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The map label for this gene is cwlM [H]

Identifier: 23100596

GI number: 23100596

Start: 3273254

End: 3274054

Strand: Direct

Name: cwlM [H]

Synonym: OB3141

Alternate gene names: 23100596

Gene position: 3273254-3274054 (Clockwise)

Preceding gene: 23100595

Following gene: 23100598

Centisome position: 90.16

GC content: 40.7

Gene sequence:

>801_bases
ATGAAACTATATCTTGATCCCGGACATGGTGGTAGTGATCCTGGAGCTGTCGGCAATGGACTACAAGAAAAAACAATCAA
CCTAGATATCGCTATAAGAATACGTGATTTATTACTCTCCAATTACAATAATGTATCTGTAAAAATGAGCAGGACATCAG
ATAGTACAAAAAGCTTATCCCAACGGACGAACGAGGCTAACAATTGGAATGCAGACTATTTTCTTTCTATTCACTGTAAC
TCCTTTAATGGTTCAGCTTACGGCTACGAGGATTTCATCCACAATAGTCTATCGGATCAATCAACTACTGCTAGATACCA
AAACATTATCCATGAAGAGGTAAGTAAAGTTAATCAACTGCGAAACCGCGGTAAAAAGAAAGCAAATTTCCATGTTTTAC
GCGAATCCCATATGCCTGCGCTTCTCACAGAGAACGGTTTTATTGACCATAGCGGTGATGCAGCAAAATTACGTGATCCC
TCATGGCGGCAGCGTGTCGCACAAGGACATGTAAATGGATTAGCTCGAGCTTTCCAACTTCAGCCAAAGGATGATGCGCC
CGATCCTTCTCCTAACTTCTTTAAAGTTATTGCCGGGTCATTCAAACAACGAATTAACGCAGAGGAACGTGTAGATCTTC
TAACGGACGAGGATATGGAAGCAATTATTGTACCAGTAACCATATCTGGACAACAGTGGTATCGTGTTCAATCCGGTGCA
TTTCGTGATCGCGACAATGCTAATGCCAGAATAAATCAATTACAAGATCTAGGAATTGAAGCATTCATTATCACAGATTA
A

Upstream 100 bases:

>100_bases
CGTGGATACACAGAAGTGGATTTATACAACACTCATGTTGTTTGTCCACGATTTTCTATTCATTATCCATACACTATAAT
CGAGAGGAGGAAATGAACTA

Downstream 100 bases:

>100_bases
AAAAACAACTGGAGCACTATTTGAATAACTACTTCATACTTCACTGTCTTGCCTCCAAACAAAGATAGTTTAAGTAGAGA
TTCTTTAGTCGCTTTCCAGT

Product: sporulation specific N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MKLYLDPGHGGSDPGAVGNGLQEKTINLDIAIRIRDLLLSNYNNVSVKMSRTSDSTKSLSQRTNEANNWNADYFLSIHCN
SFNGSAYGYEDFIHNSLSDQSTTARYQNIIHEEVSKVNQLRNRGKKKANFHVLRESHMPALLTENGFIDHSGDAAKLRDP
SWRQRVAQGHVNGLARAFQLQPKDDAPDPSPNFFKVIAGSFKQRINAEERVDLLTDEDMEAIIVPVTISGQQWYRVQSGA
FRDRDNANARINQLQDLGIEAFIITD

Sequences:

>Translated_266_residues
MKLYLDPGHGGSDPGAVGNGLQEKTINLDIAIRIRDLLLSNYNNVSVKMSRTSDSTKSLSQRTNEANNWNADYFLSIHCN
SFNGSAYGYEDFIHNSLSDQSTTARYQNIIHEEVSKVNQLRNRGKKKANFHVLRESHMPALLTENGFIDHSGDAAKLRDP
SWRQRVAQGHVNGLARAFQLQPKDDAPDPSPNFFKVIAGSFKQRINAEERVDLLTDEDMEAIIVPVTISGQQWYRVQSGA
FRDRDNANARINQLQDLGIEAFIITD
>Mature_266_residues
MKLYLDPGHGGSDPGAVGNGLQEKTINLDIAIRIRDLLLSNYNNVSVKMSRTSDSTKSLSQRTNEANNWNADYFLSIHCN
SFNGSAYGYEDFIHNSLSDQSTTARYQNIIHEEVSKVNQLRNRGKKKANFHVLRESHMPALLTENGFIDHSGDAAKLRDP
SWRQRVAQGHVNGLARAFQLQPKDDAPDPSPNFFKVIAGSFKQRINAEERVDLLTDEDMEAIIVPVTISGQQWYRVQSGA
FRDRDNANARINQLQDLGIEAFIITD

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=219, Percent_Identity=28.310502283105, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788776, Length=221, Percent_Identity=26.6968325791855, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 29891; Mature: 29891

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYLDPGHGGSDPGAVGNGLQEKTINLDIAIRIRDLLLSNYNNVSVKMSRTSDSTKSLS
CEEEECCCCCCCCCCHHCCCCCCEEEEEEEEEEEEHHHHCCCCCEEEEEECCCCHHHHHH
QRTNEANNWNADYFLSIHCNSFNGSAYGYEDFIHNSLSDQSTTARYQNIIHEEVSKVNQL
HHCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RNRGKKKANFHVLRESHMPALLTENGFIDHSGDAAKLRDPSWRQRVAQGHVNGLARAFQL
HHCCCCCCCEEEEECCCCCEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEC
QPKDDAPDPSPNFFKVIAGSFKQRINAEERVDLLTDEDMEAIIVPVTISGQQWYRVQSGA
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEEEECCCEEEEECCCC
FRDRDNANARINQLQDLGIEAFIITD
CCCCCCCCHHHHHHHHCCEEEEEEEC
>Mature Secondary Structure
MKLYLDPGHGGSDPGAVGNGLQEKTINLDIAIRIRDLLLSNYNNVSVKMSRTSDSTKSLS
CEEEECCCCCCCCCCHHCCCCCCEEEEEEEEEEEEHHHHCCCCCEEEEEECCCCHHHHHH
QRTNEANNWNADYFLSIHCNSFNGSAYGYEDFIHNSLSDQSTTARYQNIIHEEVSKVNQL
HHCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RNRGKKKANFHVLRESHMPALLTENGFIDHSGDAAKLRDPSWRQRVAQGHVNGLARAFQL
HHCCCCCCCEEEEECCCCCEEEECCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHEEC
QPKDDAPDPSPNFFKVIAGSFKQRINAEERVDLLTDEDMEAIIVPVTISGQQWYRVQSGA
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEEEEEECCCEEEEECCCC
FRDRDNANARINQLQDLGIEAFIITD
CCCCCCCCHHHHHHHHCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]