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The map label for this gene is ribE [H]

Identifier: 23100670

GI number: 23100670

Start: 3347396

End: 3348022

Strand: Reverse

Name: ribE [H]

Synonym: OB3215

Alternate gene names: 23100670

Gene position: 3348022-3347396 (Counterclockwise)

Preceding gene: 23100671

Following gene: 23100669

Centisome position: 92.22

GC content: 40.35

Gene sequence:

>627_bases
TTGTTTACTGGAGTTGTTCAAACAATTGGGCAAGTAACGAATGTAGAGAAACGACGCGAAACAATTCAACTTGTCATTCA
TACAACGGAAGCTCAATTGCTGCATTACAACGTTGGCGACAGTATGGCTGTTAATGGTGTGTGTCTGACTGCCACAAAAG
TAGGCGAGGATACATTTACGGCTGATGTTATGCCAGAAACATTTCGATCGACCAATCTTCAATATGTGCGTATTGGTTCA
TCCGTTAATTTGGAAAGGGCGATGCGTGCAACAGATCGGTTTGAAGGACATGTCGTTGCGGGGCACGTGGATTCTACCGT
TACCGTGAAACGTACATGGAAGGACCAAAATGCATATTACATGAGTTTTACTTTACCGAAACCATATGCGCAAGAAGTAA
TTGCAAAAGGTTCGATTACCTTGGATGGTACGAGTTTAACGGTTGTAGAAAAATCCAGTCATGCATTTACGATTAGTTTG
ATTCCTCATTCACAACAAGCGTCGATTTTATCCCAACGTCGAGTGGGTGATTATGTAAATATGGAGACAGATATGCTTGC
TAAATATATTCTTCAAGCAAATAAGCAAACCTTATCACAGTCCTTTTTACAAGAGAATGGATTTTAA

Upstream 100 bases:

>100_bases
TACGAATTTAGAAGTTGCTTACTCTCTAGATCACTATAATCCCCTTTTTAAAAGATCACATTCATTTCTGATATTAAATT
TCATAAAAGGAGCTGATGAT

Downstream 100 bases:

>100_bases
GGAGGGTAAATATGAACGAGCTAAGCAAGATAGAGCAAGCGATCGAAGATTTACGAAACGGAAAGTTGATTATTGTTGCG
GATGATGCGGACCGAGAAGC

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MFTGVVQTIGQVTNVEKRRETIQLVIHTTEAQLLHYNVGDSMAVNGVCLTATKVGEDTFTADVMPETFRSTNLQYVRIGS
SVNLERAMRATDRFEGHVVAGHVDSTVTVKRTWKDQNAYYMSFTLPKPYAQEVIAKGSITLDGTSLTVVEKSSHAFTISL
IPHSQQASILSQRRVGDYVNMETDMLAKYILQANKQTLSQSFLQENGF

Sequences:

>Translated_208_residues
MFTGVVQTIGQVTNVEKRRETIQLVIHTTEAQLLHYNVGDSMAVNGVCLTATKVGEDTFTADVMPETFRSTNLQYVRIGS
SVNLERAMRATDRFEGHVVAGHVDSTVTVKRTWKDQNAYYMSFTLPKPYAQEVIAKGSITLDGTSLTVVEKSSHAFTISL
IPHSQQASILSQRRVGDYVNMETDMLAKYILQANKQTLSQSFLQENGF
>Mature_208_residues
MFTGVVQTIGQVTNVEKRRETIQLVIHTTEAQLLHYNVGDSMAVNGVCLTATKVGEDTFTADVMPETFRSTNLQYVRIGS
SVNLERAMRATDRFEGHVVAGHVDSTVTVKRTWKDQNAYYMSFTLPKPYAQEVIAKGSITLDGTSLTVVEKSSHAFTISL
IPHSQQASILSQRRVGDYVNMETDMLAKYILQANKQTLSQSFLQENGF

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=200, Percent_Identity=30.5, Blast_Score=111, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6319733, Length=201, Percent_Identity=36.81592039801, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23158; Mature: 23158

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGVVQTIGQVTNVEKRRETIQLVIHTTEAQLLHYNVGDSMAVNGVCLTATKVGEDTFT
CCCHHHHHHHHHHCHHHHHCEEEEEEEECCEEEEEEECCCCEEECCEEEEEEECCCCCEE
ADVMPETFRSTNLQYVRIGSSVNLERAMRATDRFEGHVVAGHVDSTVTVKRTWKDQNAYY
ECCCCHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCEEE
MSFTLPKPYAQEVIAKGSITLDGTSLTVVEKSSHAFTISLIPHSQQASILSQRRVGDYVN
EEEECCCHHHHHHHHCCCEEECCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHCCCCCC
METDMLAKYILQANKQTLSQSFLQENGF
CCHHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MFTGVVQTIGQVTNVEKRRETIQLVIHTTEAQLLHYNVGDSMAVNGVCLTATKVGEDTFT
CCCHHHHHHHHHHCHHHHHCEEEEEEEECCEEEEEEECCCCEEECCEEEEEEECCCCCEE
ADVMPETFRSTNLQYVRIGSSVNLERAMRATDRFEGHVVAGHVDSTVTVKRTWKDQNAYY
ECCCCHHHHCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEECCCCEEEEEEEECCCCEEE
MSFTLPKPYAQEVIAKGSITLDGTSLTVVEKSSHAFTISLIPHSQQASILSQRRVGDYVN
EEEECCCHHHHHHHHCCCEEECCCEEEEEECCCCEEEEEEECCCHHHHHHHHHHCCCCCC
METDMLAKYILQANKQTLSQSFLQENGF
CCHHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2106516; 7934829; 9384377; 7473709 [H]