA
TGAACACGCCGAATGTCTATGCCGACCAGATCGAATGGATGTGCCGCAATCTGGACAATCGCGAGAGCCTGATTATCTCG
CTGCATCCGCATAATGACCGCGGCACGGGCATTGCCGCCACTGAACTGGGCCTGATGGCAGGCGCTGATCGCGTGGAAGG
CACGCTGTTCGGCAATGGCGAGCGCACGGGCAATGTGGATGTGGTGACGCTGGCGCTCAACATGTACACGCAGGGCATCG
ACCCCGGGCTGGATTGCACCGATATCAACCGGATGAAGGAAGTCTACGAATATTCCAACCAGTTGAAGATCGCCGAGCGC
CATCCTTATGTGGGCGAGCTGGTCTATACGGCTTTCTCCGGCTCGCATCAGGATGCGATCAACAAGGGCATGAAAGCGCG
CCGTTCGGCCAATTCGCCGGTTTGGGAAGTTCCTTACCTGCCGATCGATCCGCAGGATGTGGGCCGCTCCTACGAGGCGA
TCATCCGCATCAATTCGCAATCGGGCAAGGGCGGTATCGCCTATATCCTGCAAGCCGATTACGGCCTGAACCTGCCGCGC
AACCTGCAGGTCGAGTTCCGCGAAATCATCCAGCACATTACGGATGAAGAGGGCAAGGAGCTGCCATCGAAGCGCATTTA
CGAGGAGTTCCAGAAACTCTATGTGACGCAGCCCGATGCCCGCATCAAGTTCGTGGATCACCACACCTATCCCGATCCTG
AGCAAAAGGGCCGGCGCATCCTGACGGCTGAGATCACCGATAACGGTGTCACCAAGACTATCGAGGGCAAGGGCACGGGA
CCGATCGATGGTTTCGTGGATGCGCTGTCGAAATATCTTGGCGTGAAAATGTCGGTGGTCGATTATTCCGAACATTCGCT
CCAGCAGGGTTCGGATGCTTCGGCCATTTCCTATGTCGAGATGGTTTATCCCGGCGGCAAGCTGTTCGGTGTCGGCATCA
ACGATAATATCGTCAGCGCCTCGCTTGAAGCCGTGGTGTCGGCGGCAAATCGTGTGATTGCGAAGTAG

Upstream 100 bases:

>100_bases
ACGGTATGGAACGCCGTTGAAAGCCGGGAGGAAACGAGAAATGAACCAGCCTGTCTCCACGCCAAAGTCCGTGTCCCAAG
CCGTATCGGCACGCAAGGGC

Downstream 100 bases:

>100_bases
AGCTACAGTTGCAAATTCGTCAGGCCGTGGCGAAAAGAGTGATTTTCGAGTACCGGAGCGCAGCGTATTTAAGGCTACGT
GAGCACCGGAACGCAGATAG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 555; Mature: 554

Protein sequence:

>555_residues
MPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLLIDMGFPEIEIGFPSASQTDF
DFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDM
AAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVYADQIEWMCRNLDNRESLIIS
LHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCTDINRMKEVYEYSNQLKIAER
HPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPR
NLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPDPEQKGRRILTAEITDNGVTKTIEGKGTG
PIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSASLEAVVSAANRVIAK

Sequences:

>Translated_555_residues
MPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLLIDMGFPEIEIGFPSASQTDF
DFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDM
AAKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVYADQIEWMCRNLDNRESLIIS
LHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCTDINRMKEVYEYSNQLKIAER
HPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPR
NLQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPDPEQKGRRILTAEITDNGVTKTIEGKGTG
PIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSASLEAVVSAANRVIAK
>Mature_554_residues
PIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLLIDMGFPEIEIGFPSASQTDFD
FCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAKTPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDMA
AKAGGGYRFQYSPESFTGTELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVYADQIEWMCRNLDNRESLIISL
HPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCTDINRMKEVYEYSNQLKIAERH
PYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYLPIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPRN
LQVEFREIIQHITDEEGKELPSKRIYEEFQKLYVTQPDARIKFVDHHTYPDPEQKGRRILTAEITDNGVTKTIEGKGTGP
IDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSASLEAVVSAANRVIAK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=548, Percent_Identity=23.3576642335766, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6324225, Length=563, Percent_Identity=46.7140319715808, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6324682, Length=565, Percent_Identity=47.0796460176991, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61635; Mature: 61503

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLL
CCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHH
IDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAK
HHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHECCCHHHHHHHHHHHCCCC
TPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDMAAKAGGGYRFQYSPESFTGT
CCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVYADQIEWMCRNLDNRESLIIS
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
LHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCT
EECCCCCCCCEEHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEHHHCCCCCCCCCH
DINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYL
HHHHHHHHHHHHCCEEEECCCCCHHHEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCC
PIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPRNLQVEFREIIQHITDEEGKE
CCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHC
LPSKRIYEEFQKLYVTQPDARIKFVDHHTYPDPEQKGRRILTAEITDNGVTKTIEGKGTG
CCHHHHHHHHHHHEECCCCCEEEEEECCCCCCHHHHCCEEEEEEECCCCCEEEECCCCCC
PIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSA
CHHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCCHHEEEEEEECCCEEEEECCCCCHHHH
SLEAVVSAANRVIAK
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
PIAGTKYAPFPAPQLDDRTWPSKRIEKAPIWCSVDLRDGNQALIDPMGHDRKERMFRLL
CCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHH
IDMGFPEIEIGFPSASQTDFDFCRWAIEQGDVPDDVDLQVLVQCRPELITRTFEALEGAK
HHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHECCCHHHHHHHHHHHCCCC
TPIIHFYNSTSELQRRVVFAKDVGGIKQIATDAAKMIMDMAAKAGGGYRFQYSPESFTGT
CCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ELDVALEICNAVIEIVKPTPDNKLIVNLPSTVEMNTPNVYADQIEWMCRNLDNRESLIIS
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEECCCCCHHHHHHHHHHCCCCCCEEEEE
LHPHNDRGTGIAATELGLMAGADRVEGTLFGNGERTGNVDVVTLALNMYTQGIDPGLDCT
EECCCCCCCCEEHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEHHHCCCCCCCCCH
DINRMKEVYEYSNQLKIAERHPYVGELVYTAFSGSHQDAINKGMKARRSANSPVWEVPYL
HHHHHHHHHHHHCCEEEECCCCCHHHEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCC
PIDPQDVGRSYEAIIRINSQSGKGGIAYILQADYGLNLPRNLQVEFREIIQHITDEEGKE
CCCHHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHC
LPSKRIYEEFQKLYVTQPDARIKFVDHHTYPDPEQKGRRILTAEITDNGVTKTIEGKGTG
CCHHHHHHHHHHHEECCCCCEEEEEECCCCCCHHHHCCEEEEEEECCCCCEEEECCCCCC
PIDGFVDALSKYLGVKMSVVDYSEHSLQQGSDASAISYVEMVYPGGKLFGVGINDNIVSA
CHHHHHHHHHHHHCCEEEEEECCHHHHCCCCCCCHHEEEEEEECCCEEEEECCCCCHHHH
SLEAVVSAANRVIAK
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]