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The map label for this gene is mrpA [H]

Identifier: 23100564

GI number: 23100564

Start: 3235779

End: 3238196

Strand: Reverse

Name: mrpA [H]

Synonym: OB3109

Alternate gene names: 23100564

Gene position: 3238196-3235779 (Counterclockwise)

Preceding gene: 23100566

Following gene: 23100563

Centisome position: 89.19

GC content: 37.18

Gene sequence:

>2418_bases
TTGACAACATTGATTATTGCAATAATCTTTCCTTTTATTGCAGCAGCAATTATACCTTTCCTTTACAAATATGTACGGCG
AATACATGTCGGTTGGTTTGCATTAGTCGTATCTACTGGTTTATTTATTTATTTTTCTCAATTTATCCCTACTATAGCTA
ATGGAGATGTAATCACCATCACCTTAAATTGGATACCGTCTTACGGAATTAACATTACATCTTATCTAGATGGATTAAGC
TTGACTTTTTCCCTACTAATATCTGGTGTAGGTGCACTCGTCATTCTATATTCGATTTATTACCTTTCATCCAAAGAATC
ACTGGGTCACTTCTATACATACCTTATGTTATTTATGGGGGCAATGTTAGGAGTGGTAACATCCGATAACATGCTAGTTT
TATATGTATTTTGGGAACTGACAAGTATATCTTCGTTCCTTTTAATAGCTTTTTGGTACCAACGGAAGCGGTCGAGAGAT
GGGGCGAAAAAATCATTACTAATCACCGTTACTGGTGGTATAGCCATGCTAGTCGGATTCATTATGGTTGGTCAAATGTC
TGGCACGTGGTCAATCCGGGAAGTAATTGGTAATGTTAATTTATTTAATAATCATTATCTGTTTATTGCGGCGATGATTT
TAGTATTGGTCGGTGCATTTACAAAATCTGCTCAATTCCCATTTCATATATGGTTACCTGATGCGATGGAAGCACCTACT
CCGGTTAGTGCATATTTGCATTCAGCGACAATGGTAAAAGCAGGAATCTATCTTGTTGCTCGATTTACTCCTGTTTTTGG
TGGAGAATCTATTTGGTTCTGGGCGGTAACAGGTGTAGGGTTATTCACGCTGTTTTGGGGTTCTTTTAATGCAGTTCGAC
AGACAGATTTGAAGGCATTACTCGCATATTCTACAATTAGCCAGCTTGGGATGATTATGAGTATGTTTGGTATTGGTTCT
GCCGCATTAAATTTAGGGTACTCCACAGAATCGGTTGCATTTACAAGTGCTTCTTTTGCTGCATTATTCCATCTCGTAAA
TCACTCTACGTTTAAAGGAGCTCTATTTATGGTGGTAGGTATTGTCGACCATAGTGTAGGGACTCGAGATATACGTCGAC
TTGGTGGATTAATTACTCTGATGCCTGTTACATTCTCTATTGCGTTAATTGGAAGCTTGTCTATGGCAGGTTTACCGCCA
TTAAACGGTTTTTTAAGTAAAGAAATGTTCTTCATGGCAGTTGTTAATATAAGTGAAGCAGAATTTTTCGCTCTAGAAAC
TTGGGGGATCCTGCTGCCGATAATTGCTTGGGTTGCAAGTGTATTTACGTTTGTGTATTCCATGATACTCGTTATAAAGA
CTTTCCTAGGTCCATATCAAGAAGAAAAATTAGAACATCCTGCACATGAACCAAAACCAGGGATGCTGGTTTCACCAATT
ATATTAGCTTTATTAGTCATTGGTATTTTCTTCTTCCCGAATATAATTGGAAAGAATTTAATTACACCCGCAATGGCTGG
GATATTTCCGAATCTTGCCGCTGAAGGTGCAGAGCTAAGCGAGTCTATATATGCATGGCATGGCTTTGAACCTGAATTAT
TTATGACGATCGGAGTGGTGCTTTTTGGTTTGGCATTATTCTTTACTAAGAGGTATTGGGTAGGAGTGTATAAACTATTT
CCACAAAAATGGTCATTTAATGTATTCTATAGCTTTATGTTAGATCAAGTAGAGGAAAAATCGACTGGATTCACAAATGC
GTATATGACACAAAATTTAAGAGACTATTTAGTATATATTTATTTATTCTTTATTATCGCTATAGGCGGAATCCTAATTT
ATACCGAAGCATATCAATTTGAATTGCCTACCAATGCTGCGATTTCTAATTTTGAGCTGATCACTGCTCTAGCAATGATG
TCAGCGGCGATCGCAATCCTATTTGCGAAATCACGACTTACTGCAATTCTGTTAAATAGTATTCTAGGGTTTGGAGTAGC
GATATTCTTTGTATTATTTAGAGCACCAGATTTAGCTCTAACACAAACAATTGTAGAATCTGTTACCACTGTTCTATTTT
TAGCAGCGTACTACTTTCTCCCTGAATGGAAGAAAGAAGATACCCCGAAGAAAACACGAGGTACTAATTTGGTCATTTCC
ATTTGTGTTGGTGTGGTATTTACAGTCATTGCATTAATGGTCCAAAGTGGAAAAACATTTGAATCCATTTCGAAATTCTT
CGAGGACTCATATGAGCTTGCTGGTGGAAAGAATATGGTAAATGCTATTCTCGGTGATTTTCGTGCATTAGACACGATGC
TGGAGGTAATAGTCCTACTTATTGCCGGGCTAGGTGTCTATATATTTATTAAATTAAAGAATAACAATAAGGAGGGTGAG
CAGTATGAAGATAAATAA

