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The map label for this gene is yqiG [H]

Identifier: 23100770

GI number: 23100770

Start: 3455050

End: 3456171

Strand: Direct

Name: yqiG [H]

Synonym: OB3315

Alternate gene names: 23100770

Gene position: 3455050-3456171 (Clockwise)

Preceding gene: 23100768

Following gene: 23100771

Centisome position: 95.17

GC content: 43.23

Gene sequence:

>1122_bases
ATGAATGCAAATAATCACGATATGTTTTCTCCATTAACATTTAAAAATGGACTTGAATTAAAGAACCGATTGGTAATGGC
TCCGATGACACACTACTCATCCAATCCAGACGGGACAGTAAGTGATGAGGAGATAAGCTATTATGCTCGCCGTTCTAAAG
GCGTAGGCATGGTCATTACCGCAACAACTTATGTCACACCGAATGGAAAAGGATTCCCTAACCAATTTTCGGCAGTAGAA
GATGGCATGATTCCGAGCTTAAGCAAATTGGCAAAAGGAATTCAGAATGAAGGCGCCAAGGCCATTTTACAAATTTTCCA
TGGTGGAAAAATGTGCCCTCCTTCTCTTATTCCAGATGGAGATGTCGTAAGCGCTAGTAACATTCCAGCAAATAGCGAAG
CTGCCGAGCCACGGCCTTTGAAGGAATCAGAAATCGAGGAAATTATTGAGGCGTTTGGCGAAACGACACGCAGAGCCATT
GAAGCAGGCTATGATGGCGTTGAAATTCACGGAGCGAACGGCTACTTACTTCAGCAATTCTTTTCTCCATTTTCCAACCG
TCGCGACGATCGATTTGGAGGCAGTTTGGAAAAACGGATGGCTTTCCCGCTTGCTGTAATCGAAAAAGTAAAAAGCGTTG
TCGCAGAACATGCGGATGCGTCCTTCATCGTTGGTTACCGCTTTTCACCAGAAGAGCCAGAGACACCAGGAATTACGATG
GATGAAACCCTTCAATTCGTAGACGTTTTAGCGGATAAACAGTTAGATTATCTACATGTTTCCTTAATGGACTTTTTCTC
CAAAGCCCGAAGAGGTGTAGAAGACCTAAATAAAACGAGGATCAGCTACTTACTGGAAACGATCGATAATCGCGTACCGT
TGATTGGTGTTGGTTCTATTTATACAGCAGAAGATGCCCGAAAAGCATTTAATACGGGGATCCCCCTACTTGCAATAGGA
AGAGAAATTATCATCGATCCTGATTGGGTAGAGAAAGTTGCCGAAGGAAGAGATGATGAAATTATCACCGAAATTAATAA
AGACAAACAGGAAGAATTAGTCGTCCCTGATCCACTTTGGAACGTTATTACCCATGCACCTGGATGGTTTCCTGGAGTAT
AG

Upstream 100 bases:

>100_bases
TAACACTTATTAGAGGGTAGAGGCGATTCGCACAACCAAGCAAAATTCTATACAAATCCCCCTCCATTTACTACTATATC
TTAACAAGGAGGCGTACAGC

Downstream 100 bases:

>100_bases
ACCGAGAATGATAAAGTCCGTTCTCTACATTATTGTAGGGGATGGCTTTTTTTAGTTTTTTCATTACATTAAAGAAAACA
ACTACTCCAACGATTTGTTT

Product: NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNANNHDMFSPLTFKNGLELKNRLVMAPMTHYSSNPDGTVSDEEISYYARRSKGVGMVITATTYVTPNGKGFPNQFSAVE
DGMIPSLSKLAKGIQNEGAKAILQIFHGGKMCPPSLIPDGDVVSASNIPANSEAAEPRPLKESEIEEIIEAFGETTRRAI
EAGYDGVEIHGANGYLLQQFFSPFSNRRDDRFGGSLEKRMAFPLAVIEKVKSVVAEHADASFIVGYRFSPEEPETPGITM
DETLQFVDVLADKQLDYLHVSLMDFFSKARRGVEDLNKTRISYLLETIDNRVPLIGVGSIYTAEDARKAFNTGIPLLAIG
REIIIDPDWVEKVAEGRDDEIITEINKDKQEELVVPDPLWNVITHAPGWFPGV

Sequences:

