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The map label for this gene is yafV [C]

Identifier: 23100566

GI number: 23100566

Start: 3239564

End: 3240349

Strand: Reverse

Name: yafV [C]

Synonym: OB3111

Alternate gene names: 23100566

Gene position: 3240349-3239564 (Counterclockwise)

Preceding gene: 23100568

Following gene: 23100564

Centisome position: 89.25

GC content: 36.01

Gene sequence:

>786_bases
ATGAGACATGCGATATATCAAATGGATATTGTACCAGGAAATCCAGAAGCAAATCGAACAAAGGTGAAAACATGGATAGA
ACAAACAGTTTCCAAGAACGATATTGATATCATAGTGCTTCCGGAAATGTGGACAACGGGATATACGCTTGCCGATTTAA
ATGAAGTAGCTGATGAGGGAGAAGAACCGACTTGTTCATTTTTACAACAGTTAGCTAGAGAAAATCAAGTGCATATCATA
GCTGGTTCCATAGCAAATAAACGGAATGGAAAAATATATAATACCGCAATTGTTGTAAATCGCAACGGAGAAAAGGTTTA
TACGTACGATAAGATTCATTTAGTTCCAATGCTAGATGAGCCTACTTATTTAAATGGTGGGGAAGAGAAGGTACGTACAT
TTGAATTAGACGGTATAAAAATGGGATTAATTATTTGTTATGATCTTCGTTTTCCAGAACTTACTCGTTCCTTAGCAGTA
TCCGGAGCTGAAGTAATATATATTGTGGCAGAATGGCCATCTGCAAGAAAAGATCATTGGAAAAACTTACAACTAGCAAG
AGCGATTGAGAACCAATGTTTTATTATTTCGGCCAATCGAGTCGGTATATTTAATAATGTGGAATTTTGTGGACTATCCT
TGGTAATTAATCCATGGGGAGATATACGTACATTAGGTTCAGAAAATCACGAGGAAACGATTGTTGATAATATTGATCTA
CAGAAAGTAAGGGAAGCCAGAAAGAATGTACCAATTTTTGAAAGTAGGGTTCCGGAACTATATTAA

Upstream 100 bases:

>100_bases
GTCTGATTGGGATACAAATAAGCAGCATATTCATGTATCTGATTTTGAAAATTTTACAACTTTGATACAATAAAACAAAT
GGAGATAGGGGATGGGTAGA

Downstream 100 bases:

>100_bases
AATCAATCAATTTCAAAGTTGCTAAGTTTTGGTTGAACCAAGTGACCATTTTTCCAAAATAAAGATCCAAACGATTAATC
GGAATGAGCGACTAATGGCT

Product: hypothetical protein

Products: NA

Alternate protein names: ORF 5 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MRHAIYQMDIVPGNPEANRTKVKTWIEQTVSKNDIDIIVLPEMWTTGYTLADLNEVADEGEEPTCSFLQQLARENQVHII
AGSIANKRNGKIYNTAIVVNRNGEKVYTYDKIHLVPMLDEPTYLNGGEEKVRTFELDGIKMGLIICYDLRFPELTRSLAV
SGAEVIYIVAEWPSARKDHWKNLQLARAIENQCFIISANRVGIFNNVEFCGLSLVINPWGDIRTLGSENHEETIVDNIDL
QKVREARKNVPIFESRVPELY

Sequences:

>Translated_261_residues
MRHAIYQMDIVPGNPEANRTKVKTWIEQTVSKNDIDIIVLPEMWTTGYTLADLNEVADEGEEPTCSFLQQLARENQVHII
AGSIANKRNGKIYNTAIVVNRNGEKVYTYDKIHLVPMLDEPTYLNGGEEKVRTFELDGIKMGLIICYDLRFPELTRSLAV
SGAEVIYIVAEWPSARKDHWKNLQLARAIENQCFIISANRVGIFNNVEFCGLSLVINPWGDIRTLGSENHEETIVDNIDL
QKVREARKNVPIFESRVPELY
>Mature_261_residues
MRHAIYQMDIVPGNPEANRTKVKTWIEQTVSKNDIDIIVLPEMWTTGYTLADLNEVADEGEEPTCSFLQQLARENQVHII
AGSIANKRNGKIYNTAIVVNRNGEKVYTYDKIHLVPMLDEPTYLNGGEEKVRTFELDGIKMGLIICYDLRFPELTRSLAV
SGAEVIYIVAEWPSARKDHWKNLQLARAIENQCFIISANRVGIFNNVEFCGLSLVINPWGDIRTLGSENHEETIVDNIDL
QKVREARKNVPIFESRVPELY

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=272, Percent_Identity=31.9852941176471, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI5031947, Length=213, Percent_Identity=34.7417840375587, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI297632350, Length=213, Percent_Identity=34.7417840375587, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI297632348, Length=213, Percent_Identity=34.7417840375587, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI1786412, Length=221, Percent_Identity=33.9366515837104, Blast_Score=116, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17556280, Length=280, Percent_Identity=28.9285714285714, Blast_Score=104, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6323383, Length=274, Percent_Identity=28.8321167883212, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322335, Length=210, Percent_Identity=27.1428571428571, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355835, Length=275, Percent_Identity=32, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17933642, Length=258, Percent_Identity=27.906976744186, Blast_Score=100, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29748; Mature: 29748

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHAIYQMDIVPGNPEANRTKVKTWIEQTVSKNDIDIIVLPEMWTTGYTLADLNEVADEG
CCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCC
EEPTCSFLQQLARENQVHIIAGSIANKRNGKIYNTAIVVNRNGEKVYTYDKIHLVPMLDE
CCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEEECCCCEEEEEEEEEEEEECCC
PTYLNGGEEKVRTFELDGIKMGLIICYDLRFPELTRSLAVSGAEVIYIVAEWPSARKDHW
CCCCCCCCHHEEEEEECCEEEEEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCHHHH
KNLQLARAIENQCFIISANRVGIFNNVEFCGLSLVINPWGDIRTLGSENHEETIVDNIDL
CCHHHHHHHCCCEEEEECCCEEEECCCCEEEEEEEECCCCCEEECCCCCCCCHHHCCCCH
QKVREARKNVPIFESRVPELY
HHHHHHHHCCCCHHCCCCCCC
>Mature Secondary Structure
MRHAIYQMDIVPGNPEANRTKVKTWIEQTVSKNDIDIIVLPEMWTTGYTLADLNEVADEG
CCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCC
EEPTCSFLQQLARENQVHIIAGSIANKRNGKIYNTAIVVNRNGEKVYTYDKIHLVPMLDE
CCCHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEEEECCCCEEEEEEEEEEEEECCC
PTYLNGGEEKVRTFELDGIKMGLIICYDLRFPELTRSLAVSGAEVIYIVAEWPSARKDHW
CCCCCCCCHHEEEEEECCEEEEEEEEEECCCHHHHHHHHCCCCEEEEEEECCCCCCHHHH
KNLQLARAIENQCFIISANRVGIFNNVEFCGLSLVINPWGDIRTLGSENHEETIVDNIDL
CCHHHHHHHCCCEEEEECCCEEEECCCCEEEEEEEECCCCCEEECCCCCCCCHHHCCCCH
QKVREARKNVPIFESRVPELY
HHHHHHHHCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7565609 [H]