Upstream 100 bases:

>100_bases
TCACAACAAATGGGTGTCATAGATATTCATTAAATAGTATATCCTGTTAACACTTTTGTGTGATAACAGTAAAGTTAACC
TATTGATTGGAGGTTACAGT

Downstream 100 bases:

>100_bases
CGTTATTCTTCGTACCGTTGCAAAACTTGTTTCATTTATCATACTCACCCTTGCAGTTTATTTGTTTCTTTCTGGACATC
ATAGTCCGGGAGGTGGCTTC

Product: monovalent cation/H+ antiporter subunit A

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit A; Multiple resistance and pH homeostasis protein A [H]

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MTTLIIAIIFPFIAAAIIPFLYKYVRRIHVGWFALVVSTGLFIYFSQFIPTIANGDVITITLNWIPSYGINITSYLDGLS
LTFSLLISGVGALVILYSIYYLSSKESLGHFYTYLMLFMGAMLGVVTSDNMLVLYVFWELTSISSFLLIAFWYQRKRSRD
GAKKSLLITVTGGIAMLVGFIMVGQMSGTWSIREVIGNVNLFNNHYLFIAAMILVLVGAFTKSAQFPFHIWLPDAMEAPT
PVSAYLHSATMVKAGIYLVARFTPVFGGESIWFWAVTGVGLFTLFWGSFNAVRQTDLKALLAYSTISQLGMIMSMFGIGS
AALNLGYSTESVAFTSASFAALFHLVNHSTFKGALFMVVGIVDHSVGTRDIRRLGGLITLMPVTFSIALIGSLSMAGLPP
LNGFLSKEMFFMAVVNISEAEFFALETWGILLPIIAWVASVFTFVYSMILVIKTFLGPYQEEKLEHPAHEPKPGMLVSPI
ILALLVIGIFFFPNIIGKNLITPAMAGIFPNLAAEGAELSESIYAWHGFEPELFMTIGVVLFGLALFFTKRYWVGVYKLF
PQKWSFNVFYSFMLDQVEEKSTGFTNAYMTQNLRDYLVYIYLFFIIAIGGILIYTEAYQFELPTNAAISNFELITALAMM
SAAIAILFAKSRLTAILLNSILGFGVAIFFVLFRAPDLALTQTIVESVTTVLFLAAYYFLPEWKKEDTPKKTRGTNLVIS
ICVGVVFTVIALMVQSGKTFESISKFFEDSYELAGGKNMVNAILGDFRALDTMLEVIVLLIAGLGVYIFIKLKNNNKEGE
QYEDK

Sequences:

>Translated_805_residues
MTTLIIAIIFPFIAAAIIPFLYKYVRRIHVGWFALVVSTGLFIYFSQFIPTIANGDVITITLNWIPSYGINITSYLDGLS
LTFSLLISGVGALVILYSIYYLSSKESLGHFYTYLMLFMGAMLGVVTSDNMLVLYVFWELTSISSFLLIAFWYQRKRSRD
GAKKSLLITVTGGIAMLVGFIMVGQMSGTWSIREVIGNVNLFNNHYLFIAAMILVLVGAFTKSAQFPFHIWLPDAMEAPT
PVSAYLHSATMVKAGIYLVARFTPVFGGESIWFWAVTGVGLFTLFWGSFNAVRQTDLKALLAYSTISQLGMIMSMFGIGS
AALNLGYSTESVAFTSASFAALFHLVNHSTFKGALFMVVGIVDHSVGTRDIRRLGGLITLMPVTFSIALIGSLSMAGLPP
LNGFLSKEMFFMAVVNISEAEFFALETWGILLPIIAWVASVFTFVYSMILVIKTFLGPYQEEKLEHPAHEPKPGMLVSPI
ILALLVIGIFFFPNIIGKNLITPAMAGIFPNLAAEGAELSESIYAWHGFEPELFMTIGVVLFGLALFFTKRYWVGVYKLF
PQKWSFNVFYSFMLDQVEEKSTGFTNAYMTQNLRDYLVYIYLFFIIAIGGILIYTEAYQFELPTNAAISNFELITALAMM
SAAIAILFAKSRLTAILLNSILGFGVAIFFVLFRAPDLALTQTIVESVTTVLFLAAYYFLPEWKKEDTPKKTRGTNLVIS
ICVGVVFTVIALMVQSGKTFESISKFFEDSYELAGGKNMVNAILGDFRALDTMLEVIVLLIAGLGVYIFIKLKNNNKEGE
QYEDK
>Mature_804_residues
TTLIIAIIFPFIAAAIIPFLYKYVRRIHVGWFALVVSTGLFIYFSQFIPTIANGDVITITLNWIPSYGINITSYLDGLSL
TFSLLISGVGALVILYSIYYLSSKESLGHFYTYLMLFMGAMLGVVTSDNMLVLYVFWELTSISSFLLIAFWYQRKRSRDG
AKKSLLITVTGGIAMLVGFIMVGQMSGTWSIREVIGNVNLFNNHYLFIAAMILVLVGAFTKSAQFPFHIWLPDAMEAPTP
VSAYLHSATMVKAGIYLVARFTPVFGGESIWFWAVTGVGLFTLFWGSFNAVRQTDLKALLAYSTISQLGMIMSMFGIGSA
ALNLGYSTESVAFTSASFAALFHLVNHSTFKGALFMVVGIVDHSVGTRDIRRLGGLITLMPVTFSIALIGSLSMAGLPPL
NGFLSKEMFFMAVVNISEAEFFALETWGILLPIIAWVASVFTFVYSMILVIKTFLGPYQEEKLEHPAHEPKPGMLVSPII
LALLVIGIFFFPNIIGKNLITPAMAGIFPNLAAEGAELSESIYAWHGFEPELFMTIGVVLFGLALFFTKRYWVGVYKLFP
QKWSFNVFYSFMLDQVEEKSTGFTNAYMTQNLRDYLVYIYLFFIIAIGGILIYTEAYQFELPTNAAISNFELITALAMMS
AAIAILFAKSRLTAILLNSILGFGVAIFFVLFRAPDLALTQTIVESVTTVLFLAAYYFLPEWKKEDTPKKTRGTNLVISI
CVGVVFTVIALMVQSGKTFESISKFFEDSYELAGGKNMVNAILGDFRALDTMLEVIVLLIAGLGVYIFIKLKNNNKEGEQ
YEDK

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit A family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=413, Percent_Identity=33.1719128329298, Blast_Score=194, Evalue=4e-49,
Organism=Escherichia coli, GI1788614, Length=550, Percent_Identity=30.7272727272727, Blast_Score=192, Evalue=6e-50,
Organism=Escherichia coli, GI1788829, Length=455, Percent_Identity=31.8681318681319, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI1788831, Length=357, Percent_Identity=33.0532212885154, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1788827, Length=432, Percent_Identity=30.3240740740741, Blast_Score=139, Evalue=1e-33,
Organism=Escherichia coli, GI1788613, Length=430, Percent_Identity=25.8139534883721, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI2367154, Length=469, Percent_Identity=25.5863539445629, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI145693160, Length=406, Percent_Identity=26.1083743842365, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005663
- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 89338; Mature: 89207