>Translated_373_residues
MNANNHDMFSPLTFKNGLELKNRLVMAPMTHYSSNPDGTVSDEEISYYARRSKGVGMVITATTYVTPNGKGFPNQFSAVE
DGMIPSLSKLAKGIQNEGAKAILQIFHGGKMCPPSLIPDGDVVSASNIPANSEAAEPRPLKESEIEEIIEAFGETTRRAI
EAGYDGVEIHGANGYLLQQFFSPFSNRRDDRFGGSLEKRMAFPLAVIEKVKSVVAEHADASFIVGYRFSPEEPETPGITM
DETLQFVDVLADKQLDYLHVSLMDFFSKARRGVEDLNKTRISYLLETIDNRVPLIGVGSIYTAEDARKAFNTGIPLLAIG
REIIIDPDWVEKVAEGRDDEIITEINKDKQEELVVPDPLWNVITHAPGWFPGV
>Mature_373_residues
MNANNHDMFSPLTFKNGLELKNRLVMAPMTHYSSNPDGTVSDEEISYYARRSKGVGMVITATTYVTPNGKGFPNQFSAVE
DGMIPSLSKLAKGIQNEGAKAILQIFHGGKMCPPSLIPDGDVVSASNIPANSEAAEPRPLKESEIEEIIEAFGETTRRAI
EAGYDGVEIHGANGYLLQQFFSPFSNRRDDRFGGSLEKRMAFPLAVIEKVKSVVAEHADASFIVGYRFSPEEPETPGITM
DETLQFVDVLADKQLDYLHVSLMDFFSKARRGVEDLNKTRISYLLETIDNRVPLIGVGSIYTAEDARKAFNTGIPLLAIG
REIIIDPDWVEKVAEGRDDEIITEINKDKQEELVVPDPLWNVITHAPGWFPGV

Specific function: Unknown

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=355, Percent_Identity=28.7323943661972, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=26.8011527377522, Blast_Score=114, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17559804, Length=352, Percent_Identity=28.125, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17559802, Length=350, Percent_Identity=28.2857142857143, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17564188, Length=352, Percent_Identity=26.9886363636364, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17565138, Length=349, Percent_Identity=26.3610315186246, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI72001454, Length=367, Percent_Identity=26.158038147139, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17540738, Length=266, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17566914, Length=264, Percent_Identity=28.030303030303, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321973, Length=359, Percent_Identity=28.4122562674095, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6325086, Length=364, Percent_Identity=27.1978021978022, Blast_Score=106, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 41210; Mature: 41210

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNANNHDMFSPLTFKNGLELKNRLVMAPMTHYSSNPDGTVSDEEISYYARRSKGVGMVIT
CCCCCCCCCCCCCCCCCHHHHHCEEEEEHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
ATTYVTPNGKGFPNQFSAVEDGMIPSLSKLAKGIQNEGAKAILQIFHGGKMCPPSLIPDG
EEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
DVVSASNIPANSEAAEPRPLKESEIEEIIEAFGETTRRAIEAGYDGVEIHGANGYLLQQF
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHH
FSPFSNRRDDRFGGSLEKRMAFPLAVIEKVKSVVAEHADASFIVGYRFSPEEPETPGITM
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCH
DETLQFVDVLADKQLDYLHVSLMDFFSKARRGVEDLNKTRISYLLETIDNRVPLIGVGSI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCE
YTAEDARKAFNTGIPLLAIGREIIIDPDWVEKVAEGRDDEIITEINKDKQEELVVPDPLW
EEHHHHHHHHHCCCCCEECCCEEEECCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCHHH
NVITHAPGWFPGV
HHHHCCCCCCCCC
>Mature Secondary Structure
MNANNHDMFSPLTFKNGLELKNRLVMAPMTHYSSNPDGTVSDEEISYYARRSKGVGMVIT
CCCCCCCCCCCCCCCCCHHHHHCEEEEEHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
ATTYVTPNGKGFPNQFSAVEDGMIPSLSKLAKGIQNEGAKAILQIFHGGKMCPPSLIPDG
EEEEECCCCCCCCCHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
DVVSASNIPANSEAAEPRPLKESEIEEIIEAFGETTRRAIEAGYDGVEIHGANGYLLQQF
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHH
FSPFSNRRDDRFGGSLEKRMAFPLAVIEKVKSVVAEHADASFIVGYRFSPEEPETPGITM
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCH
DETLQFVDVLADKQLDYLHVSLMDFFSKARRGVEDLNKTRISYLLETIDNRVPLIGVGSI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCE
YTAEDARKAFNTGIPLLAIGREIIIDPDWVEKVAEGRDDEIITEINKDKQEELVVPDPLW
EEHHHHHHHHHCCCCCEECCCEEEECCHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCHHH
NVITHAPGWFPGV
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]