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The map label for this gene is splB [H]

Identifier: 28563934

GI number: 28563934

Start: 2326324

End: 2327352

Strand: Reverse

Name: splB [H]

Synonym: OB2284

Alternate gene names: 28563934

Gene position: 2327352-2326324 (Counterclockwise)

Preceding gene: 28563935

Following gene: 23099738

Centisome position: 64.11

GC content: 34.79

Gene sequence:

>1029_bases
ATGGTTAGACCCTTTGTCCCTCAATTAATATATGTAGAACCAAAAGCAATGGAGTATAGCTTGGGTAAATCACTAGTAAC
GAAGTTTGAAAATATGGGAATTGAAATTAGAGAAACAACTTCTCATAATCAAATTCGAAATTTGCCAGGAGACAATGATT
TTCAAAAGTATCGTAACGCAAAATCTACTTTAGTTATCGGTGTAAGAAAAACGTTGAAGTTTGATACATCAAAACCTTCT
GCTGAATATGCAATTCCTTTTGCTACTGGTTGCATGGGACATTGTCATTATTGTTATTTACAAACAACTATGGGGAGTAA
GCCCTATATTCGTACGTATGTAAATATTGATGAAATTTTTGATGCTGCAGAAGGGTATATGCAAGAAAGAGCTCCATTGG
ATACAAGATTTGAAGCATCATGTACTTCCGATATTGTAGGTGTTGATCATCTTACGCATACGTTAAAACATGCCATCGAG
TATTTTGGGAAAAGCGAACACGGTAAACTTCGATTTGTTACAAAGTTTGCACATGTAGATCATTTACTTGATGCGGATCA
TAAAGGGAGAACAAGATTTCGTTTTAGTATTAATAATAACCATATTATTAAATACTTTGAGCCTGGTACGTCTCGATTAA
ATGAAAGGATAGAAGCTGCTGTTAAGGTTGCTGAAGCGGGATATCCTTTAGGTTTTATTATTGCTCCTATCTACTTGCAT
GATGGTTGGAAAGAAGGGTATAAAGAAATGTTTGAAAAACTCGATGAAGCTCTTCCACCATTTGCAAGGAAAGATTTAAC
CTTTGAAATGATTCAACATCGATTCACAAAGCCTGCAAAACGTGTAATCGAGAAAAATTATCCGATGACTAAATTGGAGT
TAGATGAATCAAAAAGAAAAACCAAATGGGGCAGATATGGTATTTATAAATATGTGTACCAAGATCAAGAACAACAGGAT
ATAAAAAACACATTGGGTGGTTGGATAAACGACTATTTCCCAGCTAGCACTATCGAGTATTTCACGTAA

Upstream 100 bases:

>100_bases
TATTACCCGCTTACAGATGAATATGCAATATTTACTTCAGAAGAAGCAGCAGAGAATGCTTTTAGAGAAGCATTTTTATC
TGATGATGAGGGACAATTTT

Downstream 100 bases:

>100_bases
TGGCTCGTACTTGCCACACCTCCTTAAATTTTCTTATATTGGTATGCTCTTTTTCAAGAGGATAATCGTTAAGAAGTATG
AAATTATAAAAACAACAAAT

Product: spore photoproduct lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MVRPFVPQLIYVEPKAMEYSLGKSLVTKFENMGIEIRETTSHNQIRNLPGDNDFQKYRNAKSTLVIGVRKTLKFDTSKPS
AEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNIDEIFDAAEGYMQERAPLDTRFEASCTSDIVGVDHLTHTLKHAIE
YFGKSEHGKLRFVTKFAHVDHLLDADHKGRTRFRFSINNNHIIKYFEPGTSRLNERIEAAVKVAEAGYPLGFIIAPIYLH
DGWKEGYKEMFEKLDEALPPFARKDLTFEMIQHRFTKPAKRVIEKNYPMTKLELDESKRKTKWGRYGIYKYVYQDQEQQD
IKNTLGGWINDYFPASTIEYFT

Sequences:

