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The map label for this gene is vsr [H]

Identifier: 23100796

GI number: 23100796

Start: 3481295

End: 3481717

Strand: Direct

Name: vsr [H]

Synonym: OB3341

Alternate gene names: 23100796

Gene position: 3481295-3481717 (Clockwise)

Preceding gene: 23100787

Following gene: 23100803

Centisome position: 95.89

GC content: 29.31

Gene sequence:

>423_bases
ATGAGCGATATAATGACAAAACAACAAAGAAGCAATAATATGAAAGCAATAAAATCTGTTTCAAAATTGGAATCTATAGT
TTCAAAAAAACTTTGGGCAAGAGGCTTTCGCTTTAGGAGAAACACACAAAACTTATTCGGAAAACCTGATATCTCAATAA
AAAAATATAAGATAGTTATATTTATTGACTCTTGTTTTTGGCACCAATGTCCTTTGCATGCTAATATGCCAAAAAGCCAT
ACAGACTATTGGGAAAAAAAACTTGAGCGTAATAAAGAGCGTGATCAAGAAGTTAATAATTATTATAGTGACAAAGGATG
GTATTTAAAAAGAATTTGGGAGCATGAAATTAAAAATGACATAGATAAAGTTATTCAAGATACTGCAGAATTTATAACTC
TAGCTCAAACAGAATTTAGATAA

Upstream 100 bases:

>100_bases
ATACTAATATTTTTAATTGTATTCAAAACAAATTAGAAAAAAAAGGAAAAAGTTTTAAATTATACTTTAACAGGAAAAGA
AAAAATAAAGGAGTTATAAC

Downstream 100 bases:

>100_bases
TACCAGCCTGTTTGTCAGTTTTATTAATAACTATTCCTTTATTACTATTTCCTATTTATTTAAGTAGTTATTTAATTTTT
TTGCTATTTTAATATCGACA

Product: very-short-patch-repair endonuclease

Products: DNA [C]

Alternate protein names: V.NaeI [H]

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MSDIMTKQQRSNNMKAIKSVSKLESIVSKKLWARGFRFRRNTQNLFGKPDISIKKYKIVIFIDSCFWHQCPLHANMPKSH
TDYWEKKLERNKERDQEVNNYYSDKGWYLKRIWEHEIKNDIDKVIQDTAEFITLAQTEFR

Sequences:

>Translated_140_residues
MSDIMTKQQRSNNMKAIKSVSKLESIVSKKLWARGFRFRRNTQNLFGKPDISIKKYKIVIFIDSCFWHQCPLHANMPKSH
TDYWEKKLERNKERDQEVNNYYSDKGWYLKRIWEHEIKNDIDKVIQDTAEFITLAQTEFR
>Mature_139_residues
SDIMTKQQRSNNMKAIKSVSKLESIVSKKLWARGFRFRRNTQNLFGKPDISIKKYKIVIFIDSCFWHQCPLHANMPKSHT
DYWEKKLERNKERDQEVNNYYSDKGWYLKRIWEHEIKNDIDKVIQDTAEFITLAQTEFR

Specific function: May nick NaeI sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=122, Percent_Identity=32.7868852459016, Blast_Score=80, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 16964; Mature: 16832

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIMTKQQRSNNMKAIKSVSKLESIVSKKLWARGFRFRRNTQNLFGKPDISIKKYKIVI
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEE
FIDSCFWHQCPLHANMPKSHTDYWEKKLERNKERDQEVNNYYSDKGWYLKRIWEHEIKND
EEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IDKVIQDTAEFITLAQTEFR
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDIMTKQQRSNNMKAIKSVSKLESIVSKKLWARGFRFRRNTQNLFGKPDISIKKYKIVI
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCEEEEEEEE
FIDSCFWHQCPLHANMPKSHTDYWEKKLERNKERDQEVNNYYSDKGWYLKRIWEHEIKND
EEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IDKVIQDTAEFITLAQTEFR
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7698663 [H]