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLIIAIIFPFIAAAIIPFLYKYVRRIHVGWFALVVSTGLFIYFSQFIPTIANGDVITI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TLNWIPSYGINITSYLDGLSLTFSLLISGVGALVILYSIYYLSSKESLGHFYTYLMLFMG
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AMLGVVTSDNMLVLYVFWELTSISSFLLIAFWYQRKRSRDGAKKSLLITVTGGIAMLVGF
HHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHH
IMVGQMSGTWSIREVIGNVNLFNNHYLFIAAMILVLVGAFTKSAQFPFHIWLPDAMEAPT
HHHHCCCCCHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
PVSAYLHSATMVKAGIYLVARFTPVFGGESIWFWAVTGVGLFTLFWGSFNAVRQTDLKAL
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHH
LAYSTISQLGMIMSMFGIGSAALNLGYSTESVAFTSASFAALFHLVNHSTFKGALFMVVG
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHH
IVDHSVGTRDIRRLGGLITLMPVTFSIALIGSLSMAGLPPLNGFLSKEMFFMAVVNISEA
HHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCH
EFFALETWGILLPIIAWVASVFTFVYSMILVIKTFLGPYQEEKLEHPAHEPKPGMLVSPI
HEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHH
ILALLVIGIFFFPNIIGKNLITPAMAGIFPNLAAEGAELSESIYAWHGFEPELFMTIGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
LFGLALFFTKRYWVGVYKLFPQKWSFNVFYSFMLDQVEEKSTGFTNAYMTQNLRDYLVYI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
YLFFIIAIGGILIYTEAYQFELPTNAAISNFELITALAMMSAAIAILFAKSRLTAILLNS
HHHHHHHHCHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGFGVAIFFVLFRAPDLALTQTIVESVTTVLFLAAYYFLPEWKKEDTPKKTRGTNLVIS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
ICVGVVFTVIALMVQSGKTFESISKFFEDSYELAGGKNMVNAILGDFRALDTMLEVIVLL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IAGLGVYIFIKLKNNNKEGEQYEDK
HHCCCEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TTLIIAIIFPFIAAAIIPFLYKYVRRIHVGWFALVVSTGLFIYFSQFIPTIANGDVITI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
TLNWIPSYGINITSYLDGLSLTFSLLISGVGALVILYSIYYLSSKESLGHFYTYLMLFMG
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
AMLGVVTSDNMLVLYVFWELTSISSFLLIAFWYQRKRSRDGAKKSLLITVTGGIAMLVGF
HHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHH
IMVGQMSGTWSIREVIGNVNLFNNHYLFIAAMILVLVGAFTKSAQFPFHIWLPDAMEAPT
HHHHCCCCCHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCC
PVSAYLHSATMVKAGIYLVARFTPVFGGESIWFWAVTGVGLFTLFWGSFNAVRQTDLKAL
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHH
LAYSTISQLGMIMSMFGIGSAALNLGYSTESVAFTSASFAALFHLVNHSTFKGALFMVVG
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHH
IVDHSVGTRDIRRLGGLITLMPVTFSIALIGSLSMAGLPPLNGFLSKEMFFMAVVNISEA
HHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEECCCH
EFFALETWGILLPIIAWVASVFTFVYSMILVIKTFLGPYQEEKLEHPAHEPKPGMLVSPI
HEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCHHHH
ILALLVIGIFFFPNIIGKNLITPAMAGIFPNLAAEGAELSESIYAWHGFEPELFMTIGVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
LFGLALFFTKRYWVGVYKLFPQKWSFNVFYSFMLDQVEEKSTGFTNAYMTQNLRDYLVYI
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
YLFFIIAIGGILIYTEAYQFELPTNAAISNFELITALAMMSAAIAILFAKSRLTAILLNS
HHHHHHHHCHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGFGVAIFFVLFRAPDLALTQTIVESVTTVLFLAAYYFLPEWKKEDTPKKTRGTNLVIS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
ICVGVVFTVIALMVQSGKTFESISKFFEDSYELAGGKNMVNAILGDFRALDTMLEVIVLL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IAGLGVYIFIKLKNNNKEGEQYEDK
HHCCCEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10198001; 11356194 [H]