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The map label for this gene is mrpD [H]

Identifier: 23100561

GI number: 23100561

Start: 3233555

End: 3235036

Strand: Reverse

Name: mrpD [H]

Synonym: OB3106

Alternate gene names: 23100561

Gene position: 3235036-3233555 (Counterclockwise)

Preceding gene: 23100562

Following gene: 23100560

Centisome position: 89.11

GC content: 36.17

Gene sequence:

>1482_bases
ATGAATAATATTATTGCAATGCCTATGATGATCCCTATACTTGTTGGAATTCTGTTAATATTTTTCCGTCATCACATTAA
ACTGCAACGTTGGAGTAGTTTAGCTGCGATGATTGCCAATATCGGAATCACTGCATATATCTTACATCAAATTCAACAAA
ATGGAATTCTCCGTCTAGATTTCGGCGGATGGGAGCCGCCATTTGGGATATTATTTGTGGCTGATTCATTTTCTGCGCTA
CTTGTGTTAACTACAAGTATTGTTGCTGCAATATGTCTTGTTTATGCTTTTTCTTCAACAGAAAAGTCTTATGAGCAGAT
GTTCTTTTACCCTTTTATTAATTTTTTAGTCGCAGGAGTAAACGGTTCTTTCTTAACAGGGGATCTATTTAATTTATATG
TGTGTTTTGAAATTATGTTGTTAGCCTCTTATGTGTTAATTGCATTTGGCGGTCGTAAAGGACAGCTTAGAGAATCGATT
AAATATGTCATTATAAATGTTGTATCGTCTTGGTTCTTTTTAATTGCGATTGCTTATACGTATGGCGCATTAGGAACACT
AAATTTTGCGCATCTTTCTGTACGAATAGCTGAACAAGGTCAGTCGCCGATGCTTACTGTCGTGAGTATTGTATTCCTGA
CAGTATTTGCTTTAAAAGCAGGATTATTATTATATTTCTGGTTACCGGGGTCCTATAGTGTTCCACCGACTGCAATCGCT
GCTTTATTTGGAGCTTTATTAACGAAGGTAGGAATATATGCGATGTTTCGCGTGTTTACGCTACTGTTTTATCATGAACC
ACACATTACGCATACAATTATTGGCATATTGGCAGGATTGACGTTAATAATTGGAAGTATTGGAGCAGTAGCATACAAGG
ATGTGCGTTTAATCGCTTCATACAATGTTGTTATTGCTGTTGGTTTTATATTGGTAGGGTTAGCTGTTGCAACACCGACT
GCGATGGAAGGCTCCATCTTTTATTTAATCCATGACATGATTGTAAAAGCACTCTTATTCTTACTTGCAGGAACGATGAT
TGCTGTTACAGGGGAGACAAGAATAGATTATATGAGTGGACTAATTCGAAATTATCCTTTACTTGGTTGGATTTTCTTCA
TTGTTATACTTGCATTAGCAGGTATCCCGCCTTTAAGTGGCTTTATTGGAAAAGTCTTAGTTGGCCAAGGAGCTGTCGAG
GCTGGGGCATATATTTTACTCGCTTTAGCGTTTATTTCGAGTATTTTTGTTCTATATTCATTAATGCGTGTATTCCTTAC
TAGCTTTTGGGGAGAAACGATTATAAGTAAAGAAGATAAAATTCCAATGAAAAATGGCTGGATTATTCCATGTATAATAT
TAGCAGCAGGATCTTTTATTCTTGGTATCGGAGTTGAACCTTTAACTGTTTATGTAAAAGATGCAGCGGAAGTTTTAGCG
AATCCACAAATTTATATTGATGCGGTTTTATATGATAACTAA

Upstream 100 bases:

>100_bases
GTGATTAGCTTTGCTGTAACAGCTGTGTCCTTAGTATTAGCTTACCGTACGTACCAGGAAACAGGAACAGACAAATTGGA
TCAGTTACGGGGGGATCGGA

Downstream 100 bases:

>100_bases
GAATTGTCTTAGAGGAGGTGGATTATAAATGCCCGCCCAGTTTTTATTAAATGTGTTTATTGCAGTATTATGGACCTTAA
TAAGCGATGAAGATGAATTA

Product: putative monovalent cation/H+ antiporter subunit D

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MNNIIAMPMMIPILVGILLIFFRHHIKLQRWSSLAAMIANIGITAYILHQIQQNGILRLDFGGWEPPFGILFVADSFSAL
LVLTTSIVAAICLVYAFSSTEKSYEQMFFYPFINFLVAGVNGSFLTGDLFNLYVCFEIMLLASYVLIAFGGRKGQLRESI
KYVIINVVSSWFFLIAIAYTYGALGTLNFAHLSVRIAEQGQSPMLTVVSIVFLTVFALKAGLLLYFWLPGSYSVPPTAIA
ALFGALLTKVGIYAMFRVFTLLFYHEPHITHTIIGILAGLTLIIGSIGAVAYKDVRLIASYNVVIAVGFILVGLAVATPT
AMEGSIFYLIHDMIVKALLFLLAGTMIAVTGETRIDYMSGLIRNYPLLGWIFFIVILALAGIPPLSGFIGKVLVGQGAVE
AGAYILLALAFISSIFVLYSLMRVFLTSFWGETIISKEDKIPMKNGWIIPCIILAAGSFILGIGVEPLTVYVKDAAEVLA
NPQIYIDAVLYDN

Sequences:

>Translated_493_residues
MNNIIAMPMMIPILVGILLIFFRHHIKLQRWSSLAAMIANIGITAYILHQIQQNGILRLDFGGWEPPFGILFVADSFSAL
LVLTTSIVAAICLVYAFSSTEKSYEQMFFYPFINFLVAGVNGSFLTGDLFNLYVCFEIMLLASYVLIAFGGRKGQLRESI
KYVIINVVSSWFFLIAIAYTYGALGTLNFAHLSVRIAEQGQSPMLTVVSIVFLTVFALKAGLLLYFWLPGSYSVPPTAIA
ALFGALLTKVGIYAMFRVFTLLFYHEPHITHTIIGILAGLTLIIGSIGAVAYKDVRLIASYNVVIAVGFILVGLAVATPT
AMEGSIFYLIHDMIVKALLFLLAGTMIAVTGETRIDYMSGLIRNYPLLGWIFFIVILALAGIPPLSGFIGKVLVGQGAVE
AGAYILLALAFISSIFVLYSLMRVFLTSFWGETIISKEDKIPMKNGWIIPCIILAAGSFILGIGVEPLTVYVKDAAEVLA
NPQIYIDAVLYDN
>Mature_493_residues
MNNIIAMPMMIPILVGILLIFFRHHIKLQRWSSLAAMIANIGITAYILHQIQQNGILRLDFGGWEPPFGILFVADSFSAL
LVLTTSIVAAICLVYAFSSTEKSYEQMFFYPFINFLVAGVNGSFLTGDLFNLYVCFEIMLLASYVLIAFGGRKGQLRESI
KYVIINVVSSWFFLIAIAYTYGALGTLNFAHLSVRIAEQGQSPMLTVVSIVFLTVFALKAGLLLYFWLPGSYSVPPTAIA
ALFGALLTKVGIYAMFRVFTLLFYHEPHITHTIIGILAGLTLIIGSIGAVAYKDVRLIASYNVVIAVGFILVGLAVATPT
AMEGSIFYLIHDMIVKALLFLLAGTMIAVTGETRIDYMSGLIRNYPLLGWIFFIVILALAGIPPLSGFIGKVLVGQGAVE
AGAYILLALAFISSIFVLYSLMRVFLTSFWGETIISKEDKIPMKNGWIIPCIILAAGSFILGIGVEPLTVYVKDAAEVLA
NPQIYIDAVLYDN

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=339, Percent_Identity=24.4837758112094, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI251831117, Length=341, Percent_Identity=24.9266862170088, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788827, Length=350, Percent_Identity=26.8571428571429, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788614, Length=463, Percent_Identity=25.2699784017279, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788829, Length=406, Percent_Identity=24.384236453202, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788613, Length=403, Percent_Identity=26.5508684863524, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788831, Length=275, Percent_Identity=22.9090909090909, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693160, Length=441, Percent_Identity=24.9433106575964, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI2367154, Length=296, Percent_Identity=24.3243243243243, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 53953; Mature: 53953

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNIIAMPMMIPILVGILLIFFRHHIKLQRWSSLAAMIANIGITAYILHQIQQNGILRLD
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEE
FGGWEPPFGILFVADSFSALLVLTTSIVAAICLVYAFSSTEKSYEQMFFYPFINFLVAGV
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
NGSFLTGDLFNLYVCFEIMLLASYVLIAFGGRKGQLRESIKYVIINVVSSWFFLIAIAYT
CCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGALGTLNFAHLSVRIAEQGQSPMLTVVSIVFLTVFALKAGLLLYFWLPGSYSVPPTAIA
HHHHHCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHH
ALFGALLTKVGIYAMFRVFTLLFYHEPHITHTIIGILAGLTLIIGSIGAVAYKDVRLIAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNVVIAVGFILVGLAVATPTAMEGSIFYLIHDMIVKALLFLLAGTMIAVTGETRIDYMSG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHEEEECCCHHHHHHH
LIRNYPLLGWIFFIVILALAGIPPLSGFIGKVLVGQGAVEAGAYILLALAFISSIFVLYS
HHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LMRVFLTSFWGETIISKEDKIPMKNGWIIPCIILAAGSFILGIGVEPLTVYVKDAAEVLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHC
NPQIYIDAVLYDN
CCCEEEEEEEECC
>Mature Secondary Structure
MNNIIAMPMMIPILVGILLIFFRHHIKLQRWSSLAAMIANIGITAYILHQIQQNGILRLD
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCEEEEE
FGGWEPPFGILFVADSFSALLVLTTSIVAAICLVYAFSSTEKSYEQMFFYPFINFLVAGV
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
NGSFLTGDLFNLYVCFEIMLLASYVLIAFGGRKGQLRESIKYVIINVVSSWFFLIAIAYT
CCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGALGTLNFAHLSVRIAEQGQSPMLTVVSIVFLTVFALKAGLLLYFWLPGSYSVPPTAIA
HHHHHCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHH
ALFGALLTKVGIYAMFRVFTLLFYHEPHITHTIIGILAGLTLIIGSIGAVAYKDVRLIAS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YNVVIAVGFILVGLAVATPTAMEGSIFYLIHDMIVKALLFLLAGTMIAVTGETRIDYMSG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHEEEECCCHHHHHHH
LIRNYPLLGWIFFIVILALAGIPPLSGFIGKVLVGQGAVEAGAYILLALAFISSIFVLYS
HHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
LMRVFLTSFWGETIISKEDKIPMKNGWIIPCIILAAGSFILGIGVEPLTVYVKDAAEVLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHC
NPQIYIDAVLYDN
CCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10568751; 10198001; 11356194 [H]