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The map label for this gene is yrvJ [H]

Identifier: 23100540

GI number: 23100540

Start: 3207512

End: 3208651

Strand: Direct

Name: yrvJ [H]

Synonym: OB3085

Alternate gene names: 23100540

Gene position: 3207512-3208651 (Clockwise)

Preceding gene: 23100539

Following gene: 23100541

Centisome position: 88.35

GC content: 37.72

Gene sequence:

>1140_bases
ATGAAGAGAAAAACATCTATTGCAACACTTTTTAGCATGATATTGATTGTCATGTTTAGCTATTTATTTTCACCAGCTAT
TACTCATGCTAATGGTACTGATACATACGAAGTAAGCTCTTCTACTTTAAATATTCGCAGTGCACCTTCTAATGAATCTA
GTATTGTAGGTCAATTTGTTAAAGGTAACCAACTTACGACATTCAAAGAGCAATATGACTGGGTTCAGACATATTATGGC
GGAAAAGAAGTTTGGGTAGCGAAACATCATTTAGTGCCAGTTTCAACTTCCAACGTATCTTCTACTACGTCAGAAATGAA
AGAGAACATATCTGTAACGGTTGCAGCTGAAAGTGTAATGATTCGTGCTGGAGCAGGAACGAATTATAAAAATACACACT
CTGCCTATCAGGGTGATGAATTTGATGTGATTTCATCTCAAGGTGATTGGTACCAAGTTAAATTACCAAATGGAGAAACC
GGTTGGATTGCTTCTTGGTTAACGACAGAGGTCGGTGGAGTTGTTAGCGAATCGACTGCAAGCGATAGTAGCAATTCTGA
AAGTGTTACTAACGCTCCGGAACAACAACCAGCAAGTTCGGGTTCTCTAGCTGGGTATACAATTGTTATCGATCCTGGAC
ATGGTGGTAAAGATCCTGGGTCAATTGGCCTTGGAGATGTTTGGGAGAAAGATATCGTATATAGTACTTCCAACCATGTA
GTGAATCAATTAAAAGAAGCAGGAGCCAATGTAATTACGACTAGAAGTGGAGATTATTTTGTCTCGTTAGAAGAACGTGC
ACGGATTAGTAATGATTATCAAACAGATGCATTTATAAGTATACATTATGATTCATTCCCATTACTGTCTGTTAGTGGAA
CTACAACTTATTATGCGCATAAAGAGGATCAACCTTTAGCAGCTGATATCCAGTCTTCCATTGCTTCTTCAGTAGAACTT
ACTAACCGAGGCTCTATGCAAGCAAACTATAAGGTATTAAGAGATACAACTGCGCCATCTGTACTACTCGAACTTGGATT
TATTAGTAATCAATATGACGTTAATATTGTGCAAACAGATGATTATCAACGCAGAGTAGCAACAGCAATTACCAATGGAC
TCATTCAATACTTTAAGTAA

Upstream 100 bases:

>100_bases
GATGATTTATTCGTTTTTTTATAATTATGTTTATTCTCATTATTTTTAGGTTATCCTAAGACTATCATAGAATCTAGCAA
TCTCAAAAGGGGAGGAAACG

Downstream 100 bases:

>100_bases
AAATCAATATACTATTATGTTAACTGAACTATCCTCTTCTGTGATAGTTCAGTTTTATTTTTATATTTAAAACCTTCTTT
GGCTTTACTTTTGTAGTACA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKRKTSIATLFSMILIVMFSYLFSPAITHANGTDTYEVSSSTLNIRSAPSNESSIVGQFVKGNQLTTFKEQYDWVQTYYG
GKEVWVAKHHLVPVSTSNVSSTTSEMKENISVTVAAESVMIRAGAGTNYKNTHSAYQGDEFDVISSQGDWYQVKLPNGET
GWIASWLTTEVGGVVSESTASDSSNSESVTNAPEQQPASSGSLAGYTIVIDPGHGGKDPGSIGLGDVWEKDIVYSTSNHV
VNQLKEAGANVITTRSGDYFVSLEERARISNDYQTDAFISIHYDSFPLLSVSGTTTYYAHKEDQPLAADIQSSIASSVEL
TNRGSMQANYKVLRDTTAPSVLLELGFISNQYDVNIVQTDDYQRRVATAITNGLIQYFK

Sequences:

>Translated_379_residues
MKRKTSIATLFSMILIVMFSYLFSPAITHANGTDTYEVSSSTLNIRSAPSNESSIVGQFVKGNQLTTFKEQYDWVQTYYG
GKEVWVAKHHLVPVSTSNVSSTTSEMKENISVTVAAESVMIRAGAGTNYKNTHSAYQGDEFDVISSQGDWYQVKLPNGET
GWIASWLTTEVGGVVSESTASDSSNSESVTNAPEQQPASSGSLAGYTIVIDPGHGGKDPGSIGLGDVWEKDIVYSTSNHV
VNQLKEAGANVITTRSGDYFVSLEERARISNDYQTDAFISIHYDSFPLLSVSGTTTYYAHKEDQPLAADIQSSIASSVEL
TNRGSMQANYKVLRDTTAPSVLLELGFISNQYDVNIVQTDDYQRRVATAITNGLIQYFK
>Mature_379_residues
MKRKTSIATLFSMILIVMFSYLFSPAITHANGTDTYEVSSSTLNIRSAPSNESSIVGQFVKGNQLTTFKEQYDWVQTYYG
GKEVWVAKHHLVPVSTSNVSSTTSEMKENISVTVAAESVMIRAGAGTNYKNTHSAYQGDEFDVISSQGDWYQVKLPNGET
GWIASWLTTEVGGVVSESTASDSSNSESVTNAPEQQPASSGSLAGYTIVIDPGHGGKDPGSIGLGDVWEKDIVYSTSNHV
VNQLKEAGANVITTRSGDYFVSLEERARISNDYQTDAFISIHYDSFPLLSVSGTTTYYAHKEDQPLAADIQSSIASSVEL
TNRGSMQANYKVLRDTTAPSVLLELGFISNQYDVNIVQTDDYQRRVATAITNGLIQYFK

Specific function: Probably involved in cell-wall metabolism [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=230, Percent_Identity=27.8260869565217, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790611, Length=245, Percent_Identity=27.7551020408163, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87082163, Length=230, Percent_Identity=29.1304347826087, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR017293
- InterPro:   IPR003646
- InterPro:   IPR013247 [H]

Pfam domain/function: PF01520 Amidase_3; PF08239 SH3_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 41292; Mature: 41292

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKTSIATLFSMILIVMFSYLFSPAITHANGTDTYEVSSSTLNIRSAPSNESSIVGQFV
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCEEEEECCCCCCHHHHHHEE
KGNQLTTFKEQYDWVQTYYGGKEVWVAKHHLVPVSTSNVSSTTSEMKENISVTVAAESVM
CCCCEEEHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCHHHHHHHHCCEEEEEEEEEE
IRAGAGTNYKNTHSAYQGDEFDVISSQGDWYQVKLPNGETGWIASWLTTEVGGVVSESTA
EEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCEEHHHHHHHHCCCEECCCC
SDSSNSESVTNAPEQQPASSGSLAGYTIVIDPGHGGKDPGSIGLGDVWEKDIVYSTSNHV
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECHHHH
VNQLKEAGANVITTRSGDYFVSLEERARISNDYQTDAFISIHYDSFPLLSVSGTTTYYAH
HHHHHHCCCCEEEECCCCEEEEEHHHHCCCCCCCCCEEEEEEECCCEEEEECCCEEEEEE
KEDQPLAADIQSSIASSVELTNRGSMQANYKVLRDTTAPSVLLELGFISNQYDVNIVQTD
CCCCCHHHHHHHHHHHHEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECC
DYQRRVATAITNGLIQYFK
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRKTSIATLFSMILIVMFSYLFSPAITHANGTDTYEVSSSTLNIRSAPSNESSIVGQFV
CCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEECCCEEEEECCCCCCHHHHHHEE
KGNQLTTFKEQYDWVQTYYGGKEVWVAKHHLVPVSTSNVSSTTSEMKENISVTVAAESVM
CCCCEEEHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCHHHHHHHHCCEEEEEEEEEE
IRAGAGTNYKNTHSAYQGDEFDVISSQGDWYQVKLPNGETGWIASWLTTEVGGVVSESTA
EEECCCCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCCCCEEHHHHHHHHCCCEECCCC
SDSSNSESVTNAPEQQPASSGSLAGYTIVIDPGHGGKDPGSIGLGDVWEKDIVYSTSNHV
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECHHHH
VNQLKEAGANVITTRSGDYFVSLEERARISNDYQTDAFISIHYDSFPLLSVSGTTTYYAH
HHHHHHCCCCEEEECCCCEEEEEHHHHCCCCCCCCCEEEEEEECCCEEEEECCCEEEEEE
KEDQPLAADIQSSIASSVELTNRGSMQANYKVLRDTTAPSVLLELGFISNQYDVNIVQTD
CCCCCHHHHHHHHHHHHEEECCCCCCCCCEEEEECCCCCHHHHHHHCCCCCEEEEEEECC
DYQRRVATAITNGLIQYFK
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]