>Translated_342_residues
MVRPFVPQLIYVEPKAMEYSLGKSLVTKFENMGIEIRETTSHNQIRNLPGDNDFQKYRNAKSTLVIGVRKTLKFDTSKPS
AEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNIDEIFDAAEGYMQERAPLDTRFEASCTSDIVGVDHLTHTLKHAIE
YFGKSEHGKLRFVTKFAHVDHLLDADHKGRTRFRFSINNNHIIKYFEPGTSRLNERIEAAVKVAEAGYPLGFIIAPIYLH
DGWKEGYKEMFEKLDEALPPFARKDLTFEMIQHRFTKPAKRVIEKNYPMTKLELDESKRKTKWGRYGIYKYVYQDQEQQD
IKNTLGGWINDYFPASTIEYFT
>Mature_342_residues
MVRPFVPQLIYVEPKAMEYSLGKSLVTKFENMGIEIRETTSHNQIRNLPGDNDFQKYRNAKSTLVIGVRKTLKFDTSKPS
AEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNIDEIFDAAEGYMQERAPLDTRFEASCTSDIVGVDHLTHTLKHAIE
YFGKSEHGKLRFVTKFAHVDHLLDADHKGRTRFRFSINNNHIIKYFEPGTSRLNERIEAAVKVAEAGYPLGFIIAPIYLH
DGWKEGYKEMFEKLDEALPPFARKDLTFEMIQHRFTKPAKRVIEKNYPMTKLELDESKRKTKWGRYGIYKYVYQDQEQQD
IKNTLGGWINDYFPASTIEYFT

Specific function: Involved in repair of UV radiation-induced DNA damage during spore germination. Can repair thymine dimer 5-thyminyl-5,6- dihydrothymine (known as spore photoproduct (SP)) by in situ monomerization of SP to two thymines [H]

COG id: COG1533

COG function: function code L; DNA repair photolyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. SPL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR004594 [H]

Pfam domain/function: NA

EC number: =4.1.99.14 [H]

Molecular weight: Translated: 39784; Mature: 39784

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRPFVPQLIYVEPKAMEYSLGKSLVTKFENMGIEIRETTSHNQIRNLPGDNDFQKYRNA
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCCCCCHHHHHCCC
KSTLVIGVRKTLKFDTSKPSAEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNIDEIF
CCEEEEEEHHHEEECCCCCCCCEECCHHHCCCCCCEEEEEEECCCCCCCEEEEECHHHHH
DAAEGYMQERAPLDTRFEASCTSDIVGVDHLTHTLKHAIEYFGKSEHGKLRFVTKFAHVD
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHH
HLLDADHKGRTRFRFSINNNHIIKYFEPGTSRLNERIEAAVKVAEAGYPLGFIIAPIYLH
HHHCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHEEHHHHC
DGWKEGYKEMFEKLDEALPPFARKDLTFEMIQHRFTKPAKRVIEKNYPMTKLELDESKRK
CCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCHHHHHHHHCCCCCEEEECCHHHHH
TKWGRYGIYKYVYQDQEQQDIKNTLGGWINDYFPASTIEYFT
HCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHCCCC
>Mature Secondary Structure
MVRPFVPQLIYVEPKAMEYSLGKSLVTKFENMGIEIRETTSHNQIRNLPGDNDFQKYRNA
CCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHCCCCCCHHHHHCCC
KSTLVIGVRKTLKFDTSKPSAEYAIPFATGCMGHCHYCYLQTTMGSKPYIRTYVNIDEIF
CCEEEEEEHHHEEECCCCCCCCEECCHHHCCCCCCEEEEEEECCCCCCCEEEEECHHHHH
DAAEGYMQERAPLDTRFEASCTSDIVGVDHLTHTLKHAIEYFGKSEHGKLRFVTKFAHVD
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHH
HLLDADHKGRTRFRFSINNNHIIKYFEPGTSRLNERIEAAVKVAEAGYPLGFIIAPIYLH
HHHCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHEEHHHHC
DGWKEGYKEMFEKLDEALPPFARKDLTFEMIQHRFTKPAKRVIEKNYPMTKLELDESKRK
CCHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCHHHHHHHHCCCCCEEEECCHHHHH
TKWGRYGIYKYVYQDQEQQDIKNTLGGWINDYFPASTIEYFT
HCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8449881; 9384377; 9353924 [H]